A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis

A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity pattern...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Krisman, C.R., Blumenfeld, M.L.
Formato: JOUR
Materias:
Glycogen synthase assay
glycogen synthase gel electrophoresis
glycogen synthase polyacrylamide gel detection
iodine staining
α-1,4 glucan synthase autoradiography
α-1,4 glucan synthase iodine staining
glycogen synthase
enzyme assay
methodology
nonhuman
polyacrylamide gel electrophoresis
priority journal
alpha-Amylase
Animal
Autoradiography
Electrophoresis, Polyacrylamide Gel
Glycogen Synthase
Iodine
Myocardium
Rats
Solutions
Staining and Labeling
Support, Non-U.S. Gov't
Time Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

Ejemplares similares

Ejemplares similares