Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina

Flagellates indistinguishable from Trypanosoma cruzi were detected by microscopy in faecal samples of 2/110 Triatoma guasayana and 2/283 Triatoma garciabesi captured in a rural area of northwestern Argentina. Inoculation of faecal homogenates to mice followed by xenodiagnosis, haemoculture, histopat...

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Autores principales:	Schijman, A.G., Lauricella, M.A., Marcet, P.L., Duffy, T., Cardinal, M.V., Bisio, M., Levin, M.J., Kitron, U., Gürtler, R.E.
Formato:	JOUR
Lenguaje:	English
Materias:	24sα ribosomal RNA genes Blastocrithidia triatomae Chagas disease Triatoma garciabesi Triatoma guasayana Trypanosoma cruzi DNA 18S ribosome DNA fecal pellet flagellate histopathology inoculation insect microscopy phylogenetics polymerase chain reaction RNA rodent rural area amplicon animal experiment animal tissue Argentina article blastocrithidia triatomae blood culture Crithidia fasciculata disease carrier DNA sequence feces gene amplification gene sequence heart homogenate Leishmania major microorganism detection morphology mouse neighbor joining method nonhuman nucleotide sequence phylogenetic tree phylogeny protozoal infection RNA gene sample sequence analysis tissue culture Triatoma triatoma garciabesi triatoma guasayana Triatoma infestans Triatominae Trypanosoma trypanosomiasis xenodiagnosis Animals Base Sequence DNA, Kinetoplast Feces Insect Vectors Mice Mice, Inbred BALB C Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal Rural Population Sequence Alignment Sequence Analysis, DNA Trypanosomatina Xenodiagnosis South America Blastocrithidia triatomiae Mastigophora (flagellates) Trypanosomatidae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0001706X_v99_n1_p50_Schijman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0001706X_v99_n1_p50_Schijman2023-10-03T13:51:47Z Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina Schijman, A.G. Lauricella, M.A. Marcet, P.L. Duffy, T. Cardinal, M.V. Bisio, M. Levin, M.J. Kitron, U. Gürtler, R.E. 24sα ribosomal RNA genes Blastocrithidia triatomae Chagas disease Triatoma garciabesi Triatoma guasayana Trypanosoma cruzi DNA 18S ribosome DNA fecal pellet flagellate histopathology inoculation insect microscopy phylogenetics polymerase chain reaction RNA rodent rural area amplicon animal experiment animal tissue Argentina article blastocrithidia triatomae blood culture Crithidia fasciculata disease carrier DNA sequence feces flagellate gene amplification gene sequence heart histopathology homogenate inoculation Leishmania major microorganism detection microscopy morphology mouse neighbor joining method nonhuman nucleotide sequence phylogenetic tree phylogeny polymerase chain reaction protozoal infection RNA gene rural area sample sequence analysis tissue culture Triatoma triatoma garciabesi triatoma guasayana Triatoma infestans Triatominae Trypanosoma Trypanosoma cruzi trypanosomiasis xenodiagnosis Animals Argentina Base Sequence DNA, Kinetoplast Feces Insect Vectors Mice Mice, Inbred BALB C Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal Rural Population Sequence Alignment Sequence Analysis, DNA Triatoma Trypanosoma cruzi Trypanosomatina Xenodiagnosis Argentina South America Blastocrithidia triatomiae Mastigophora (flagellates) Triatoma garciabesi Triatoma guasayana Triatoma infestans Trypanosoma Trypanosoma cruzi Trypanosomatidae Flagellates indistinguishable from Trypanosoma cruzi were detected by microscopy in faecal samples of 2/110 Triatoma guasayana and 2/283 Triatoma garciabesi captured in a rural area of northwestern Argentina. Inoculation of faecal homogenates to mice followed by xenodiagnosis, haemoculture, histopathology and culture from cardiac homogenates, and PCR based on T. cruzi minicircle and nuclear sequences failed to detect T. cruzi infection, pointing to another trypanosomatidean. A PCR strategy targeted to the D7 domain of 24sα ribosomal DNA genes amplified a 250 bp sequence from one T. guasayana and one T. garciabesi faecal lysate. Sequence analysis revealed 100% identity with 24sα rDNA amplicons from Blastocrithidia triatomae obtained from faeces of reared Triatoma infestans bugs. Phylogenetic analysis clustered this sequence with C. fasciculata and L. major, separated from the Trypanosoma branch (bootstrap: 968/1000), in concordance with a Neighbour-joining dendrogram based on 18s rDNA sequences. This PCR procedure provides a rapid sensitive tool for differential diagnosis of morphologically similar trypanosomatids in field surveys of Chagas disease vectors and laboratory-reared triatomines used for xenodiagnosis. © 2006 Elsevier B.V. All rights reserved. Fil:Schijman, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marcet, P.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Duffy, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cardinal, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0001706X_v99_n1_p50_Schijman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	English
orig_language_str_mv	English
topic	24sα ribosomal RNA genes Blastocrithidia triatomae Chagas disease Triatoma garciabesi Triatoma guasayana Trypanosoma cruzi DNA 18S ribosome DNA fecal pellet flagellate histopathology inoculation insect microscopy phylogenetics polymerase chain reaction RNA rodent rural area amplicon animal experiment animal tissue Argentina article blastocrithidia triatomae blood culture Crithidia fasciculata disease carrier DNA sequence feces flagellate gene amplification gene sequence heart histopathology homogenate inoculation Leishmania major microorganism detection microscopy morphology mouse neighbor joining method nonhuman nucleotide sequence phylogenetic tree phylogeny polymerase chain reaction protozoal infection RNA gene rural area sample sequence analysis tissue culture Triatoma triatoma garciabesi triatoma guasayana Triatoma infestans Triatominae Trypanosoma Trypanosoma cruzi trypanosomiasis xenodiagnosis Animals Argentina Base Sequence DNA, Kinetoplast Feces Insect Vectors Mice Mice, Inbred BALB C Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal Rural Population Sequence Alignment Sequence Analysis, DNA Triatoma Trypanosoma cruzi Trypanosomatina Xenodiagnosis Argentina South America Blastocrithidia triatomiae Mastigophora (flagellates) Triatoma garciabesi Triatoma guasayana Triatoma infestans Trypanosoma Trypanosoma cruzi Trypanosomatidae
spellingShingle	24sα ribosomal RNA genes Blastocrithidia triatomae Chagas disease Triatoma garciabesi Triatoma guasayana Trypanosoma cruzi DNA 18S ribosome DNA fecal pellet flagellate histopathology inoculation insect microscopy phylogenetics polymerase chain reaction RNA rodent rural area amplicon animal experiment animal tissue Argentina article blastocrithidia triatomae blood culture Crithidia fasciculata disease carrier DNA sequence feces flagellate gene amplification gene sequence heart histopathology homogenate inoculation Leishmania major microorganism detection microscopy morphology mouse neighbor joining method nonhuman nucleotide sequence phylogenetic tree phylogeny polymerase chain reaction protozoal infection RNA gene rural area sample sequence analysis tissue culture Triatoma triatoma garciabesi triatoma guasayana Triatoma infestans Triatominae Trypanosoma Trypanosoma cruzi trypanosomiasis xenodiagnosis Animals Argentina Base Sequence DNA, Kinetoplast Feces Insect Vectors Mice Mice, Inbred BALB C Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal Rural Population Sequence Alignment Sequence Analysis, DNA Triatoma Trypanosoma cruzi Trypanosomatina Xenodiagnosis Argentina South America Blastocrithidia triatomiae Mastigophora (flagellates) Triatoma garciabesi Triatoma guasayana Triatoma infestans Trypanosoma Trypanosoma cruzi Trypanosomatidae Schijman, A.G. Lauricella, M.A. Marcet, P.L. Duffy, T. Cardinal, M.V. Bisio, M. Levin, M.J. Kitron, U. Gürtler, R.E. Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
topic_facet	24sα ribosomal RNA genes Blastocrithidia triatomae Chagas disease Triatoma garciabesi Triatoma guasayana Trypanosoma cruzi DNA 18S ribosome DNA fecal pellet flagellate histopathology inoculation insect microscopy phylogenetics polymerase chain reaction RNA rodent rural area amplicon animal experiment animal tissue Argentina article blastocrithidia triatomae blood culture Crithidia fasciculata disease carrier DNA sequence feces flagellate gene amplification gene sequence heart histopathology homogenate inoculation Leishmania major microorganism detection microscopy morphology mouse neighbor joining method nonhuman nucleotide sequence phylogenetic tree phylogeny polymerase chain reaction protozoal infection RNA gene rural area sample sequence analysis tissue culture Triatoma triatoma garciabesi triatoma guasayana Triatoma infestans Triatominae Trypanosoma Trypanosoma cruzi trypanosomiasis xenodiagnosis Animals Argentina Base Sequence DNA, Kinetoplast Feces Insect Vectors Mice Mice, Inbred BALB C Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal Rural Population Sequence Alignment Sequence Analysis, DNA Triatoma Trypanosoma cruzi Trypanosomatina Xenodiagnosis Argentina South America Blastocrithidia triatomiae Mastigophora (flagellates) Triatoma garciabesi Triatoma guasayana Triatoma infestans Trypanosoma Trypanosoma cruzi Trypanosomatidae
description	Flagellates indistinguishable from Trypanosoma cruzi were detected by microscopy in faecal samples of 2/110 Triatoma guasayana and 2/283 Triatoma garciabesi captured in a rural area of northwestern Argentina. Inoculation of faecal homogenates to mice followed by xenodiagnosis, haemoculture, histopathology and culture from cardiac homogenates, and PCR based on T. cruzi minicircle and nuclear sequences failed to detect T. cruzi infection, pointing to another trypanosomatidean. A PCR strategy targeted to the D7 domain of 24sα ribosomal DNA genes amplified a 250 bp sequence from one T. guasayana and one T. garciabesi faecal lysate. Sequence analysis revealed 100% identity with 24sα rDNA amplicons from Blastocrithidia triatomae obtained from faeces of reared Triatoma infestans bugs. Phylogenetic analysis clustered this sequence with C. fasciculata and L. major, separated from the Trypanosoma branch (bootstrap: 968/1000), in concordance with a Neighbour-joining dendrogram based on 18s rDNA sequences. This PCR procedure provides a rapid sensitive tool for differential diagnosis of morphologically similar trypanosomatids in field surveys of Chagas disease vectors and laboratory-reared triatomines used for xenodiagnosis. © 2006 Elsevier B.V. All rights reserved.
format	JOUR
author	Schijman, A.G. Lauricella, M.A. Marcet, P.L. Duffy, T. Cardinal, M.V. Bisio, M. Levin, M.J. Kitron, U. Gürtler, R.E.
author_facet	Schijman, A.G. Lauricella, M.A. Marcet, P.L. Duffy, T. Cardinal, M.V. Bisio, M. Levin, M.J. Kitron, U. Gürtler, R.E.
author_sort	Schijman, A.G.
title	Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
title_short	Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
title_full	Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
title_fullStr	Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
title_full_unstemmed	Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
title_sort	differential detection of blastocrithidia triatomae and trypanosoma cruzi by amplification of 24sα ribosomal rna genes in faeces of sylvatic triatomine species from rural northwestern argentina
url	http://hdl.handle.net/20.500.12110/paper_0001706X_v99_n1_p50_Schijman
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Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina

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