Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats

The present work demonstrates that phenformin exerted an inducing effect on δ-amino-levulinic acid synthase (ALA-S) and ferrochelatase activities and on cytochrome P-450 content in isolated hepatocytes from rats with experimental diabetes. Similar results were obtained with respect to ALA-S activity...

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Autores principales:	Canepa, E.T., Llambias, E.B.C., Grinstein, M.
Formato:	JOUR
Materias:	5 aminolevulinate synthase allylisopropylacetamide bucladesine chlorpropamide cyclic amp cytochrome p450 ferrochelatase lead acetate phenformin phenobarbital animal cell animal experiment animal model article cell suspension drug concentration enzyme induction hepatic porphyria liver cell male nonhuman priority journal rat streptozocin diabetes 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Chlorpropamide Cyclic AMP Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase In Vitro Lead Liver Lyases Male Phenformin Rats Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00062952_v40_n2_p365_Canepa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00062952_v40_n2_p365_Canepa
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spelling	todo:paper_00062952_v40_n2_p365_Canepa2023-10-03T14:04:16Z Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats Canepa, E.T. Llambias, E.B.C. Grinstein, M. 5 aminolevulinate synthase allylisopropylacetamide bucladesine chlorpropamide cyclic amp cytochrome p450 ferrochelatase lead acetate phenformin phenobarbital animal cell animal experiment animal model article cell suspension drug concentration enzyme induction hepatic porphyria liver cell male nonhuman priority journal rat streptozocin diabetes 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Chlorpropamide Cyclic AMP Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase In Vitro Lead Liver Lyases Male Phenformin Rats Support, Non-U.S. Gov't The present work demonstrates that phenformin exerted an inducing effect on δ-amino-levulinic acid synthase (ALA-S) and ferrochelatase activities and on cytochrome P-450 content in isolated hepatocytes from rats with experimental diabetes. Similar results were obtained with respect to ALA-S activity and cytochrome P-450 content when chlorpropamide was used. The inducing effect exerted by allylisopropylacetamide (AIA) on ALA-S and ferrochelatase activities in diabetic hepatic cells was markedly greater than that observed in normal hepatocytes. This stimulatory response was not enhanced by adding dibutyryl cyclic AMP (cAMP). When phenformin was added to isolated rat hepatocytes of normal rats, induction of ALA-S and ferrochelatase activities and cytochrome P-450 content was observed only in the presence of added dibutyryl cAMP. Addition of chlorpropamide to this in vitro system did not exert an inducing effect on the same enzymes even in the presence of dibutyryl cAMP. The present results add more experimental evidence about the lability of the heme pathway of diabetic hepatocytes. © 1990. Fil:Canepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00062952_v40_n2_p365_Canepa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5 aminolevulinate synthase allylisopropylacetamide bucladesine chlorpropamide cyclic amp cytochrome p450 ferrochelatase lead acetate phenformin phenobarbital animal cell animal experiment animal model article cell suspension drug concentration enzyme induction hepatic porphyria liver cell male nonhuman priority journal rat streptozocin diabetes 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Chlorpropamide Cyclic AMP Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase In Vitro Lead Liver Lyases Male Phenformin Rats Support, Non-U.S. Gov't
spellingShingle	5 aminolevulinate synthase allylisopropylacetamide bucladesine chlorpropamide cyclic amp cytochrome p450 ferrochelatase lead acetate phenformin phenobarbital animal cell animal experiment animal model article cell suspension drug concentration enzyme induction hepatic porphyria liver cell male nonhuman priority journal rat streptozocin diabetes 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Chlorpropamide Cyclic AMP Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase In Vitro Lead Liver Lyases Male Phenformin Rats Support, Non-U.S. Gov't Canepa, E.T. Llambias, E.B.C. Grinstein, M. Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
topic_facet	5 aminolevulinate synthase allylisopropylacetamide bucladesine chlorpropamide cyclic amp cytochrome p450 ferrochelatase lead acetate phenformin phenobarbital animal cell animal experiment animal model article cell suspension drug concentration enzyme induction hepatic porphyria liver cell male nonhuman priority journal rat streptozocin diabetes 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Chlorpropamide Cyclic AMP Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase In Vitro Lead Liver Lyases Male Phenformin Rats Support, Non-U.S. Gov't
description	The present work demonstrates that phenformin exerted an inducing effect on δ-amino-levulinic acid synthase (ALA-S) and ferrochelatase activities and on cytochrome P-450 content in isolated hepatocytes from rats with experimental diabetes. Similar results were obtained with respect to ALA-S activity and cytochrome P-450 content when chlorpropamide was used. The inducing effect exerted by allylisopropylacetamide (AIA) on ALA-S and ferrochelatase activities in diabetic hepatic cells was markedly greater than that observed in normal hepatocytes. This stimulatory response was not enhanced by adding dibutyryl cyclic AMP (cAMP). When phenformin was added to isolated rat hepatocytes of normal rats, induction of ALA-S and ferrochelatase activities and cytochrome P-450 content was observed only in the presence of added dibutyryl cAMP. Addition of chlorpropamide to this in vitro system did not exert an inducing effect on the same enzymes even in the presence of dibutyryl cAMP. The present results add more experimental evidence about the lability of the heme pathway of diabetic hepatocytes. © 1990.
format	JOUR
author	Canepa, E.T. Llambias, E.B.C. Grinstein, M.
author_facet	Canepa, E.T. Llambias, E.B.C. Grinstein, M.
author_sort	Canepa, E.T.
title	Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_short	Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_full	Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_fullStr	Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_full_unstemmed	Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_sort	studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome p-450 and cyclic amp by phenformin. chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
url	http://hdl.handle.net/20.500.12110/paper_00062952_v40_n2_p365_Canepa
work_keys_str_mv	AT canepaet studiesoninductionofdaminolevulinicacidsynthaseferrochelatasecytochromep450andcyclicampbyphenforminchlorpropamideallylisopropylacetamideandleadinhepatocytesfromnormalandexperimentaldiabeticrats AT llambiasebc studiesoninductionofdaminolevulinicacidsynthaseferrochelatasecytochromep450andcyclicampbyphenforminchlorpropamideallylisopropylacetamideandleadinhepatocytesfromnormalandexperimentaldiabeticrats AT grinsteinm studiesoninductionofdaminolevulinicacidsynthaseferrochelatasecytochromep450andcyclicampbyphenforminchlorpropamideallylisopropylacetamideandleadinhepatocytesfromnormalandexperimentaldiabeticrats
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Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats

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