Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate
Native chloroplast fructose-1,6-bisphosphatase (d-fructose 1,6-bisphosphate-l-phosphohydrolase, EC 3.1.3.11) wasinactivated with either Woodward's reagent K or diethylpyrocarbonate. Since pseudo-first-order rate constants (κapp) were a hyperbolic function of inhibitor concentration, it appeared...
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todo:paper_00052728_v973_n3_p457_PratGay2023-10-03T14:03:14Z Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate Prat-Gay, G. Wolosiuk, RicardoA. Chaotropic anion Diethylpyrocarbonate: Thioredoxin Fructose-l,6-bisphosphalase: Woodward's reagent K Organic solvent Native chloroplast fructose-1,6-bisphosphatase (d-fructose 1,6-bisphosphate-l-phosphohydrolase, EC 3.1.3.11) wasinactivated with either Woodward's reagent K or diethylpyrocarbonate. Since pseudo-first-order rate constants (κapp) were a hyperbolic function of inhibitor concentration, it appeared that native chloroplast fructose-1,6-bisphosphatase and either Woodward's reagent K or diethylpyrocarbonate interacted reversibly prior to formation of irreversible (inactive) complex. Protection against inactivation was afforded by preincubating the enzyme with dithiothreitol, fructose 1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f or a chaotropic anion (trichloroacetate). Following similar incubation with an organic solvent (2-propanol), the catalytic activity remained unaltered in the presence of Woodward's reagent K but was inactivated by diethylpyrocarbonate. The enhancement of the specific activity of chloroplast fructose-1,6-bisphosphatase caused by dithiothreitol, fructose-1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f, or chaotropic anions or cosolvents was similar. Therefore, differential reactivity to selected reagents indicated the existence of several conformations of chloroplast fructose-1,6-bisphosphatase, i.e., native and different active forms. © 1989 Elsevier Science Publishers B.V. (Biomedical Division). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052728_v973_n3_p457_PratGay |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chaotropic anion Diethylpyrocarbonate: Thioredoxin Fructose-l,6-bisphosphalase: Woodward's reagent K Organic solvent |
| spellingShingle |
Chaotropic anion Diethylpyrocarbonate: Thioredoxin Fructose-l,6-bisphosphalase: Woodward's reagent K Organic solvent Prat-Gay, G. Wolosiuk, RicardoA. Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate |
| topic_facet |
Chaotropic anion Diethylpyrocarbonate: Thioredoxin Fructose-l,6-bisphosphalase: Woodward's reagent K Organic solvent |
| description |
Native chloroplast fructose-1,6-bisphosphatase (d-fructose 1,6-bisphosphate-l-phosphohydrolase, EC 3.1.3.11) wasinactivated with either Woodward's reagent K or diethylpyrocarbonate. Since pseudo-first-order rate constants (κapp) were a hyperbolic function of inhibitor concentration, it appeared that native chloroplast fructose-1,6-bisphosphatase and either Woodward's reagent K or diethylpyrocarbonate interacted reversibly prior to formation of irreversible (inactive) complex. Protection against inactivation was afforded by preincubating the enzyme with dithiothreitol, fructose 1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f or a chaotropic anion (trichloroacetate). Following similar incubation with an organic solvent (2-propanol), the catalytic activity remained unaltered in the presence of Woodward's reagent K but was inactivated by diethylpyrocarbonate. The enhancement of the specific activity of chloroplast fructose-1,6-bisphosphatase caused by dithiothreitol, fructose-1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f, or chaotropic anions or cosolvents was similar. Therefore, differential reactivity to selected reagents indicated the existence of several conformations of chloroplast fructose-1,6-bisphosphatase, i.e., native and different active forms. © 1989 Elsevier Science Publishers B.V. (Biomedical Division). |
| format |
JOUR |
| author |
Prat-Gay, G. Wolosiuk, RicardoA. |
| author_facet |
Prat-Gay, G. Wolosiuk, RicardoA. |
| author_sort |
Prat-Gay, G. |
| title |
Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate |
| title_short |
Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate |
| title_full |
Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate |
| title_fullStr |
Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate |
| title_full_unstemmed |
Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate |
| title_sort |
differential reactivity of chloroplast fructose-l,6-bisphosphatase to woodward's reagent k and diethylpyrocarbonate |
| url |
http://hdl.handle.net/20.500.12110/paper_00052728_v973_n3_p457_PratGay |
| work_keys_str_mv |
AT pratgayg differentialreactivityofchloroplastfructosel6bisphosphatasetowoodwardsreagentkanddiethylpyrocarbonate AT wolosiukricardoa differentialreactivityofchloroplastfructosel6bisphosphatasetowoodwardsreagentkanddiethylpyrocarbonate |
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