Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria
The effect of streptomycin and other aminoglycosides on protein synthesis has been studied using various streptomycin-sensitive strains unable to synthesize polyamines. We have confirmed and extended our previous results showing that the strong inhibition of translation caused by the antibiotic in p...
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todo:paper_0006291X_v150_n3_p947_Nastri2023-10-03T14:03:41Z Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria Nastri, H.G. Algranati, I.D. kanamycin neomycin putrescine streptomycin escherichia coli protein synthesis Antibiotics, Aminoglycoside Bacterial Proteins Electrophoresis, Polyacrylamide Gel Escherichia coli Kinetics Molecular Weight Polyamines Putrescine Streptomycin Succinates Succinic Acid Support, Non-U.S. Gov't Translation, Genetic The effect of streptomycin and other aminoglycosides on protein synthesis has been studied using various streptomycin-sensitive strains unable to synthesize polyamines. We have confirmed and extended our previous results showing that the strong inhibition of translation caused by the antibiotic in polyamine-supplemented bacteria was markedly reduced in polyamine-starved cells. The analysis of polypeptides synthesized in the absence and presence of streptomycin in bacteria grown with and without putrescine has shown that the antibiotic provoked the accumulation of low molecular weight peptides partially bound to ribosomes in polyamine-unstarved cells. On the contrary, the drug did not induce major alterations in the patterns of proteins obtained from polyamine-depleted bacteria. The addition of the antibiotic did not evoke any change of proteolytic activity. © 1988. Fil:Nastri, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v150_n3_p947_Nastri |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
kanamycin neomycin putrescine streptomycin escherichia coli protein synthesis Antibiotics, Aminoglycoside Bacterial Proteins Electrophoresis, Polyacrylamide Gel Escherichia coli Kinetics Molecular Weight Polyamines Putrescine Streptomycin Succinates Succinic Acid Support, Non-U.S. Gov't Translation, Genetic |
| spellingShingle |
kanamycin neomycin putrescine streptomycin escherichia coli protein synthesis Antibiotics, Aminoglycoside Bacterial Proteins Electrophoresis, Polyacrylamide Gel Escherichia coli Kinetics Molecular Weight Polyamines Putrescine Streptomycin Succinates Succinic Acid Support, Non-U.S. Gov't Translation, Genetic Nastri, H.G. Algranati, I.D. Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
| topic_facet |
kanamycin neomycin putrescine streptomycin escherichia coli protein synthesis Antibiotics, Aminoglycoside Bacterial Proteins Electrophoresis, Polyacrylamide Gel Escherichia coli Kinetics Molecular Weight Polyamines Putrescine Streptomycin Succinates Succinic Acid Support, Non-U.S. Gov't Translation, Genetic |
| description |
The effect of streptomycin and other aminoglycosides on protein synthesis has been studied using various streptomycin-sensitive strains unable to synthesize polyamines. We have confirmed and extended our previous results showing that the strong inhibition of translation caused by the antibiotic in polyamine-supplemented bacteria was markedly reduced in polyamine-starved cells. The analysis of polypeptides synthesized in the absence and presence of streptomycin in bacteria grown with and without putrescine has shown that the antibiotic provoked the accumulation of low molecular weight peptides partially bound to ribosomes in polyamine-unstarved cells. On the contrary, the drug did not induce major alterations in the patterns of proteins obtained from polyamine-depleted bacteria. The addition of the antibiotic did not evoke any change of proteolytic activity. © 1988. |
| format |
JOUR |
| author |
Nastri, H.G. Algranati, I.D. |
| author_facet |
Nastri, H.G. Algranati, I.D. |
| author_sort |
Nastri, H.G. |
| title |
Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
| title_short |
Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
| title_full |
Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
| title_fullStr |
Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
| title_full_unstemmed |
Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
| title_sort |
inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v150_n3_p947_Nastri |
| work_keys_str_mv |
AT nastrihg inhibitionofproteinsynthesisbyaminoglycosideantibioticsinpolyaminerequiringbacteria AT algranatiid inhibitionofproteinsynthesisbyaminoglycosideantibioticsinpolyaminerequiringbacteria |
| _version_ |
1807318660517199872 |