Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose

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A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species foun...

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Autores principales:	Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J.
Formato:	JOUR
Materias:	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Freeze Drying Phosphatidylethanolamines Trehalose Mammalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00052736_v1713_n1_p57_Ohtake
record_format	dspace
spelling	todo:paper_00052736_v1713_n1_p57_Ohtake2023-10-03T14:03:15Z Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose Ohtake, S. Schebor, C. Palecek, S.P. De Pablo, J.J. Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved. Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia
spellingShingle	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia Ohtake, S. Schebor, C. Palecek, S.P. De Pablo, J.J. Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
topic_facet	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia
description	A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.
format	JOUR
author	Ohtake, S. Schebor, C. Palecek, S.P. De Pablo, J.J.
author_facet	Ohtake, S. Schebor, C. Palecek, S.P. De Pablo, J.J.
author_sort	Ohtake, S.
title	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_short	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_full	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_fullStr	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_full_unstemmed	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_sort	phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
url	http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
work_keys_str_mv	AT ohtakes phasebehavioroffreezedriedphospholipidcholesterolmixturesstabilizedwithtrehalose AT scheborc phasebehavioroffreezedriedphospholipidcholesterolmixturesstabilizedwithtrehalose AT paleceksp phasebehavioroffreezedriedphospholipidcholesterolmixturesstabilizedwithtrehalose AT depablojj phasebehavioroffreezedriedphospholipidcholesterolmixturesstabilizedwithtrehalose
_version_	1782026633886040064

Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose

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