Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity

Heparin and Concanavalin A complexes were studied under different conditions. Interaction was measured by reading the turbidity at 420 nm. The influence of the pH, heparin, and salt concentration was measured. In the presence of salts pH was very critical, and above pH 5.4 the interaction was practi...

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Autores principales: Fernandez de Recondo, M.E., Legorburu, C., Monge, J.C., Recondo, E.F.
Formato: JOUR
Materias:
concanavalin a
heparin
anticoagulation
chemical interaction
priority journal
Blood Coagulation Tests
Calibration
Concanavalin A
Drug Interactions
Fractional Precipitation
Heparin
Hydrogen-Ion Concentration
Salts
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v155_n1_p216_FernandezdeRecondo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0006291X_v155_n1_p216_FernandezdeRecondo
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spelling todo:paper_0006291X_v155_n1_p216_FernandezdeRecondo2023-10-03T14:03:41Z Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity Fernandez de Recondo, M.E. Legorburu, C. Monge, J.C. Recondo, E.F. concanavalin a heparin anticoagulation chemical interaction priority journal Blood Coagulation Tests Calibration Concanavalin A Drug Interactions Fractional Precipitation Heparin Hydrogen-Ion Concentration Salts Support, Non-U.S. Gov't Heparin and Concanavalin A complexes were studied under different conditions. Interaction was measured by reading the turbidity at 420 nm. The influence of the pH, heparin, and salt concentration was measured. In the presence of salts pH was very critical, and above pH 5.4 the interaction was practically negligible. At pH 4.6 or 5.2 and the lowest salt concentration compatible with buffering, heparin fractions with different anticoagulant specific activities were detected in the precipitate and in the supernatant, after the interaction. In all cases a significative difference was observed in favor of the heparin isolated from the precipitate. Possibility of an artifact was eliminated by using adequates blanks and running the coagulation tests in the presence of an excess of Concanavalin A. © 1988 Academic Press, Inc. Fil:Recondo, E.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v155_n1_p216_FernandezdeRecondo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic concanavalin a
heparin
anticoagulation
chemical interaction
priority journal
Blood Coagulation Tests
Calibration
Concanavalin A
Drug Interactions
Fractional Precipitation
Heparin
Hydrogen-Ion Concentration
Salts
Support, Non-U.S. Gov't
spellingShingle concanavalin a
heparin
anticoagulation
chemical interaction
priority journal
Blood Coagulation Tests
Calibration
Concanavalin A
Drug Interactions
Fractional Precipitation
Heparin
Hydrogen-Ion Concentration
Salts
Support, Non-U.S. Gov't
Fernandez de Recondo, M.E.
Legorburu, C.
Monge, J.C.
Recondo, E.F.
Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity
topic_facet concanavalin a
heparin
anticoagulation
chemical interaction
priority journal
Blood Coagulation Tests
Calibration
Concanavalin A
Drug Interactions
Fractional Precipitation
Heparin
Hydrogen-Ion Concentration
Salts
Support, Non-U.S. Gov't
description Heparin and Concanavalin A complexes were studied under different conditions. Interaction was measured by reading the turbidity at 420 nm. The influence of the pH, heparin, and salt concentration was measured. In the presence of salts pH was very critical, and above pH 5.4 the interaction was practically negligible. At pH 4.6 or 5.2 and the lowest salt concentration compatible with buffering, heparin fractions with different anticoagulant specific activities were detected in the precipitate and in the supernatant, after the interaction. In all cases a significative difference was observed in favor of the heparin isolated from the precipitate. Possibility of an artifact was eliminated by using adequates blanks and running the coagulation tests in the presence of an excess of Concanavalin A. © 1988 Academic Press, Inc.
format JOUR
author Fernandez de Recondo, M.E.
Legorburu, C.
Monge, J.C.
Recondo, E.F.
author_facet Fernandez de Recondo, M.E.
Legorburu, C.
Monge, J.C.
Recondo, E.F.
author_sort Fernandez de Recondo, M.E.
title Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity
title_short Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity
title_full Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity
title_fullStr Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity
title_full_unstemmed Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity
title_sort heparin and concanavalin a interaction: isolation of fraction with higher anticoagulant activity
url http://hdl.handle.net/20.500.12110/paper_0006291X_v155_n1_p216_FernandezdeRecondo
work_keys_str_mv AT fernandezderecondome heparinandconcanavalinainteractionisolationoffractionwithhigheranticoagulantactivity
AT legorburuc heparinandconcanavalinainteractionisolationoffractionwithhigheranticoagulantactivity
AT mongejc heparinandconcanavalinainteractionisolationoffractionwithhigheranticoagulantactivity
AT recondoef heparinandconcanavalinainteractionisolationoffractionwithhigheranticoagulantactivity
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