Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique
A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic a...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00032697_v66_n2_p584_Krisman |
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todo:paper_00032697_v66_n2_p584_Krisman2023-10-03T13:55:55Z Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique Krisman, C.R. Barengo, R. carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. © 1975. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barengo, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032697_v66_n2_p584_Krisman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars |
| spellingShingle |
carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars Krisman, C.R. Barengo, R. Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| topic_facet |
carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars |
| description |
A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. © 1975. |
| format |
JOUR |
| author |
Krisman, C.R. Barengo, R. |
| author_facet |
Krisman, C.R. Barengo, R. |
| author_sort |
Krisman, C.R. |
| title |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_short |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_full |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_fullStr |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_full_unstemmed |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_sort |
assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| url |
http://hdl.handle.net/20.500.12110/paper_00032697_v66_n2_p584_Krisman |
| work_keys_str_mv |
AT krismancr assayofglucoproteinformationfromuridinediphosphateglucosebyarapidfilterpapertechnique AT barengor assayofglucoproteinformationfromuridinediphosphateglucosebyarapidfilterpapertechnique |
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