The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer
In this overview we present recent combined electrochemical, spectroelectrochemical, spectroscopic and computational studies from our group on the electron transfer reactions of cytochrome c and of the primary electron acceptor of cytochrome c oxidase, the CuA site, in biomimetic complexes. Based on...
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todo:paper_00052728_v1837_n7_p1196_AlvarezPaggi2023-10-03T14:03:14Z The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer Alvarez-Paggi, D. Zitare, U. Murgida, D.H. Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry azurin copper ion cytochrome c cytochrome c oxidase cytochrome c peroxidase hemoprotein histidine methionine plastocyanin porphyrin self assembled monolayer ubiquinol cytochrome c reductase algorithm biomimetics complex formation conceptual framework conference paper conformational transition crystal structure dipole electrochemistry electrode electron spin resonance electron transport energy transfer Escherichia coli ionic strength lipid bilayer molecular dynamics nonhuman nuclear magnetic resonance spectroscopy oxidation reduction potential oxidation reduction reaction oxidation reduction state pH photosystem I priority journal protein protein interaction protein secondary structure proton transport Raman spectrometry static electricity Thermus thermophilus Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry Amino Acid Sequence Animals Cytochromes c Electron Transport Electron Transport Complex IV Humans Molecular Docking Simulation Molecular Dynamics Simulation Molecular Sequence Data In this overview we present recent combined electrochemical, spectroelectrochemical, spectroscopic and computational studies from our group on the electron transfer reactions of cytochrome c and of the primary electron acceptor of cytochrome c oxidase, the CuA site, in biomimetic complexes. Based on these results, we discuss how protein dynamics and thermal fluctuations may impact on protein ET reactions, comment on the possible physiological relevance of these results, and finally propose a regulatory mechanism that may operate in the Cyt/CcO electron transfer reaction in vivo. This article is part of a Special Issue entitled: 18th European Bioenergetic Conference. © 2014 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052728_v1837_n7_p1196_AlvarezPaggi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry azurin copper ion cytochrome c cytochrome c oxidase cytochrome c peroxidase hemoprotein histidine methionine plastocyanin porphyrin self assembled monolayer ubiquinol cytochrome c reductase algorithm biomimetics complex formation conceptual framework conference paper conformational transition crystal structure dipole electrochemistry electrode electron spin resonance electron transport energy transfer Escherichia coli ionic strength lipid bilayer molecular dynamics nonhuman nuclear magnetic resonance spectroscopy oxidation reduction potential oxidation reduction reaction oxidation reduction state pH photosystem I priority journal protein protein interaction protein secondary structure proton transport Raman spectrometry static electricity Thermus thermophilus Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry Amino Acid Sequence Animals Cytochromes c Electron Transport Electron Transport Complex IV Humans Molecular Docking Simulation Molecular Dynamics Simulation Molecular Sequence Data |
| spellingShingle |
Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry azurin copper ion cytochrome c cytochrome c oxidase cytochrome c peroxidase hemoprotein histidine methionine plastocyanin porphyrin self assembled monolayer ubiquinol cytochrome c reductase algorithm biomimetics complex formation conceptual framework conference paper conformational transition crystal structure dipole electrochemistry electrode electron spin resonance electron transport energy transfer Escherichia coli ionic strength lipid bilayer molecular dynamics nonhuman nuclear magnetic resonance spectroscopy oxidation reduction potential oxidation reduction reaction oxidation reduction state pH photosystem I priority journal protein protein interaction protein secondary structure proton transport Raman spectrometry static electricity Thermus thermophilus Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry Amino Acid Sequence Animals Cytochromes c Electron Transport Electron Transport Complex IV Humans Molecular Docking Simulation Molecular Dynamics Simulation Molecular Sequence Data Alvarez-Paggi, D. Zitare, U. Murgida, D.H. The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer |
| topic_facet |
Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry azurin copper ion cytochrome c cytochrome c oxidase cytochrome c peroxidase hemoprotein histidine methionine plastocyanin porphyrin self assembled monolayer ubiquinol cytochrome c reductase algorithm biomimetics complex formation conceptual framework conference paper conformational transition crystal structure dipole electrochemistry electrode electron spin resonance electron transport energy transfer Escherichia coli ionic strength lipid bilayer molecular dynamics nonhuman nuclear magnetic resonance spectroscopy oxidation reduction potential oxidation reduction reaction oxidation reduction state pH photosystem I priority journal protein protein interaction protein secondary structure proton transport Raman spectrometry static electricity Thermus thermophilus Bioelectrochemistry CuA Cytochrome c Electron transfer Raman spectroelectrochemistry Amino Acid Sequence Animals Cytochromes c Electron Transport Electron Transport Complex IV Humans Molecular Docking Simulation Molecular Dynamics Simulation Molecular Sequence Data |
| description |
In this overview we present recent combined electrochemical, spectroelectrochemical, spectroscopic and computational studies from our group on the electron transfer reactions of cytochrome c and of the primary electron acceptor of cytochrome c oxidase, the CuA site, in biomimetic complexes. Based on these results, we discuss how protein dynamics and thermal fluctuations may impact on protein ET reactions, comment on the possible physiological relevance of these results, and finally propose a regulatory mechanism that may operate in the Cyt/CcO electron transfer reaction in vivo. This article is part of a Special Issue entitled: 18th European Bioenergetic Conference. © 2014 Elsevier B.V. |
| format |
JOUR |
| author |
Alvarez-Paggi, D. Zitare, U. Murgida, D.H. |
| author_facet |
Alvarez-Paggi, D. Zitare, U. Murgida, D.H. |
| author_sort |
Alvarez-Paggi, D. |
| title |
The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer |
| title_short |
The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer |
| title_full |
The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer |
| title_fullStr |
The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer |
| title_full_unstemmed |
The role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer |
| title_sort |
role of protein dynamics and thermal fluctuations in regulating cytochrome c/cytochrome c oxidase electron transfer |
| url |
http://hdl.handle.net/20.500.12110/paper_00052728_v1837_n7_p1196_AlvarezPaggi |
| work_keys_str_mv |
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1782029828318298112 |