Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells

The participation of type I GnRH receptor (GnRH-R) on GnRH-II-induced gonadotropin secretion in rat pituitary cells was investigated. Furthermore, we extended the study of GnRH-II action to ovarian cells. The GnRH-II was able to mobilize inositol triphosphate (IP3) and to induce LH and FSH release i...

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Autores principales:	Mongiat, L.A., Lux-Lantos, V.A., Libertun, C.
Formato:	JOUR
Materias:	Gonadotropin-releasing hormone Gonadotropin-releasing hormone receptor Neuroendocrinology Ovary Pituitary gonadorelin gonadorelin derivative gonadorelin receptor inositol trisphosphate animal cell article cell proliferation concentration response controlled study female follitropin release gonadotropin release hormonal regulation hormone action hypophysis cell luteinizing hormone release neuroendocrinology nonhuman ovary cell priority journal progesterone release rat superovulation Animals Cells, Cultured Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Hormone Antagonists Luteinizing Hormone Pituitary Gland, Anterior Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00063363_v71_n2_p464_Mongiat
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00063363_v71_n2_p464_Mongiat2023-10-03T14:04:54Z Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells Mongiat, L.A. Lux-Lantos, V.A. Libertun, C. Gonadotropin-releasing hormone Gonadotropin-releasing hormone receptor Neuroendocrinology Ovary Pituitary gonadorelin gonadorelin derivative gonadorelin receptor inositol trisphosphate animal cell article cell proliferation concentration response controlled study female follitropin release gonadotropin release hormonal regulation hormone action hypophysis cell luteinizing hormone release neuroendocrinology nonhuman ovary cell priority journal progesterone release rat superovulation Animals Cells, Cultured Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Hormone Antagonists Luteinizing Hormone Ovary Pituitary Gland, Anterior Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Animalia The participation of type I GnRH receptor (GnRH-R) on GnRH-II-induced gonadotropin secretion in rat pituitary cells was investigated. Furthermore, we extended the study of GnRH-II action to ovarian cells. The GnRH-II was able to mobilize inositol triphosphate (IP3) and to induce LH and FSH release in a dose-dependent manner in pituitary cells and in a GnRH-I-like manner. The GnRH-analog 135-18 (agonist for type II GnRH-R and antagonist for type I GnRH-R) was unable to elicit any cellular response tested in these pituitary cells. The GnRH-II responses were blocked by the type I GnRH-R-antagonists CRX or 135-18, suggesting that these effects were mediated by the type I GnRH-R. In contrast to pituitary cells, GnRH-I, but not GnRH-II, elicited an IP3 response in superovulated ovarian cells; 135-18 also had no effect. However, GnRH-II as well as GnRH-I presented antiproliferative effects on these cells. Surprisingly, 135-18 had stronger antiproliferative effects than either GnRH peptide. The 135-18 analog, but not GnRH-I or GnRH-II, increased progesterone secretion in superovulated ovarian cells. These results strongly suggest that GnRH-II is able to stimulate rat pituitary cells through the type I GnRH-R, with no evidence for the presence of type II GnRH-R. On the other hand, our results indicate a putative GnRH-R in superovulated ovarian cells with response characteristics that differ from those of the GnRH-R in the pituitary. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00063363_v71_n2_p464_Mongiat
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Gonadotropin-releasing hormone Gonadotropin-releasing hormone receptor Neuroendocrinology Ovary Pituitary gonadorelin gonadorelin derivative gonadorelin receptor inositol trisphosphate animal cell article cell proliferation concentration response controlled study female follitropin release gonadotropin release hormonal regulation hormone action hypophysis cell luteinizing hormone release neuroendocrinology nonhuman ovary cell priority journal progesterone release rat superovulation Animals Cells, Cultured Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Hormone Antagonists Luteinizing Hormone Ovary Pituitary Gland, Anterior Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Animalia
spellingShingle	Gonadotropin-releasing hormone Gonadotropin-releasing hormone receptor Neuroendocrinology Ovary Pituitary gonadorelin gonadorelin derivative gonadorelin receptor inositol trisphosphate animal cell article cell proliferation concentration response controlled study female follitropin release gonadotropin release hormonal regulation hormone action hypophysis cell luteinizing hormone release neuroendocrinology nonhuman ovary cell priority journal progesterone release rat superovulation Animals Cells, Cultured Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Hormone Antagonists Luteinizing Hormone Ovary Pituitary Gland, Anterior Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Animalia Mongiat, L.A. Lux-Lantos, V.A. Libertun, C. Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
topic_facet	Gonadotropin-releasing hormone Gonadotropin-releasing hormone receptor Neuroendocrinology Ovary Pituitary gonadorelin gonadorelin derivative gonadorelin receptor inositol trisphosphate animal cell article cell proliferation concentration response controlled study female follitropin release gonadotropin release hormonal regulation hormone action hypophysis cell luteinizing hormone release neuroendocrinology nonhuman ovary cell priority journal progesterone release rat superovulation Animals Cells, Cultured Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Hormone Antagonists Luteinizing Hormone Ovary Pituitary Gland, Anterior Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Animalia
description	The participation of type I GnRH receptor (GnRH-R) on GnRH-II-induced gonadotropin secretion in rat pituitary cells was investigated. Furthermore, we extended the study of GnRH-II action to ovarian cells. The GnRH-II was able to mobilize inositol triphosphate (IP3) and to induce LH and FSH release in a dose-dependent manner in pituitary cells and in a GnRH-I-like manner. The GnRH-analog 135-18 (agonist for type II GnRH-R and antagonist for type I GnRH-R) was unable to elicit any cellular response tested in these pituitary cells. The GnRH-II responses were blocked by the type I GnRH-R-antagonists CRX or 135-18, suggesting that these effects were mediated by the type I GnRH-R. In contrast to pituitary cells, GnRH-I, but not GnRH-II, elicited an IP3 response in superovulated ovarian cells; 135-18 also had no effect. However, GnRH-II as well as GnRH-I presented antiproliferative effects on these cells. Surprisingly, 135-18 had stronger antiproliferative effects than either GnRH peptide. The 135-18 analog, but not GnRH-I or GnRH-II, increased progesterone secretion in superovulated ovarian cells. These results strongly suggest that GnRH-II is able to stimulate rat pituitary cells through the type I GnRH-R, with no evidence for the presence of type II GnRH-R. On the other hand, our results indicate a putative GnRH-R in superovulated ovarian cells with response characteristics that differ from those of the GnRH-R in the pituitary.
format	JOUR
author	Mongiat, L.A. Lux-Lantos, V.A. Libertun, C.
author_facet	Mongiat, L.A. Lux-Lantos, V.A. Libertun, C.
author_sort	Mongiat, L.A.
title	Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
title_short	Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
title_full	Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
title_fullStr	Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
title_full_unstemmed	Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
title_sort	evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
url	http://hdl.handle.net/20.500.12110/paper_00063363_v71_n2_p464_Mongiat
work_keys_str_mv	AT mongiatla evidencefordifferentgonadotropinreleasinghormoneresponsesitesinratovarianandpituitarycells AT luxlantosva evidencefordifferentgonadotropinreleasinghormoneresponsesitesinratovarianandpituitarycells AT libertunc evidencefordifferentgonadotropinreleasinghormoneresponsesitesinratovarianandpituitarycells
_version_	1782029776383377408

Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells

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