Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation

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In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, an...

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Autores principales:	Ayala-Peña, V., Santillán, G., Scolaro, L.
Formato:	JOUR
Materias:	Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis 2 morpholino 8 phenylchromone adenosine triphosphate alkaline phosphatase bone morphogenetic protein 4 bone morphogenetic protein 5 bone sialo protein messenger RNA osteogenic protein 1 phosphatidylinositol 3 kinase procollagen C proteinase protein protein kinase B unclassified drug 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one adenosine 5' o (3 thiotriphosphate) adenosine 5'-O-(3-thiotriphosphate) bone morphogenetic protein chromone derivative drug derivative morpholine derivative animal cell animal experiment animal model Article bone development cell differentiation controlled study experimental infection in vitro study measles nonhuman osteoblast protein expression rat stimulation virus load animal article biosynthesis bone cell culture differentiation drug antagonism drug effect metabolism otosclerosis physiology virology virus replication Adenosine Triphosphate Alkaline Phosphatase Animals Bone and Bones Bone Morphogenetic Protein 4 Cell Differentiation Cells, Cultured Chromones Morpholines Osteogenesis Phosphatidylinositol 3-Kinases Rats Virus Replication
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v451_n4_p609_AyalaPena
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0006291X_v451_n4_p609_AyalaPena2023-10-03T14:04:02Z Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation Ayala-Peña, V. Santillán, G. Scolaro, L. Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis 2 morpholino 8 phenylchromone adenosine triphosphate alkaline phosphatase bone morphogenetic protein 4 bone morphogenetic protein 5 bone sialo protein messenger RNA osteogenic protein 1 phosphatidylinositol 3 kinase procollagen C proteinase protein protein kinase B unclassified drug 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one adenosine 5' o (3 thiotriphosphate) adenosine 5'-O-(3-thiotriphosphate) adenosine triphosphate alkaline phosphatase bone morphogenetic protein bone morphogenetic protein 4 chromone derivative drug derivative morpholine derivative phosphatidylinositol 3 kinase animal cell animal experiment animal model Article bone development cell differentiation controlled study experimental infection in vitro study measles Measles virus nonhuman osteoblast protein expression rat stimulation virus load animal article biosynthesis bone cell culture differentiation drug antagonism drug effect metabolism otosclerosis physiology virology virus replication Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis Adenosine Triphosphate Alkaline Phosphatase Animals Bone and Bones Bone Morphogenetic Protein 4 Cell Differentiation Cells, Cultured Chromones Measles virus Morpholines Osteoblasts Osteogenesis Otosclerosis Phosphatidylinositol 3-Kinases Rats Virus Replication In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, analysis of mRNAs corresponding to osteogenic differentiation markers like alkaline phosphatase (ALP), bone sialo-protein (BSP) and bone morphogenetic proteins (BMPs) 1-4-5-7 in OBCs revealed higher values (2-75-fold of increment) for infected cells in comparison with uninfected controls. Differentiation of OBCs in osteogenic medium prior to infection influenced the level of stimulation induced by MeV. Furthermore, treatment of OBCs with Ly294002, a PI3K/AKT inhibitor, increased viral titers, whereas treatment with 10 μM or 100 μM ATPγS diminished MeV multiplication. In addition, increments of osteogenic differentiation markers induced by MeV infection were not modified either by treatment with Ly294002 or ATPγS. These data provide the first evidence demonstrating that MeV can infect osteoblasts in vitro leading to osteoblastic differentiation, a key feature in bone pathogenic processes like otosclerosis. © 2014 Elsevier Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v451_n4_p609_AyalaPena
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis 2 morpholino 8 phenylchromone adenosine triphosphate alkaline phosphatase bone morphogenetic protein 4 bone morphogenetic protein 5 bone sialo protein messenger RNA osteogenic protein 1 phosphatidylinositol 3 kinase procollagen C proteinase protein protein kinase B unclassified drug 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one adenosine 5' o (3 thiotriphosphate) adenosine 5'-O-(3-thiotriphosphate) adenosine triphosphate alkaline phosphatase bone morphogenetic protein bone morphogenetic protein 4 chromone derivative drug derivative morpholine derivative phosphatidylinositol 3 kinase animal cell animal experiment animal model Article bone development cell differentiation controlled study experimental infection in vitro study measles Measles virus nonhuman osteoblast protein expression rat stimulation virus load animal article biosynthesis bone cell culture differentiation drug antagonism drug effect metabolism otosclerosis physiology virology virus replication Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis Adenosine Triphosphate Alkaline Phosphatase Animals Bone and Bones Bone Morphogenetic Protein 4 Cell Differentiation Cells, Cultured Chromones Measles virus Morpholines Osteoblasts Osteogenesis Otosclerosis Phosphatidylinositol 3-Kinases Rats Virus Replication
spellingShingle	Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis 2 morpholino 8 phenylchromone adenosine triphosphate alkaline phosphatase bone morphogenetic protein 4 bone morphogenetic protein 5 bone sialo protein messenger RNA osteogenic protein 1 phosphatidylinositol 3 kinase procollagen C proteinase protein protein kinase B unclassified drug 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one adenosine 5' o (3 thiotriphosphate) adenosine 5'-O-(3-thiotriphosphate) adenosine triphosphate alkaline phosphatase bone morphogenetic protein bone morphogenetic protein 4 chromone derivative drug derivative morpholine derivative phosphatidylinositol 3 kinase animal cell animal experiment animal model Article bone development cell differentiation controlled study experimental infection in vitro study measles Measles virus nonhuman osteoblast protein expression rat stimulation virus load animal article biosynthesis bone cell culture differentiation drug antagonism drug effect metabolism otosclerosis physiology virology virus replication Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis Adenosine Triphosphate Alkaline Phosphatase Animals Bone and Bones Bone Morphogenetic Protein 4 Cell Differentiation Cells, Cultured Chromones Measles virus Morpholines Osteoblasts Osteogenesis Otosclerosis Phosphatidylinositol 3-Kinases Rats Virus Replication Ayala-Peña, V. Santillán, G. Scolaro, L. Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
topic_facet	Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis 2 morpholino 8 phenylchromone adenosine triphosphate alkaline phosphatase bone morphogenetic protein 4 bone morphogenetic protein 5 bone sialo protein messenger RNA osteogenic protein 1 phosphatidylinositol 3 kinase procollagen C proteinase protein protein kinase B unclassified drug 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one adenosine 5' o (3 thiotriphosphate) adenosine 5'-O-(3-thiotriphosphate) adenosine triphosphate alkaline phosphatase bone morphogenetic protein bone morphogenetic protein 4 chromone derivative drug derivative morpholine derivative phosphatidylinositol 3 kinase animal cell animal experiment animal model Article bone development cell differentiation controlled study experimental infection in vitro study measles Measles virus nonhuman osteoblast protein expression rat stimulation virus load animal article biosynthesis bone cell culture differentiation drug antagonism drug effect metabolism otosclerosis physiology virology virus replication Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis Adenosine Triphosphate Alkaline Phosphatase Animals Bone and Bones Bone Morphogenetic Protein 4 Cell Differentiation Cells, Cultured Chromones Measles virus Morpholines Osteoblasts Osteogenesis Otosclerosis Phosphatidylinositol 3-Kinases Rats Virus Replication
description	In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, analysis of mRNAs corresponding to osteogenic differentiation markers like alkaline phosphatase (ALP), bone sialo-protein (BSP) and bone morphogenetic proteins (BMPs) 1-4-5-7 in OBCs revealed higher values (2-75-fold of increment) for infected cells in comparison with uninfected controls. Differentiation of OBCs in osteogenic medium prior to infection influenced the level of stimulation induced by MeV. Furthermore, treatment of OBCs with Ly294002, a PI3K/AKT inhibitor, increased viral titers, whereas treatment with 10 μM or 100 μM ATPγS diminished MeV multiplication. In addition, increments of osteogenic differentiation markers induced by MeV infection were not modified either by treatment with Ly294002 or ATPγS. These data provide the first evidence demonstrating that MeV can infect osteoblasts in vitro leading to osteoblastic differentiation, a key feature in bone pathogenic processes like otosclerosis. © 2014 Elsevier Inc.
format	JOUR
author	Ayala-Peña, V. Santillán, G. Scolaro, L.
author_facet	Ayala-Peña, V. Santillán, G. Scolaro, L.
author_sort	Ayala-Peña, V.
title	Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_short	Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_full	Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_fullStr	Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_full_unstemmed	Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_sort	experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
url	http://hdl.handle.net/20.500.12110/paper_0006291X_v451_n4_p609_AyalaPena
work_keys_str_mv	AT ayalapenav experimentalinvitroinfectionofratosteoblastswithmeaslesvirusstimulatesosteogenicdifferentiation AT santillang experimentalinvitroinfectionofratosteoblastswithmeaslesvirusstimulatesosteogenicdifferentiation AT scolarol experimentalinvitroinfectionofratosteoblastswithmeaslesvirusstimulatesosteogenicdifferentiation
_version_	1807316055909990400

Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation

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