Lipid-bound sugars in Rhizobium meliloti
Incubation of an enzyme preparation of Rhizobium meliloti with labeled uridine diphosphate glucose led to the formation of radioactive substances soluble in organic solvents. These substances are probably polyprenyl diphosphate saccharides. They behaved like these on treatment with ammonia or with h...
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todo:paper_00039861_v203_n1_p358_Tolmasky2023-10-03T13:56:54Z Lipid-bound sugars in Rhizobium meliloti Tolmasky, M.E. Staneloni, R.J. Ugalde, R.A. Leloir, L.F. disaccharide galactose glucosyltransferase isoprenoid phosphate sugar solabiose uridine diphosphate glucose article biosynthesis enzyme specificity enzymology kinetics metabolism paper chromatography Rhizobium temperature thin layer chromatography Chromatography, Paper Chromatography, Thin Layer Disaccharides Galactose Glucosyltransferases Kinetics Polyisoprenyl Phosphate Sugars Rhizobium Substrate Specificity Temperature Uridine Diphosphate Glucose Incubation of an enzyme preparation of Rhizobium meliloti with labeled uridine diphosphate glucose led to the formation of radioactive substances soluble in organic solvents. These substances are probably polyprenyl diphosphate saccharides. They behaved like these on treatment with ammonia or with hot phenol and were decomposed by heating for 10 min at pH 2 yielding a mono- and a disaccharide. The monosaccharide was identified as galactose by paper chromatography. The disaccharide gave glucose and galactose by acid hydrolysis. Following reduction with borohydride it yielded glucose and galactitol. After treatment with periodate followed by paper chromatography only galactose was detectable. The disaccharide was hydrolyzed by β- but not by α-glucosidase. Therefore the disaccharide is glucosyl β1-3-galactose. © 1980, All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00039861_v203_n1_p358_Tolmasky |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
disaccharide galactose glucosyltransferase isoprenoid phosphate sugar solabiose uridine diphosphate glucose article biosynthesis enzyme specificity enzymology kinetics metabolism paper chromatography Rhizobium temperature thin layer chromatography Chromatography, Paper Chromatography, Thin Layer Disaccharides Galactose Glucosyltransferases Kinetics Polyisoprenyl Phosphate Sugars Rhizobium Substrate Specificity Temperature Uridine Diphosphate Glucose |
| spellingShingle |
disaccharide galactose glucosyltransferase isoprenoid phosphate sugar solabiose uridine diphosphate glucose article biosynthesis enzyme specificity enzymology kinetics metabolism paper chromatography Rhizobium temperature thin layer chromatography Chromatography, Paper Chromatography, Thin Layer Disaccharides Galactose Glucosyltransferases Kinetics Polyisoprenyl Phosphate Sugars Rhizobium Substrate Specificity Temperature Uridine Diphosphate Glucose Tolmasky, M.E. Staneloni, R.J. Ugalde, R.A. Leloir, L.F. Lipid-bound sugars in Rhizobium meliloti |
| topic_facet |
disaccharide galactose glucosyltransferase isoprenoid phosphate sugar solabiose uridine diphosphate glucose article biosynthesis enzyme specificity enzymology kinetics metabolism paper chromatography Rhizobium temperature thin layer chromatography Chromatography, Paper Chromatography, Thin Layer Disaccharides Galactose Glucosyltransferases Kinetics Polyisoprenyl Phosphate Sugars Rhizobium Substrate Specificity Temperature Uridine Diphosphate Glucose |
| description |
Incubation of an enzyme preparation of Rhizobium meliloti with labeled uridine diphosphate glucose led to the formation of radioactive substances soluble in organic solvents. These substances are probably polyprenyl diphosphate saccharides. They behaved like these on treatment with ammonia or with hot phenol and were decomposed by heating for 10 min at pH 2 yielding a mono- and a disaccharide. The monosaccharide was identified as galactose by paper chromatography. The disaccharide gave glucose and galactose by acid hydrolysis. Following reduction with borohydride it yielded glucose and galactitol. After treatment with periodate followed by paper chromatography only galactose was detectable. The disaccharide was hydrolyzed by β- but not by α-glucosidase. Therefore the disaccharide is glucosyl β1-3-galactose. © 1980, All rights reserved. |
| format |
JOUR |
| author |
Tolmasky, M.E. Staneloni, R.J. Ugalde, R.A. Leloir, L.F. |
| author_facet |
Tolmasky, M.E. Staneloni, R.J. Ugalde, R.A. Leloir, L.F. |
| author_sort |
Tolmasky, M.E. |
| title |
Lipid-bound sugars in Rhizobium meliloti |
| title_short |
Lipid-bound sugars in Rhizobium meliloti |
| title_full |
Lipid-bound sugars in Rhizobium meliloti |
| title_fullStr |
Lipid-bound sugars in Rhizobium meliloti |
| title_full_unstemmed |
Lipid-bound sugars in Rhizobium meliloti |
| title_sort |
lipid-bound sugars in rhizobium meliloti |
| url |
http://hdl.handle.net/20.500.12110/paper_00039861_v203_n1_p358_Tolmasky |
| work_keys_str_mv |
AT tolmaskyme lipidboundsugarsinrhizobiummeliloti AT stanelonirj lipidboundsugarsinrhizobiummeliloti AT ugaldera lipidboundsugarsinrhizobiummeliloti AT leloirlf lipidboundsugarsinrhizobiummeliloti |
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1807320502280126464 |