Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss
The Asr gene family is present in Spermatophyta. Its members are generally activated under water stress. We present evidence that tomato ASR1, one of the proteins of the family, accumulates in seed during late stages of embryogenesis, a physiological process characterized by water loss. In vitro, el...
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todo:paper_0006291X_v352_n4_p831_Maskin2023-10-03T14:03:52Z Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss Maskin, L. Frankel, N. Gudesblat, G. Demergasso, M.J. Pietrasanta, L.I. Iusem, N.D. Asr genes Atomic force microscopy Dimerization Lycopersicon esculentum Seed dimer double stranded DNA homodimer monomer protein asr1 vegetable protein article atomic force microscopy chemical bond dimerization DNA binding electrophoresis embryo development gene activation hydrophilicity in vitro study multigene family nonhuman plant seed plant tissue priority journal protein assembly protein DNA interaction protein structure water loss Dimerization DNA-Binding Proteins Lycopersicon esculentum Microscopy, Atomic Force Plant Proteins Plasmids Protein Binding Seeds Water Lycopersicon esculentum Spermatophyta The Asr gene family is present in Spermatophyta. Its members are generally activated under water stress. We present evidence that tomato ASR1, one of the proteins of the family, accumulates in seed during late stages of embryogenesis, a physiological process characterized by water loss. In vitro, electrophoretic assays show a homo-dimeric structure for ASR1 and highlight strong non-covalent interactions between monomers prone to self-assemble. Direct visualization of single molecules by atomic force microscopy (AFM) confirms that ASR1 forms homodimers and that uncovers both monomers and dimers bind double stranded DNA. © 2006 Elsevier Inc. All rights reserved. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Frankel, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gudesblat, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Demergasso, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v352_n4_p831_Maskin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Asr genes Atomic force microscopy Dimerization Lycopersicon esculentum Seed dimer double stranded DNA homodimer monomer protein asr1 vegetable protein article atomic force microscopy chemical bond dimerization DNA binding electrophoresis embryo development gene activation hydrophilicity in vitro study multigene family nonhuman plant seed plant tissue priority journal protein assembly protein DNA interaction protein structure water loss Dimerization DNA-Binding Proteins Lycopersicon esculentum Microscopy, Atomic Force Plant Proteins Plasmids Protein Binding Seeds Water Lycopersicon esculentum Spermatophyta |
| spellingShingle |
Asr genes Atomic force microscopy Dimerization Lycopersicon esculentum Seed dimer double stranded DNA homodimer monomer protein asr1 vegetable protein article atomic force microscopy chemical bond dimerization DNA binding electrophoresis embryo development gene activation hydrophilicity in vitro study multigene family nonhuman plant seed plant tissue priority journal protein assembly protein DNA interaction protein structure water loss Dimerization DNA-Binding Proteins Lycopersicon esculentum Microscopy, Atomic Force Plant Proteins Plasmids Protein Binding Seeds Water Lycopersicon esculentum Spermatophyta Maskin, L. Frankel, N. Gudesblat, G. Demergasso, M.J. Pietrasanta, L.I. Iusem, N.D. Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss |
| topic_facet |
Asr genes Atomic force microscopy Dimerization Lycopersicon esculentum Seed dimer double stranded DNA homodimer monomer protein asr1 vegetable protein article atomic force microscopy chemical bond dimerization DNA binding electrophoresis embryo development gene activation hydrophilicity in vitro study multigene family nonhuman plant seed plant tissue priority journal protein assembly protein DNA interaction protein structure water loss Dimerization DNA-Binding Proteins Lycopersicon esculentum Microscopy, Atomic Force Plant Proteins Plasmids Protein Binding Seeds Water Lycopersicon esculentum Spermatophyta |
| description |
The Asr gene family is present in Spermatophyta. Its members are generally activated under water stress. We present evidence that tomato ASR1, one of the proteins of the family, accumulates in seed during late stages of embryogenesis, a physiological process characterized by water loss. In vitro, electrophoretic assays show a homo-dimeric structure for ASR1 and highlight strong non-covalent interactions between monomers prone to self-assemble. Direct visualization of single molecules by atomic force microscopy (AFM) confirms that ASR1 forms homodimers and that uncovers both monomers and dimers bind double stranded DNA. © 2006 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Maskin, L. Frankel, N. Gudesblat, G. Demergasso, M.J. Pietrasanta, L.I. Iusem, N.D. |
| author_facet |
Maskin, L. Frankel, N. Gudesblat, G. Demergasso, M.J. Pietrasanta, L.I. Iusem, N.D. |
| author_sort |
Maskin, L. |
| title |
Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss |
| title_short |
Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss |
| title_full |
Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss |
| title_fullStr |
Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss |
| title_full_unstemmed |
Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss |
| title_sort |
dimerization and dna-binding of asr1, a small hydrophilic protein abundant in plant tissues suffering from water loss |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v352_n4_p831_Maskin |
| work_keys_str_mv |
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