Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli

Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity...

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Autores principales: Algranati, I.D., Goldemberg, S.H.
Formato: JOUR
Materias:
messenger rna
polyamine
bacteriophage
escherichia coli
formylmethionyl transfer ribonucleic acid
in vitro study
microorganism
rna translation
theoretical study
Coliphages
Escherichia coli
Kinetics
Magnesium
Polyamines
Putrescine
Ribosomes
RNA, Messenger
Translation, Genetic
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0006291X_v75_n4_p1045_Algranati
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spelling todo:paper_0006291X_v75_n4_p1045_Algranati2023-10-03T14:04:09Z Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli Algranati, I.D. Goldemberg, S.H. messenger rna polyamine bacteriophage escherichia coli formylmethionyl transfer ribonucleic acid in vitro study microorganism rna translation theoretical study Coliphages Escherichia coli Kinetics Magnesium Polyamines Putrescine Ribosomes RNA, Messenger Translation, Genetic Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity for the binding of formyl-methionyl-tRNA to ribosomes, induced by the trinucleotide AUG. The analysis of polypeptides formed showed that extracts from polyamine-depleted and supplemented cells are both able to synthesize complete protein chains. © 1977. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Goldemberg, S.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic messenger rna
polyamine
bacteriophage
escherichia coli
formylmethionyl transfer ribonucleic acid
in vitro study
microorganism
rna translation
theoretical study
Coliphages
Escherichia coli
Kinetics
Magnesium
Polyamines
Putrescine
Ribosomes
RNA, Messenger
Translation, Genetic
spellingShingle messenger rna
polyamine
bacteriophage
escherichia coli
formylmethionyl transfer ribonucleic acid
in vitro study
microorganism
rna translation
theoretical study
Coliphages
Escherichia coli
Kinetics
Magnesium
Polyamines
Putrescine
Ribosomes
RNA, Messenger
Translation, Genetic
Algranati, I.D.
Goldemberg, S.H.
Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
topic_facet messenger rna
polyamine
bacteriophage
escherichia coli
formylmethionyl transfer ribonucleic acid
in vitro study
microorganism
rna translation
theoretical study
Coliphages
Escherichia coli
Kinetics
Magnesium
Polyamines
Putrescine
Ribosomes
RNA, Messenger
Translation, Genetic
description Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity for the binding of formyl-methionyl-tRNA to ribosomes, induced by the trinucleotide AUG. The analysis of polypeptides formed showed that extracts from polyamine-depleted and supplemented cells are both able to synthesize complete protein chains. © 1977.
format JOUR
author Algranati, I.D.
Goldemberg, S.H.
author_facet Algranati, I.D.
Goldemberg, S.H.
author_sort Algranati, I.D.
title Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
title_short Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
title_full Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
title_fullStr Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
title_full_unstemmed Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
title_sort translation of natural mrna in cell-free systems from a polyamine-requiring mutant of escherichia coli
url http://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati
work_keys_str_mv AT algranatiid translationofnaturalmrnaincellfreesystemsfromapolyaminerequiringmutantofescherichiacoli
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