Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Optimization of bead analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after the screening of one-bead-one-peptide combinatorial libraries was achieved, involving the fine-tuning of the whole process. Guanidine was replaced by acetonitrile (MeCN)...

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Autores principales:	Martínez-Ceron, M.C., Giudicessi, S.L., Marani, M.M., Albericio, F., Cascone, O., Erra-Balsells, R., Camperi, S.A.
Formato:	JOUR
Materias:	acetic acid guanidine tetrahydrofuran trifluoroacetic acid urea ambient air article crystallization elution ionization matrix assisted laser desorption ionization time of flight mass spectrometry peptide analysis priority journal process optimization sensitivity analysis tandem mass spectrometry Acetic Acid Acetonitriles Hydroxides Peptide Library Peptides Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Water
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00032697_v400_n2_p295_MartinezCeron
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00032697_v400_n2_p295_MartinezCeron
record_format	dspace
spelling	todo:paper_00032697_v400_n2_p295_MartinezCeron2023-10-03T13:55:53Z Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Martínez-Ceron, M.C. Giudicessi, S.L. Marani, M.M. Albericio, F. Cascone, O. Erra-Balsells, R. Camperi, S.A. acetic acid guanidine tetrahydrofuran trifluoroacetic acid urea ambient air article crystallization elution ionization matrix assisted laser desorption ionization time of flight mass spectrometry peptide analysis priority journal process optimization sensitivity analysis tandem mass spectrometry Acetic Acid Acetonitriles Hydroxides Peptide Library Peptides Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Water Optimization of bead analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after the screening of one-bead-one-peptide combinatorial libraries was achieved, involving the fine-tuning of the whole process. Guanidine was replaced by acetonitrile (MeCN)/acetic acid (AcOH)/water (H2O), improving matrix crystallization. Peptide-bead cleavage with NH4OH was cheaper and safer than, yet as efficient as, NH3/tetrahydrofuran (THF). Peptide elution in microtubes instead of placing the beads in the sample plate yielded more sample aliquots. Successive dry layers deposit sample preparation was better than the dried droplet method. Among the matrices analyzed, α-cyano-4-hydroxycinnamic acid resulted in the best peptide ion yield. Cluster formation was minimized by the addition of additives to the matrix. © 2010 Elsevier Inc. Fil:Giudicessi, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032697_v400_n2_p295_MartinezCeron
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acetic acid guanidine tetrahydrofuran trifluoroacetic acid urea ambient air article crystallization elution ionization matrix assisted laser desorption ionization time of flight mass spectrometry peptide analysis priority journal process optimization sensitivity analysis tandem mass spectrometry Acetic Acid Acetonitriles Hydroxides Peptide Library Peptides Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Water
spellingShingle	acetic acid guanidine tetrahydrofuran trifluoroacetic acid urea ambient air article crystallization elution ionization matrix assisted laser desorption ionization time of flight mass spectrometry peptide analysis priority journal process optimization sensitivity analysis tandem mass spectrometry Acetic Acid Acetonitriles Hydroxides Peptide Library Peptides Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Water Martínez-Ceron, M.C. Giudicessi, S.L. Marani, M.M. Albericio, F. Cascone, O. Erra-Balsells, R. Camperi, S.A. Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
topic_facet	acetic acid guanidine tetrahydrofuran trifluoroacetic acid urea ambient air article crystallization elution ionization matrix assisted laser desorption ionization time of flight mass spectrometry peptide analysis priority journal process optimization sensitivity analysis tandem mass spectrometry Acetic Acid Acetonitriles Hydroxides Peptide Library Peptides Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Water
description	Optimization of bead analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after the screening of one-bead-one-peptide combinatorial libraries was achieved, involving the fine-tuning of the whole process. Guanidine was replaced by acetonitrile (MeCN)/acetic acid (AcOH)/water (H2O), improving matrix crystallization. Peptide-bead cleavage with NH4OH was cheaper and safer than, yet as efficient as, NH3/tetrahydrofuran (THF). Peptide elution in microtubes instead of placing the beads in the sample plate yielded more sample aliquots. Successive dry layers deposit sample preparation was better than the dried droplet method. Among the matrices analyzed, α-cyano-4-hydroxycinnamic acid resulted in the best peptide ion yield. Cluster formation was minimized by the addition of additives to the matrix. © 2010 Elsevier Inc.
format	JOUR
author	Martínez-Ceron, M.C. Giudicessi, S.L. Marani, M.M. Albericio, F. Cascone, O. Erra-Balsells, R. Camperi, S.A.
author_facet	Martínez-Ceron, M.C. Giudicessi, S.L. Marani, M.M. Albericio, F. Cascone, O. Erra-Balsells, R. Camperi, S.A.
author_sort	Martínez-Ceron, M.C.
title	Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_short	Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_full	Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_fullStr	Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_full_unstemmed	Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_sort	sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
url	http://hdl.handle.net/20.500.12110/paper_00032697_v400_n2_p295_MartinezCeron
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Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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