Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensi...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00027863_v128_n37_p12040_Spagnuolo |
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todo:paper_00027863_v128_n37_p12040_Spagnuolo2023-10-03T13:53:50Z Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins Spagnuolo, C.C. Vermeij, R.J. Jares-Erijman, E.A. arsenic derivative biotin cysteine derivative dye fluorescein absorption spectroscopy anisotropy article chemical structure complex formation fluorescence fluorescence resonance energy transfer fluorescence spectroscopy fluorination irradiation pH photochemistry Arsenicals Cysteine Drug Stability Fluoresceins Fluorescence Polarization Fluorescence Resonance Energy Transfer Hydrocarbons, Fluorinated Hydrogen-Ion Concentration Oligopeptides Photochemistry We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes. Copyright © 2006 American Chemical Society. Fil:Spagnuolo, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00027863_v128_n37_p12040_Spagnuolo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
arsenic derivative biotin cysteine derivative dye fluorescein absorption spectroscopy anisotropy article chemical structure complex formation fluorescence fluorescence resonance energy transfer fluorescence spectroscopy fluorination irradiation pH photochemistry Arsenicals Cysteine Drug Stability Fluoresceins Fluorescence Polarization Fluorescence Resonance Energy Transfer Hydrocarbons, Fluorinated Hydrogen-Ion Concentration Oligopeptides Photochemistry |
| spellingShingle |
arsenic derivative biotin cysteine derivative dye fluorescein absorption spectroscopy anisotropy article chemical structure complex formation fluorescence fluorescence resonance energy transfer fluorescence spectroscopy fluorination irradiation pH photochemistry Arsenicals Cysteine Drug Stability Fluoresceins Fluorescence Polarization Fluorescence Resonance Energy Transfer Hydrocarbons, Fluorinated Hydrogen-Ion Concentration Oligopeptides Photochemistry Spagnuolo, C.C. Vermeij, R.J. Jares-Erijman, E.A. Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins |
| topic_facet |
arsenic derivative biotin cysteine derivative dye fluorescein absorption spectroscopy anisotropy article chemical structure complex formation fluorescence fluorescence resonance energy transfer fluorescence spectroscopy fluorination irradiation pH photochemistry Arsenicals Cysteine Drug Stability Fluoresceins Fluorescence Polarization Fluorescence Resonance Energy Transfer Hydrocarbons, Fluorinated Hydrogen-Ion Concentration Oligopeptides Photochemistry |
| description |
We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes. Copyright © 2006 American Chemical Society. |
| format |
JOUR |
| author |
Spagnuolo, C.C. Vermeij, R.J. Jares-Erijman, E.A. |
| author_facet |
Spagnuolo, C.C. Vermeij, R.J. Jares-Erijman, E.A. |
| author_sort |
Spagnuolo, C.C. |
| title |
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins |
| title_short |
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins |
| title_full |
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins |
| title_fullStr |
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins |
| title_full_unstemmed |
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins |
| title_sort |
improved photostable fret-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins |
| url |
http://hdl.handle.net/20.500.12110/paper_00027863_v128_n37_p12040_Spagnuolo |
| work_keys_str_mv |
AT spagnuolocc improvedphotostablefretcompetentbiarsenicaltetracysteineprobesbasedonfluorinatedfluoresceins AT vermeijrj improvedphotostablefretcompetentbiarsenicaltetracysteineprobesbasedonfluorinatedfluoresceins AT jareserijmanea improvedphotostablefretcompetentbiarsenicaltetracysteineprobesbasedonfluorinatedfluoresceins |
| _version_ |
1782029372308324352 |