Glycosyl transfer to an acceptor lipid from insects

Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were eq...

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Autores principales: Quesada Allué, L.A., Belocopitow, E., Maréchal, L.R.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v66_n4_p1201_QuesadaAllue
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spelling todo:paper_0006291X_v66_n4_p1201_QuesadaAllue2023-10-03T14:04:08Z Glycosyl transfer to an acceptor lipid from insects Quesada Allué, L.A. Belocopitow, E. Maréchal, L.R. lipid uridine diphosphate glucose uridine diphosphate n acetylglucosamine arthropod in vitro study liver microsome rat theoretical study Acetylglucosamine Animal Chromatography, Thin Layer Diptera Glucose Glycolipids Insects Phospholipids Rats Species Specificity Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were equal to those of dolichol monophosphate. The glycosylated compounds were acid labile. Treatment with alkali of the acetylglucosaminyl compound produced a substance that migrated like a hexose phosphate on electrophoresis and that liberated acetylglucosamine on treatment with alkaline phosphatase. The behaviour of the insect glucosylated lipid on thin-layer chromatography and its stability to phenol were similar to dolichol monophosphate glucose and different from ficaprenyl monophosphate glucose. It is concluded that the insect glycosyl acceptor lipid is an α saturated polyprenyl phosphate. © 1975. Fil:Quesada Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Belocopitow, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v66_n4_p1201_QuesadaAllue
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic lipid
uridine diphosphate glucose
uridine diphosphate n acetylglucosamine
arthropod
in vitro study
liver
microsome
rat
theoretical study
Acetylglucosamine
Animal
Chromatography, Thin Layer
Diptera
Glucose
Glycolipids
Insects
Phospholipids
Rats
Species Specificity
spellingShingle lipid
uridine diphosphate glucose
uridine diphosphate n acetylglucosamine
arthropod
in vitro study
liver
microsome
rat
theoretical study
Acetylglucosamine
Animal
Chromatography, Thin Layer
Diptera
Glucose
Glycolipids
Insects
Phospholipids
Rats
Species Specificity
Quesada Allué, L.A.
Belocopitow, E.
Maréchal, L.R.
Glycosyl transfer to an acceptor lipid from insects
topic_facet lipid
uridine diphosphate glucose
uridine diphosphate n acetylglucosamine
arthropod
in vitro study
liver
microsome
rat
theoretical study
Acetylglucosamine
Animal
Chromatography, Thin Layer
Diptera
Glucose
Glycolipids
Insects
Phospholipids
Rats
Species Specificity
description Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were equal to those of dolichol monophosphate. The glycosylated compounds were acid labile. Treatment with alkali of the acetylglucosaminyl compound produced a substance that migrated like a hexose phosphate on electrophoresis and that liberated acetylglucosamine on treatment with alkaline phosphatase. The behaviour of the insect glucosylated lipid on thin-layer chromatography and its stability to phenol were similar to dolichol monophosphate glucose and different from ficaprenyl monophosphate glucose. It is concluded that the insect glycosyl acceptor lipid is an α saturated polyprenyl phosphate. © 1975.
format JOUR
author Quesada Allué, L.A.
Belocopitow, E.
Maréchal, L.R.
author_facet Quesada Allué, L.A.
Belocopitow, E.
Maréchal, L.R.
author_sort Quesada Allué, L.A.
title Glycosyl transfer to an acceptor lipid from insects
title_short Glycosyl transfer to an acceptor lipid from insects
title_full Glycosyl transfer to an acceptor lipid from insects
title_fullStr Glycosyl transfer to an acceptor lipid from insects
title_full_unstemmed Glycosyl transfer to an acceptor lipid from insects
title_sort glycosyl transfer to an acceptor lipid from insects
url http://hdl.handle.net/20.500.12110/paper_0006291X_v66_n4_p1201_QuesadaAllue
work_keys_str_mv AT quesadaalluela glycosyltransfertoanacceptorlipidfrominsects
AT belocopitowe glycosyltransfertoanacceptorlipidfrominsects
AT marechallr glycosyltransfertoanacceptorlipidfrominsects
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