1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties
We have reported the isolation of the tetranortriterpenoid 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM) from partially purified leaf extracts of Melia azedarach L. (MA) that reduced both, vesicular stomatitis virus (VSV) and Herpes simplex virus type 1 (HSV-1) multiplication. CDM blocks VSV entry and...
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todo:paper_0006291X_v344_n3_p955_Barquero2023-10-03T14:03:52Z 1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties Barquero, A.A. Michelini, F.M. Alché, L.E. 1-Cinnamoyl-3,11-dihydroxymeliacarpin Antiinflammatory Herpes simplex virus type 1 Melia azedarach L. Natural antiviral NF-κB Triterpenoids 1 cinnamoyl 3,11 dihydroxymeliacarpin antivirus agent terpenoid derivative unclassified drug 1 cinnamoyl 3,11 dihydroxymeliacarpin 1-cinnamoyl-3,11-dihydroxymeliacarpin antivirus agent immunoglobulin enhancer binding protein limonoid animal cell article controlled study DNA replication DNA virus Golgi complex Herpes simplex virus host cell immunomodulation membrane transport nonhuman priority journal purification RNA replication RNA virus Vesicular stomatitis virus virogenesis animal cell line Cercopithecus conjunctiva dose response drug effect growth, development and aging Herpes simplex virus 1 human metabolism physiology Vero cell virology virus replication Human herpesvirus 1 Melia azedarach RNA viruses Vesicular stomatitis virus Animals Antiviral Agents Cell Line Cercopithecus aethiops Conjunctiva Dose-Response Relationship, Drug Herpesvirus 1, Human Humans Limonins NF-kappa B Vero Cells Virus Replication We have reported the isolation of the tetranortriterpenoid 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM) from partially purified leaf extracts of Melia azedarach L. (MA) that reduced both, vesicular stomatitis virus (VSV) and Herpes simplex virus type 1 (HSV-1) multiplication. CDM blocks VSV entry and the intracellular transport of VSV-G protein, confining it to the Golgi apparatus, by pre- or post-treatment, respectively. Here, we report that HSV-1 glycoproteins were also confined to the Golgi apparatus independently of the nature of the host cell. Considering that MA could be acting as an immunomodulator preventing the development of herpetic stromal keratitis in mice, we also examined an eventual effect of CDM on NF-κB signaling pathway. CDM is able to impede NF-κB activation in HSV-1-infected conjunctival cells and leads to the accumulation of p65 NF-κB subunit in the cytoplasm of uninfected treated Vero cells. In conclusion, CDM is a pleiotropic agent that not only inhibits the multiplication of DNA and RNA viruses by the same mechanism of action but also modulates the NF-κB signaling pathway. © 2006 Elsevier Inc. All rights reserved. Fil:Barquero, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Michelini, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alché, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v344_n3_p955_Barquero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
1-Cinnamoyl-3,11-dihydroxymeliacarpin Antiinflammatory Herpes simplex virus type 1 Melia azedarach L. Natural antiviral NF-κB Triterpenoids 1 cinnamoyl 3,11 dihydroxymeliacarpin antivirus agent terpenoid derivative unclassified drug 1 cinnamoyl 3,11 dihydroxymeliacarpin 1-cinnamoyl-3,11-dihydroxymeliacarpin antivirus agent immunoglobulin enhancer binding protein limonoid animal cell article controlled study DNA replication DNA virus Golgi complex Herpes simplex virus host cell immunomodulation membrane transport nonhuman priority journal purification RNA replication RNA virus Vesicular stomatitis virus virogenesis animal cell line Cercopithecus conjunctiva dose response drug effect growth, development and aging Herpes simplex virus 1 human metabolism physiology Vero cell virology virus replication Human herpesvirus 1 Melia azedarach RNA viruses Vesicular stomatitis virus Animals Antiviral Agents Cell Line Cercopithecus aethiops Conjunctiva Dose-Response Relationship, Drug Herpesvirus 1, Human Humans Limonins NF-kappa B Vero Cells Virus Replication |
| spellingShingle |
1-Cinnamoyl-3,11-dihydroxymeliacarpin Antiinflammatory Herpes simplex virus type 1 Melia azedarach L. Natural antiviral NF-κB Triterpenoids 1 cinnamoyl 3,11 dihydroxymeliacarpin antivirus agent terpenoid derivative unclassified drug 1 cinnamoyl 3,11 dihydroxymeliacarpin 1-cinnamoyl-3,11-dihydroxymeliacarpin antivirus agent immunoglobulin enhancer binding protein limonoid animal cell article controlled study DNA replication DNA virus Golgi complex Herpes simplex virus host cell immunomodulation membrane transport nonhuman priority journal purification RNA replication RNA virus Vesicular stomatitis virus virogenesis animal cell line Cercopithecus conjunctiva dose response drug effect growth, development and aging Herpes simplex virus 1 human metabolism physiology Vero cell virology virus replication Human herpesvirus 1 Melia azedarach RNA viruses Vesicular stomatitis virus Animals Antiviral Agents Cell Line Cercopithecus aethiops Conjunctiva Dose-Response Relationship, Drug Herpesvirus 1, Human Humans Limonins NF-kappa B Vero Cells Virus Replication Barquero, A.A. Michelini, F.M. Alché, L.E. 1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties |
| topic_facet |
1-Cinnamoyl-3,11-dihydroxymeliacarpin Antiinflammatory Herpes simplex virus type 1 Melia azedarach L. Natural antiviral NF-κB Triterpenoids 1 cinnamoyl 3,11 dihydroxymeliacarpin antivirus agent terpenoid derivative unclassified drug 1 cinnamoyl 3,11 dihydroxymeliacarpin 1-cinnamoyl-3,11-dihydroxymeliacarpin antivirus agent immunoglobulin enhancer binding protein limonoid animal cell article controlled study DNA replication DNA virus Golgi complex Herpes simplex virus host cell immunomodulation membrane transport nonhuman priority journal purification RNA replication RNA virus Vesicular stomatitis virus virogenesis animal cell line Cercopithecus conjunctiva dose response drug effect growth, development and aging Herpes simplex virus 1 human metabolism physiology Vero cell virology virus replication Human herpesvirus 1 Melia azedarach RNA viruses Vesicular stomatitis virus Animals Antiviral Agents Cell Line Cercopithecus aethiops Conjunctiva Dose-Response Relationship, Drug Herpesvirus 1, Human Humans Limonins NF-kappa B Vero Cells Virus Replication |
| description |
We have reported the isolation of the tetranortriterpenoid 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM) from partially purified leaf extracts of Melia azedarach L. (MA) that reduced both, vesicular stomatitis virus (VSV) and Herpes simplex virus type 1 (HSV-1) multiplication. CDM blocks VSV entry and the intracellular transport of VSV-G protein, confining it to the Golgi apparatus, by pre- or post-treatment, respectively. Here, we report that HSV-1 glycoproteins were also confined to the Golgi apparatus independently of the nature of the host cell. Considering that MA could be acting as an immunomodulator preventing the development of herpetic stromal keratitis in mice, we also examined an eventual effect of CDM on NF-κB signaling pathway. CDM is able to impede NF-κB activation in HSV-1-infected conjunctival cells and leads to the accumulation of p65 NF-κB subunit in the cytoplasm of uninfected treated Vero cells. In conclusion, CDM is a pleiotropic agent that not only inhibits the multiplication of DNA and RNA viruses by the same mechanism of action but also modulates the NF-κB signaling pathway. © 2006 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Barquero, A.A. Michelini, F.M. Alché, L.E. |
| author_facet |
Barquero, A.A. Michelini, F.M. Alché, L.E. |
| author_sort |
Barquero, A.A. |
| title |
1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties |
| title_short |
1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties |
| title_full |
1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties |
| title_fullStr |
1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties |
| title_full_unstemmed |
1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κB modulating properties |
| title_sort |
1-cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-κb modulating properties |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v344_n3_p955_Barquero |
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