Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to pr...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00032700_v81_n10_p3824_Pallarola |
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todo:paper_00032700_v81_n10_p3824_Pallarola2023-10-03T13:56:02Z Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay Pallarola, D. Battaglini, F. Amperometric Amphiphilic compound Aqueous medium Biological activities Electrode arrays Enzymatic activities Horseradish peroxidase HRP conjugate Hydrazide groups Lipopolysaccharide Lipopolysaccharides Simultaneous determinations Sodium deoxycholate Tetrafluoroborate Amines Hydrazine Sodium Surface active agents Polysaccharides 1 cyano 4 dimethylaminopyridinium tetrafluoroborate deoxycholate sodium lipopolysaccharide pyridinium derivative unclassified drug article bacterial strain chemical reaction chemical structure concentration (parameters) controlled study enzyme activity nonhuman pH measurement Binding, Competitive Dansyl Compounds Electrochemical Techniques Electrodes Horseradish Peroxidase Hydrazines Lipopolysaccharides Nitriles Pyridinium Compounds Surface-Active Agents The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. The method presents several advantages: it is carried out in a mild environment, good conjugation ratios are obtained, it is suitable for any label bearing amino, hydrazine, or hydrazide groups, and the LPS endotoxic and HRP enzymatic activities are preserved. The HRP conjugate is applied in an amperometric competitive assay for the detection of lipopolysaccharides in an electrode array combined with a multipotentiostat able to carry out simultaneous determinations. The system is able to detect samples in concentrations as low as 100 pg mL-1 of LPS. © 2009 American Chemical Society. Fil:Pallarola, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032700_v81_n10_p3824_Pallarola |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amperometric Amphiphilic compound Aqueous medium Biological activities Electrode arrays Enzymatic activities Horseradish peroxidase HRP conjugate Hydrazide groups Lipopolysaccharide Lipopolysaccharides Simultaneous determinations Sodium deoxycholate Tetrafluoroborate Amines Hydrazine Sodium Surface active agents Polysaccharides 1 cyano 4 dimethylaminopyridinium tetrafluoroborate deoxycholate sodium lipopolysaccharide pyridinium derivative unclassified drug article bacterial strain chemical reaction chemical structure concentration (parameters) controlled study enzyme activity nonhuman pH measurement Binding, Competitive Dansyl Compounds Electrochemical Techniques Electrodes Horseradish Peroxidase Hydrazines Lipopolysaccharides Nitriles Pyridinium Compounds Surface-Active Agents |
| spellingShingle |
Amperometric Amphiphilic compound Aqueous medium Biological activities Electrode arrays Enzymatic activities Horseradish peroxidase HRP conjugate Hydrazide groups Lipopolysaccharide Lipopolysaccharides Simultaneous determinations Sodium deoxycholate Tetrafluoroborate Amines Hydrazine Sodium Surface active agents Polysaccharides 1 cyano 4 dimethylaminopyridinium tetrafluoroborate deoxycholate sodium lipopolysaccharide pyridinium derivative unclassified drug article bacterial strain chemical reaction chemical structure concentration (parameters) controlled study enzyme activity nonhuman pH measurement Binding, Competitive Dansyl Compounds Electrochemical Techniques Electrodes Horseradish Peroxidase Hydrazines Lipopolysaccharides Nitriles Pyridinium Compounds Surface-Active Agents Pallarola, D. Battaglini, F. Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay |
| topic_facet |
Amperometric Amphiphilic compound Aqueous medium Biological activities Electrode arrays Enzymatic activities Horseradish peroxidase HRP conjugate Hydrazide groups Lipopolysaccharide Lipopolysaccharides Simultaneous determinations Sodium deoxycholate Tetrafluoroborate Amines Hydrazine Sodium Surface active agents Polysaccharides 1 cyano 4 dimethylaminopyridinium tetrafluoroborate deoxycholate sodium lipopolysaccharide pyridinium derivative unclassified drug article bacterial strain chemical reaction chemical structure concentration (parameters) controlled study enzyme activity nonhuman pH measurement Binding, Competitive Dansyl Compounds Electrochemical Techniques Electrodes Horseradish Peroxidase Hydrazines Lipopolysaccharides Nitriles Pyridinium Compounds Surface-Active Agents |
| description |
The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. The method presents several advantages: it is carried out in a mild environment, good conjugation ratios are obtained, it is suitable for any label bearing amino, hydrazine, or hydrazide groups, and the LPS endotoxic and HRP enzymatic activities are preserved. The HRP conjugate is applied in an amperometric competitive assay for the detection of lipopolysaccharides in an electrode array combined with a multipotentiostat able to carry out simultaneous determinations. The system is able to detect samples in concentrations as low as 100 pg mL-1 of LPS. © 2009 American Chemical Society. |
| format |
JOUR |
| author |
Pallarola, D. Battaglini, F. |
| author_facet |
Pallarola, D. Battaglini, F. |
| author_sort |
Pallarola, D. |
| title |
Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay |
| title_short |
Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay |
| title_full |
Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay |
| title_fullStr |
Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay |
| title_full_unstemmed |
Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay |
| title_sort |
surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay |
| url |
http://hdl.handle.net/20.500.12110/paper_00032700_v81_n10_p3824_Pallarola |
| work_keys_str_mv |
AT pallarolad surfactantassistedlipopolysaccharideconjugationemployingacyanopyridiniumagentanditsapplicationtoacompetitiveassay AT battaglinif surfactantassistedlipopolysaccharideconjugationemployingacyanopyridiniumagentanditsapplicationtoacompetitiveassay |
| _version_ |
1782025485106020352 |