Structural and dynamical surface properties of phosphatidylethanolamine containing membranes

The hydration of solid dimyristoylphosphatidylethanolamine (DMPE) produces a negligible shift in the asymmetric stretching frequency of the phosphate groups in contrast to dimyristoylphosphatidylcholine (DMPC). This suggests that the hydration of DMPE is not a consequence of the disruption of the so...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bouchet, A.M., Frías, M.A., Lairion, F., Martini, F., Almaleck, H., Gordillo, G., Disalvo, E.A.
Formato:	JOUR
Materias:	Cyclic voltammetry Dipole potential Interphase water activity Monolayer Phosphate asymmetric frequency Phosphatidylethanolamine Protein penetration ammonia dimyristoylphosphatidylcholine dimyristoylphosphatidylethanolamine ethanolamine derivative lipid long chain fatty acid coenzyme A ligase nitrogen phosphate phosphatidylcholine phosphatidylethanolamine phosphorus protein unclassified drug article cell disruption cell energy controlled study correlation analysis dipole electric field energy absorption gene translocation hydration interface pressure interphase membrane biology membrane structure molecular dynamics molecular interaction monolayer culture polarization priority journal surface property water content Adsorption Animals Biophysical Phenomena Lecithins Membrane Lipids Membrane Potentials Membranes, Artificial Micelles Molecular Structure Phosphatidylethanolamines Pressure Proteins Spectroscopy, Fourier Transform Infrared Surface Properties Thermodynamics
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00052736_v1788_n5_p918_Bouchet
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00052736_v1788_n5_p918_Bouchet
record_format	dspace
spelling	todo:paper_00052736_v1788_n5_p918_Bouchet2023-10-03T14:03:16Z Structural and dynamical surface properties of phosphatidylethanolamine containing membranes Bouchet, A.M. Frías, M.A. Lairion, F. Martini, F. Almaleck, H. Gordillo, G. Disalvo, E.A. Cyclic voltammetry Dipole potential Interphase water activity Monolayer Phosphate asymmetric frequency Phosphatidylethanolamine Protein penetration ammonia dimyristoylphosphatidylcholine dimyristoylphosphatidylethanolamine ethanolamine derivative lipid long chain fatty acid coenzyme A ligase nitrogen phosphate phosphatidylcholine phosphatidylethanolamine phosphorus protein unclassified drug article cell disruption cell energy controlled study correlation analysis dipole electric field energy absorption gene translocation hydration interface pressure interphase membrane biology membrane structure molecular dynamics molecular interaction monolayer culture polarization priority journal surface property water content Adsorption Animals Biophysical Phenomena Lecithins Membrane Lipids Membrane Potentials Membranes, Artificial Micelles Molecular Structure Phosphatidylethanolamines Pressure Proteins Spectroscopy, Fourier Transform Infrared Surface Properties Thermodynamics The hydration of solid dimyristoylphosphatidylethanolamine (DMPE) produces a negligible shift in the asymmetric stretching frequency of the phosphate groups in contrast to dimyristoylphosphatidylcholine (DMPC). This suggests that the hydration of DMPE is not a consequence of the disruption of the solid lattice of the phosphate groups as occurs in DMPC. The strong lateral interactions between NH3 and PO2 - groups present in the solid PEs remain when the lipids are fully hydrated and seem to be a limiting factor for the hydration of the phosphate group hindering the reorientation of the polar heads. The lower mobility is reflected in a higher energy to translocate the phosphoethanolamine (P-N) dipoles in an electrical field. This energy is decreased in the presence of increasing ratios of PCs of saturated chains in phosphoethanolamine monolayer. The association of PC and PE in the membrane affecting the reorientation of the P-N groups is dependent of the chain-chain interaction. The dipole potentials of PCs and PEs mixtures show different behaviors according to the saturation of the acyl chain. This was correlated with the area in monolayers and the hydration of the P-N groups. In spite of the low hydration, DMPE is still able to adsorb fully hydrated proteins, although in a lower rate than DMPC at the same surface pressure. This indicates that PE interfaces posses an excess of surface free energy to drive protein interaction. The relation of this free energy with the low water content is discussed. © 2009 Elsevier B.V. All rights reserved. Fil:Gordillo, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052736_v1788_n5_p918_Bouchet
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cyclic voltammetry Dipole potential Interphase water activity Monolayer Phosphate asymmetric frequency Phosphatidylethanolamine Protein penetration ammonia dimyristoylphosphatidylcholine dimyristoylphosphatidylethanolamine ethanolamine derivative lipid long chain fatty acid coenzyme A ligase nitrogen phosphate phosphatidylcholine phosphatidylethanolamine phosphorus protein unclassified drug article cell disruption cell energy controlled study correlation analysis dipole electric field energy absorption gene translocation hydration interface pressure interphase membrane biology membrane structure molecular dynamics molecular interaction monolayer culture polarization priority journal surface property water content Adsorption Animals Biophysical Phenomena Lecithins Membrane Lipids Membrane Potentials Membranes, Artificial Micelles Molecular Structure Phosphatidylethanolamines Pressure Proteins Spectroscopy, Fourier Transform Infrared Surface Properties Thermodynamics
spellingShingle	Cyclic voltammetry Dipole potential Interphase water activity Monolayer Phosphate asymmetric frequency Phosphatidylethanolamine Protein penetration ammonia dimyristoylphosphatidylcholine dimyristoylphosphatidylethanolamine ethanolamine derivative lipid long chain fatty acid coenzyme A ligase nitrogen phosphate phosphatidylcholine phosphatidylethanolamine phosphorus protein unclassified drug article cell disruption cell energy controlled study correlation analysis dipole electric field energy absorption gene translocation hydration interface pressure interphase membrane biology membrane structure molecular dynamics molecular interaction monolayer culture polarization priority journal surface property water content Adsorption Animals Biophysical Phenomena Lecithins Membrane Lipids Membrane Potentials Membranes, Artificial Micelles Molecular Structure Phosphatidylethanolamines Pressure Proteins Spectroscopy, Fourier Transform Infrared Surface Properties Thermodynamics Bouchet, A.M. Frías, M.A. Lairion, F. Martini, F. Almaleck, H. Gordillo, G. Disalvo, E.A. Structural and dynamical surface properties of phosphatidylethanolamine containing membranes
topic_facet	Cyclic voltammetry Dipole potential Interphase water activity Monolayer Phosphate asymmetric frequency Phosphatidylethanolamine Protein penetration ammonia dimyristoylphosphatidylcholine dimyristoylphosphatidylethanolamine ethanolamine derivative lipid long chain fatty acid coenzyme A ligase nitrogen phosphate phosphatidylcholine phosphatidylethanolamine phosphorus protein unclassified drug article cell disruption cell energy controlled study correlation analysis dipole electric field energy absorption gene translocation hydration interface pressure interphase membrane biology membrane structure molecular dynamics molecular interaction monolayer culture polarization priority journal surface property water content Adsorption Animals Biophysical Phenomena Lecithins Membrane Lipids Membrane Potentials Membranes, Artificial Micelles Molecular Structure Phosphatidylethanolamines Pressure Proteins Spectroscopy, Fourier Transform Infrared Surface Properties Thermodynamics
description	The hydration of solid dimyristoylphosphatidylethanolamine (DMPE) produces a negligible shift in the asymmetric stretching frequency of the phosphate groups in contrast to dimyristoylphosphatidylcholine (DMPC). This suggests that the hydration of DMPE is not a consequence of the disruption of the solid lattice of the phosphate groups as occurs in DMPC. The strong lateral interactions between NH3 and PO2 - groups present in the solid PEs remain when the lipids are fully hydrated and seem to be a limiting factor for the hydration of the phosphate group hindering the reorientation of the polar heads. The lower mobility is reflected in a higher energy to translocate the phosphoethanolamine (P-N) dipoles in an electrical field. This energy is decreased in the presence of increasing ratios of PCs of saturated chains in phosphoethanolamine monolayer. The association of PC and PE in the membrane affecting the reorientation of the P-N groups is dependent of the chain-chain interaction. The dipole potentials of PCs and PEs mixtures show different behaviors according to the saturation of the acyl chain. This was correlated with the area in monolayers and the hydration of the P-N groups. In spite of the low hydration, DMPE is still able to adsorb fully hydrated proteins, although in a lower rate than DMPC at the same surface pressure. This indicates that PE interfaces posses an excess of surface free energy to drive protein interaction. The relation of this free energy with the low water content is discussed. © 2009 Elsevier B.V. All rights reserved.
format	JOUR
author	Bouchet, A.M. Frías, M.A. Lairion, F. Martini, F. Almaleck, H. Gordillo, G. Disalvo, E.A.
author_facet	Bouchet, A.M. Frías, M.A. Lairion, F. Martini, F. Almaleck, H. Gordillo, G. Disalvo, E.A.
author_sort	Bouchet, A.M.
title	Structural and dynamical surface properties of phosphatidylethanolamine containing membranes
title_short	Structural and dynamical surface properties of phosphatidylethanolamine containing membranes
title_full	Structural and dynamical surface properties of phosphatidylethanolamine containing membranes
title_fullStr	Structural and dynamical surface properties of phosphatidylethanolamine containing membranes
title_full_unstemmed	Structural and dynamical surface properties of phosphatidylethanolamine containing membranes
title_sort	structural and dynamical surface properties of phosphatidylethanolamine containing membranes
url	http://hdl.handle.net/20.500.12110/paper_00052736_v1788_n5_p918_Bouchet
work_keys_str_mv	AT bouchetam structuralanddynamicalsurfacepropertiesofphosphatidylethanolaminecontainingmembranes AT friasma structuralanddynamicalsurfacepropertiesofphosphatidylethanolaminecontainingmembranes AT lairionf structuralanddynamicalsurfacepropertiesofphosphatidylethanolaminecontainingmembranes AT martinif structuralanddynamicalsurfacepropertiesofphosphatidylethanolaminecontainingmembranes AT almaleckh structuralanddynamicalsurfacepropertiesofphosphatidylethanolaminecontainingmembranes AT gordillog structuralanddynamicalsurfacepropertiesofphosphatidylethanolaminecontainingmembranes AT disalvoea structuralanddynamicalsurfacepropertiesofphosphatidylethanolaminecontainingmembranes
_version_	1807318447140372480

Structural and dynamical surface properties of phosphatidylethanolamine containing membranes

Ejemplares similares