Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

Mostrar todas las versiones(2)

Fil: Romero Victorica, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.

Guardado en:
Detalles Bibliográficos
Autores principales: Romero Victorica, Matías, Soria, Marcelo Abel, Batista García, Ramón Alberto, Ceja Navarro, Javier A., Vikram, Surendra, Ortiz, Maximiliano, Ontañon, Ornella, Ghio, Silvina
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2020
Materias:
METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020romerovictorica
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2020romerovictorica
record_format dspace
spelling snrd:2020romerovictorica2021-10-15T16:56:07Z Romero Victorica, Matías Soria, Marcelo Abel Batista García, Ramón Alberto Ceja Navarro, Javier A. Vikram, Surendra Ortiz, Maximiliano Ontañon, Ornella Ghio, Silvina 2020 Fil: Romero Victorica, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina. Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina. Fil: Batista García, Ramón Alberto. Universidad Autónoma del Estado Morelos. Instituto de Investigación en Ciencias Básicas y Aplicadas. Centro de Investigación en Dinámica Celular. Cuernavaca, Morelos, Mexico. Fil: Ceja Navarro, Javier A. Biological Systems and Engineering Division. Lawrence Berkeley National Laboratory. Berkeley, California, USA. Fil: Vikram, Surendra. Department Biochemistry, Genetics and Microbiology. Centre for Microbial Ecology and Genomics. University of Pretoria. Pretoria, South Africa. Fil: Ortiz, Maximiliano.University of Pretoria. Centre for Microbial Ecology and Genomics. Genetics and Microbiology. Pretoria, South Africa. Fil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina. Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina. In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with diferent feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same fve dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with diferent relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifcally in genes coding for debranching- and oligosaccharide-degrading enzymes. These fndings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specifcity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50°C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications. tbls., grafs., il., fot. application/pdf 10.1038/s41598-020-60850-5 2045-2322 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020romerovictorica eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Scientific Reports Vol.10, no.3864 14 https://www.nature.com METAGENÓMICA SECUENCIACIÓN DE PRÓXIMA GENERACIÓN Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
spellingShingle METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
topic_facet METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
description Fil: Romero Victorica, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
author_facet Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
author_sort Romero Victorica, Matías
title Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_short Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_fullStr Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full_unstemmed Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_sort neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
publishDate 2020
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020romerovictorica
work_keys_str_mv AT romerovictoricamatias neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
AT soriamarceloabel neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
AT batistagarciaramonalberto neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
AT cejanavarrojaviera neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
AT vikramsurendra neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
AT ortizmaximiliano neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
AT ontanonornella neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
AT ghiosilvina neotropicaltermitemicrobiomesassourcesofnovelplantcellwalldegradingenzymes
_version_ 1790355020093325312

Ejemplares similares