Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis

Samples of peripheral blood were collected once from non-smoking women who were healthy (controls) and twice (immediately before and immediately after 7 days of treatment with metronidazole at 500 mg/day) from non-smoking women infected with Trichomonas vaginalis. Lymphocyte cultures were prepared a...

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Autores principales:	Carballo, M.A., Palermo, A.M., Mudry, M.D.
Formato:	JOUR
Materias:	metronidazole adult article blood sampling cell division clinical article clinical trial confidence interval controlled clinical trial controlled study drug effect drug screening female genotoxicity human human cell immune response lymphocyte culture lymphocyte proliferation mitosis index parameter sister chromatid exchange Trichomonas vaginalis trichomoniasis Adult Antitrichomonal Agents Cell Division Cells, Cultured Female Humans Lymphocytes Metronidazole Mitotic Index Sister Chromatid Exchange Trichomonas Vaginitis Protozoa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00034983_v98_n2_p139_Carballo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00034983_v98_n2_p139_Carballo2023-10-03T13:56:29Z Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis Carballo, M.A. Palermo, A.M. Mudry, M.D. metronidazole adult article blood sampling cell division clinical article clinical trial confidence interval controlled clinical trial controlled study drug effect drug screening female genotoxicity human human cell immune response lymphocyte culture lymphocyte proliferation mitosis index parameter sister chromatid exchange Trichomonas vaginalis trichomoniasis Adult Antitrichomonal Agents Cell Division Cells, Cultured Female Humans Lymphocytes Metronidazole Mitotic Index Sister Chromatid Exchange Trichomonas Vaginitis Protozoa Trichomonas vaginalis Samples of peripheral blood were collected once from non-smoking women who were healthy (controls) and twice (immediately before and immediately after 7 days of treatment with metronidazole at 500 mg/day) from non-smoking women infected with Trichomonas vaginalis. Lymphocyte cultures were prepared and used, in toxicogenetic studies, to determine the frequency of sister-chromatid exchange (SCE), the mitotic index (MI), and the replication index (RI) for each sample. MTZ treatment of the infected women led to an increase in the frequency of SCE (P < 0.001), a decrease in the MI (P < 0.003), and a modification in the kinetics of cell proliferation, with a decrease in the RI (P < 0.0006). The differences seen between the results for the controls and those for the infected women, before and after MTZ treatment, may be attributed to the presence of the parasite, to the treatment itself, and/or to variation in the host's response to infection with T. vaginalis. Fil:Palermo, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00034983_v98_n2_p139_Carballo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	metronidazole adult article blood sampling cell division clinical article clinical trial confidence interval controlled clinical trial controlled study drug effect drug screening female genotoxicity human human cell immune response lymphocyte culture lymphocyte proliferation mitosis index parameter sister chromatid exchange Trichomonas vaginalis trichomoniasis Adult Antitrichomonal Agents Cell Division Cells, Cultured Female Humans Lymphocytes Metronidazole Mitotic Index Sister Chromatid Exchange Trichomonas Vaginitis Protozoa Trichomonas vaginalis
spellingShingle	metronidazole adult article blood sampling cell division clinical article clinical trial confidence interval controlled clinical trial controlled study drug effect drug screening female genotoxicity human human cell immune response lymphocyte culture lymphocyte proliferation mitosis index parameter sister chromatid exchange Trichomonas vaginalis trichomoniasis Adult Antitrichomonal Agents Cell Division Cells, Cultured Female Humans Lymphocytes Metronidazole Mitotic Index Sister Chromatid Exchange Trichomonas Vaginitis Protozoa Trichomonas vaginalis Carballo, M.A. Palermo, A.M. Mudry, M.D. Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis
topic_facet	metronidazole adult article blood sampling cell division clinical article clinical trial confidence interval controlled clinical trial controlled study drug effect drug screening female genotoxicity human human cell immune response lymphocyte culture lymphocyte proliferation mitosis index parameter sister chromatid exchange Trichomonas vaginalis trichomoniasis Adult Antitrichomonal Agents Cell Division Cells, Cultured Female Humans Lymphocytes Metronidazole Mitotic Index Sister Chromatid Exchange Trichomonas Vaginitis Protozoa Trichomonas vaginalis
description	Samples of peripheral blood were collected once from non-smoking women who were healthy (controls) and twice (immediately before and immediately after 7 days of treatment with metronidazole at 500 mg/day) from non-smoking women infected with Trichomonas vaginalis. Lymphocyte cultures were prepared and used, in toxicogenetic studies, to determine the frequency of sister-chromatid exchange (SCE), the mitotic index (MI), and the replication index (RI) for each sample. MTZ treatment of the infected women led to an increase in the frequency of SCE (P < 0.001), a decrease in the MI (P < 0.003), and a modification in the kinetics of cell proliferation, with a decrease in the RI (P < 0.0006). The differences seen between the results for the controls and those for the infected women, before and after MTZ treatment, may be attributed to the presence of the parasite, to the treatment itself, and/or to variation in the host's response to infection with T. vaginalis.
format	JOUR
author	Carballo, M.A. Palermo, A.M. Mudry, M.D.
author_facet	Carballo, M.A. Palermo, A.M. Mudry, M.D.
author_sort	Carballo, M.A.
title	Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis
title_short	Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis
title_full	Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis
title_fullStr	Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis
title_full_unstemmed	Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis
title_sort	toxicogenetic evaluation of metronidazole in the treatment of women infected with trichomonas vaginalis
url	http://hdl.handle.net/20.500.12110/paper_00034983_v98_n2_p139_Carballo
work_keys_str_mv	AT carballoma toxicogeneticevaluationofmetronidazoleinthetreatmentofwomeninfectedwithtrichomonasvaginalis AT palermoam toxicogeneticevaluationofmetronidazoleinthetreatmentofwomeninfectedwithtrichomonasvaginalis AT mudrymd toxicogeneticevaluationofmetronidazoleinthetreatmentofwomeninfectedwithtrichomonasvaginalis
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Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis

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