Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria

Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemente...

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Autores principales: Algranati, I.D., Goldemberg, S.H.
Formato: JOUR
Materias:
putrescine
spermidine
spermine
animal experiment
auxotrophic mutant
escherichia coli
heredity
in vitro study
protein synthesis
Cell-Free System
Escherichia coli
Kinetics
Mutation
Peptide Chain Elongation
Peptide Chain Initiation
Peptide Chain Termination
Putrescine
Ribosomes
RNA, Transfer, Amino Acyl
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v103_n1_p8_Algranati
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0006291X_v103_n1_p8_Algranati
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spelling todo:paper_0006291X_v103_n1_p8_Algranati2023-10-03T14:03:35Z Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria Algranati, I.D. Goldemberg, S.H. putrescine spermidine spermine animal experiment auxotrophic mutant escherichia coli heredity in vitro study protein synthesis Cell-Free System Escherichia coli Kinetics Mutation Peptide Chain Elongation Peptide Chain Initiation Peptide Chain Termination Putrescine Ribosomes RNA, Transfer, Amino Acyl Support, Non-U.S. Gov't Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemented bacteria. The markedly reduced activity in systems from polyamine starved cells is most probably due to a poorly efficient initiation step. The estimation of nascent peptide chains and their release from tRNA support the idea that polypeptide elongation and termination processes do not have decreased rates in systems from polyamine-depleted bacteria. © 1981. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Goldemberg, S.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v103_n1_p8_Algranati
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic putrescine
spermidine
spermine
animal experiment
auxotrophic mutant
escherichia coli
heredity
in vitro study
protein synthesis
Cell-Free System
Escherichia coli
Kinetics
Mutation
Peptide Chain Elongation
Peptide Chain Initiation
Peptide Chain Termination
Putrescine
Ribosomes
RNA, Transfer, Amino Acyl
Support, Non-U.S. Gov't
spellingShingle putrescine
spermidine
spermine
animal experiment
auxotrophic mutant
escherichia coli
heredity
in vitro study
protein synthesis
Cell-Free System
Escherichia coli
Kinetics
Mutation
Peptide Chain Elongation
Peptide Chain Initiation
Peptide Chain Termination
Putrescine
Ribosomes
RNA, Transfer, Amino Acyl
Support, Non-U.S. Gov't
Algranati, I.D.
Goldemberg, S.H.
Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
topic_facet putrescine
spermidine
spermine
animal experiment
auxotrophic mutant
escherichia coli
heredity
in vitro study
protein synthesis
Cell-Free System
Escherichia coli
Kinetics
Mutation
Peptide Chain Elongation
Peptide Chain Initiation
Peptide Chain Termination
Putrescine
Ribosomes
RNA, Transfer, Amino Acyl
Support, Non-U.S. Gov't
description Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemented bacteria. The markedly reduced activity in systems from polyamine starved cells is most probably due to a poorly efficient initiation step. The estimation of nascent peptide chains and their release from tRNA support the idea that polypeptide elongation and termination processes do not have decreased rates in systems from polyamine-depleted bacteria. © 1981.
format JOUR
author Algranati, I.D.
Goldemberg, S.H.
author_facet Algranati, I.D.
Goldemberg, S.H.
author_sort Algranati, I.D.
title Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
title_short Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
title_full Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
title_fullStr Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
title_full_unstemmed Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
title_sort initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
url http://hdl.handle.net/20.500.12110/paper_0006291X_v103_n1_p8_Algranati
work_keys_str_mv AT algranatiid initiationelongationandterminationofpolypeptidesynthesisincellfreesystemsfrompolyaminedeficientbacteria
AT goldembergsh initiationelongationandterminationofpolypeptidesynthesisincellfreesystemsfrompolyaminedeficientbacteria
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