An intermediate in cyclic β1-2 glucan biosynthesis

Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic β1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic β1-2...

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Autores principales:	Zorreguieta, A., Ugalde, R.A., Leloir, L.F.
Formato:	JOUR
Materias:	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p352_Zorreguieta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0006291X_v126_n1_p352_Zorreguieta
record_format	dspace
spelling	todo:paper_0006291X_v126_n1_p352_Zorreguieta2023-10-03T14:03:37Z An intermediate in cyclic β1-2 glucan biosynthesis Zorreguieta, A. Ugalde, R.A. Leloir, L.F. radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic β1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic β1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive β1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of β-elimination leads to the partial release of free saccharides (about 30%). It is concluded that β1-2 glucan chains are built up joined to a protein and then released as free cyclic β1-2 glucan. © 1985. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p352_Zorreguieta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical
spellingShingle	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical Zorreguieta, A. Ugalde, R.A. Leloir, L.F. An intermediate in cyclic β1-2 glucan biosynthesis
topic_facet	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical
description	Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic β1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic β1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive β1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of β-elimination leads to the partial release of free saccharides (about 30%). It is concluded that β1-2 glucan chains are built up joined to a protein and then released as free cyclic β1-2 glucan. © 1985.
format	JOUR
author	Zorreguieta, A. Ugalde, R.A. Leloir, L.F.
author_facet	Zorreguieta, A. Ugalde, R.A. Leloir, L.F.
author_sort	Zorreguieta, A.
title	An intermediate in cyclic β1-2 glucan biosynthesis
title_short	An intermediate in cyclic β1-2 glucan biosynthesis
title_full	An intermediate in cyclic β1-2 glucan biosynthesis
title_fullStr	An intermediate in cyclic β1-2 glucan biosynthesis
title_full_unstemmed	An intermediate in cyclic β1-2 glucan biosynthesis
title_sort	intermediate in cyclic β1-2 glucan biosynthesis
url	http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p352_Zorreguieta
work_keys_str_mv	AT zorreguietaa anintermediateincyclicb12glucanbiosynthesis AT ugaldera anintermediateincyclicb12glucanbiosynthesis AT leloirlf anintermediateincyclicb12glucanbiosynthesis AT zorreguietaa intermediateincyclicb12glucanbiosynthesis AT ugaldera intermediateincyclicb12glucanbiosynthesis AT leloirlf intermediateincyclicb12glucanbiosynthesis
_version_	1807319149828898816

An intermediate in cyclic β1-2 glucan biosynthesis

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