A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis

A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity pattern...

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Autores principales: Krisman, C.R., Blumenfeld, M.L.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
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spelling todo:paper_00032697_v154_n2_p409_Krisman2023-10-03T13:55:47Z A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis Krisman, C.R. Blumenfeld, M.L. Glycogen synthase assay glycogen synthase gel electrophoresis glycogen synthase polyacrylamide gel detection iodine staining α-1,4 glucan synthase autoradiography α-1,4 glucan synthase iodine staining glycogen synthase enzyme assay methodology nonhuman polyacrylamide gel electrophoresis priority journal alpha-Amylase Animal Autoradiography Electrophoresis, Polyacrylamide Gel Glycogen Synthase Iodine Myocardium Rats Solutions Staining and Labeling Support, Non-U.S. Gov't Time Factors A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures. © 1986. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Blumenfeld, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Glycogen synthase assay
glycogen synthase gel electrophoresis
glycogen synthase polyacrylamide gel detection
iodine staining
α-1,4 glucan synthase autoradiography
α-1,4 glucan synthase iodine staining
glycogen synthase
enzyme assay
methodology
nonhuman
polyacrylamide gel electrophoresis
priority journal
alpha-Amylase
Animal
Autoradiography
Electrophoresis, Polyacrylamide Gel
Glycogen Synthase
Iodine
Myocardium
Rats
Solutions
Staining and Labeling
Support, Non-U.S. Gov't
Time Factors
spellingShingle Glycogen synthase assay
glycogen synthase gel electrophoresis
glycogen synthase polyacrylamide gel detection
iodine staining
α-1,4 glucan synthase autoradiography
α-1,4 glucan synthase iodine staining
glycogen synthase
enzyme assay
methodology
nonhuman
polyacrylamide gel electrophoresis
priority journal
alpha-Amylase
Animal
Autoradiography
Electrophoresis, Polyacrylamide Gel
Glycogen Synthase
Iodine
Myocardium
Rats
Solutions
Staining and Labeling
Support, Non-U.S. Gov't
Time Factors
Krisman, C.R.
Blumenfeld, M.L.
A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
topic_facet Glycogen synthase assay
glycogen synthase gel electrophoresis
glycogen synthase polyacrylamide gel detection
iodine staining
α-1,4 glucan synthase autoradiography
α-1,4 glucan synthase iodine staining
glycogen synthase
enzyme assay
methodology
nonhuman
polyacrylamide gel electrophoresis
priority journal
alpha-Amylase
Animal
Autoradiography
Electrophoresis, Polyacrylamide Gel
Glycogen Synthase
Iodine
Myocardium
Rats
Solutions
Staining and Labeling
Support, Non-U.S. Gov't
Time Factors
description A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures. © 1986.
format JOUR
author Krisman, C.R.
Blumenfeld, M.L.
author_facet Krisman, C.R.
Blumenfeld, M.L.
author_sort Krisman, C.R.
title A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_short A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_full A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_fullStr A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_full_unstemmed A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_sort method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
url http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
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AT krismancr methodforthedirectmeasurementofglycogensynthaseactivityongelsafterpolyacrylamidegelelectrophoresis
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