Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
Nucleotides and sugar nucleotides from coelomic oocytes of Bufo arenarum were extracted with trichloroacetic acid and analyzed by ion-exchange chromatography. The hypoxanthine and guanine were sequencially eluted from the column with water. Nucleotides and sugar nucleotides were eluted with a linear...
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todo:paper_00039861_v180_n2_p269_Cantore2023-10-03T13:56:52Z Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes Cantore, M.L. De Recondo, M.E.F. Recondo, E.F. carbohydrate guanine hypoxanthine nucleotide radioisotope choline c 14 development egg in vitro study theoretical study Animal Bufo arenarum Choline Cytidine Diphosphate Choline Female Nucleoside Diphosphate Sugars Nucleotides Oocytes Oogenesis Ovum Pentoses Nucleotides and sugar nucleotides from coelomic oocytes of Bufo arenarum were extracted with trichloroacetic acid and analyzed by ion-exchange chromatography. The hypoxanthine and guanine were sequencially eluted from the column with water. Nucleotides and sugar nucleotides were eluted with a linear gradient of ammonium chloride. The first peak of ultraviolet adsorption eluted from the resin was a complex mixture of at least three substances. The main component was identified as cytidine diphosphocholine by chemical, enzymatic, and chromatographic analyses. Preliminary experiments suggest a possible role for this compound during oogenesis, since immature oocytes incubated in vitro with [14C]choline showed an active metabolism of this substance with rapid incorporation in choline phosphate, cytidine diphosphocholine, and lecithin. © 1977. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Recondo, E.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p269_Cantore |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carbohydrate guanine hypoxanthine nucleotide radioisotope choline c 14 development egg in vitro study theoretical study Animal Bufo arenarum Choline Cytidine Diphosphate Choline Female Nucleoside Diphosphate Sugars Nucleotides Oocytes Oogenesis Ovum Pentoses |
| spellingShingle |
carbohydrate guanine hypoxanthine nucleotide radioisotope choline c 14 development egg in vitro study theoretical study Animal Bufo arenarum Choline Cytidine Diphosphate Choline Female Nucleoside Diphosphate Sugars Nucleotides Oocytes Oogenesis Ovum Pentoses Cantore, M.L. De Recondo, M.E.F. Recondo, E.F. Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes |
| topic_facet |
carbohydrate guanine hypoxanthine nucleotide radioisotope choline c 14 development egg in vitro study theoretical study Animal Bufo arenarum Choline Cytidine Diphosphate Choline Female Nucleoside Diphosphate Sugars Nucleotides Oocytes Oogenesis Ovum Pentoses |
| description |
Nucleotides and sugar nucleotides from coelomic oocytes of Bufo arenarum were extracted with trichloroacetic acid and analyzed by ion-exchange chromatography. The hypoxanthine and guanine were sequencially eluted from the column with water. Nucleotides and sugar nucleotides were eluted with a linear gradient of ammonium chloride. The first peak of ultraviolet adsorption eluted from the resin was a complex mixture of at least three substances. The main component was identified as cytidine diphosphocholine by chemical, enzymatic, and chromatographic analyses. Preliminary experiments suggest a possible role for this compound during oogenesis, since immature oocytes incubated in vitro with [14C]choline showed an active metabolism of this substance with rapid incorporation in choline phosphate, cytidine diphosphocholine, and lecithin. © 1977. |
| format |
JOUR |
| author |
Cantore, M.L. De Recondo, M.E.F. Recondo, E.F. |
| author_facet |
Cantore, M.L. De Recondo, M.E.F. Recondo, E.F. |
| author_sort |
Cantore, M.L. |
| title |
Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes |
| title_short |
Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes |
| title_full |
Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes |
| title_fullStr |
Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes |
| title_full_unstemmed |
Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes |
| title_sort |
nucleotides and sugar nucleotides in early development of bufo arenarum. coelomic oocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p269_Cantore |
| work_keys_str_mv |
AT cantoreml nucleotidesandsugarnucleotidesinearlydevelopmentofbufoarenarumcoelomicoocytes AT derecondomef nucleotidesandsugarnucleotidesinearlydevelopmentofbufoarenarumcoelomicoocytes AT recondoef nucleotidesandsugarnucleotidesinearlydevelopmentofbufoarenarumcoelomicoocytes |
| _version_ |
1782027119350513664 |