Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems

Phenylalanine incorporation into polypeptides induced by poly (U) is markedly reduced in extracts obtained from polyamine depleted bacteria, and this effect, totally independent of RNA synthesis, is related to some deficiency at the level of the ribosomal particles and/or the factors bound to them....

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Autores principales: Echandi, G., Algranati, I.D.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v62_n2_p313_Echandi
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spelling todo:paper_0006291X_v62_n2_p313_Echandi2023-10-03T14:04:08Z Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems Echandi, G. Algranati, I.D. polyamine protein putrescine spermidine auxotrophic mutant escherichia coli in vitro study microorganism phenylalanine c 14 protein synthesis ribosome theoretical study Amino Acid Activation Bacterial Proteins Cell Fractionation Centrifugation, Density Gradient Escherichia coli Kinetics Magnesium Mutation Phenylalanine Putrescine Ribosomes Time Factors Phenylalanine incorporation into polypeptides induced by poly (U) is markedly reduced in extracts obtained from polyamine depleted bacteria, and this effect, totally independent of RNA synthesis, is related to some deficiency at the level of the ribosomal particles and/or the factors bound to them. The ribosomal profiles corresponding to polyamine starved cells change when cultures are supplemented with putrescine. © 1975 Academic Press, Inc. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v62_n2_p313_Echandi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic polyamine
protein
putrescine
spermidine
auxotrophic mutant
escherichia coli
in vitro study
microorganism
phenylalanine c 14
protein synthesis
ribosome
theoretical study
Amino Acid Activation
Bacterial Proteins
Cell Fractionation
Centrifugation, Density Gradient
Escherichia coli
Kinetics
Magnesium
Mutation
Phenylalanine
Putrescine
Ribosomes
Time Factors
spellingShingle polyamine
protein
putrescine
spermidine
auxotrophic mutant
escherichia coli
in vitro study
microorganism
phenylalanine c 14
protein synthesis
ribosome
theoretical study
Amino Acid Activation
Bacterial Proteins
Cell Fractionation
Centrifugation, Density Gradient
Escherichia coli
Kinetics
Magnesium
Mutation
Phenylalanine
Putrescine
Ribosomes
Time Factors
Echandi, G.
Algranati, I.D.
Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems
topic_facet polyamine
protein
putrescine
spermidine
auxotrophic mutant
escherichia coli
in vitro study
microorganism
phenylalanine c 14
protein synthesis
ribosome
theoretical study
Amino Acid Activation
Bacterial Proteins
Cell Fractionation
Centrifugation, Density Gradient
Escherichia coli
Kinetics
Magnesium
Mutation
Phenylalanine
Putrescine
Ribosomes
Time Factors
description Phenylalanine incorporation into polypeptides induced by poly (U) is markedly reduced in extracts obtained from polyamine depleted bacteria, and this effect, totally independent of RNA synthesis, is related to some deficiency at the level of the ribosomal particles and/or the factors bound to them. The ribosomal profiles corresponding to polyamine starved cells change when cultures are supplemented with putrescine. © 1975 Academic Press, Inc.
format JOUR
author Echandi, G.
Algranati, I.D.
author_facet Echandi, G.
Algranati, I.D.
author_sort Echandi, G.
title Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems
title_short Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems
title_full Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems
title_fullStr Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems
title_full_unstemmed Protein synthesis and ribosomal distribution in a polyamine auxotroph of Escherichia coli: Studies in cell-free systems
title_sort protein synthesis and ribosomal distribution in a polyamine auxotroph of escherichia coli: studies in cell-free systems
url http://hdl.handle.net/20.500.12110/paper_0006291X_v62_n2_p313_Echandi
work_keys_str_mv AT echandig proteinsynthesisandribosomaldistributioninapolyamineauxotrophofescherichiacolistudiesincellfreesystems
AT algranatiid proteinsynthesisandribosomaldistributioninapolyamineauxotrophofescherichiacolistudiesincellfreesystems
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