Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type

Mostrar todas las versiones(2)

Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino (EEA Pergamino). Buenos Aires, Argentina.

Detalles Bibliográficos
Autores principales: González, Fernanda Gabriela, Capella, Matías, Ribichich, Karina Fabiana, Curín, Facundo, Giacomelli, Jorge Ignacio, Ayala, Francisco, Watson, Gerónimo, Otegui, María Elena, Chan, Raquel Lía
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2019
Materias:
DROUGHT TOLERANCE
GRAIN YIELD DETERMINATION
HAHB4
SUNFLOWER TRANSCRIPTION FACTOR
TRANSGENIC WHEAT
WATER USE EFFICIENCY
WHEAT FIELD TRIALS
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019gonzalez1
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2019gonzalez1
record_format dspace
spelling snrd:2019gonzalez12021-10-15T16:56:07Z González, Fernanda Gabriela Capella, Matías Ribichich, Karina Fabiana Curín, Facundo Giacomelli, Jorge Ignacio Ayala, Francisco Watson, Gerónimo Otegui, María Elena Chan, Raquel Lía 2019 Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino (EEA Pergamino). Buenos Aires, Argentina. Fil: Capella, Matías. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina. Fil: Ribichich, Karina Fabiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina. Fil: Curín, Facundo. Universidad Nacional del Noroeste de la provincia de Buenos Aires (UNNOBA). CITNOBA. Pergamino, Buenos Aires, Argentina. Fil: Giacomelli, Jorge Ignacio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina. Fil: Ayala, Francisco. INDEAR - BIOCERES. Rosario, Argentina. Fil: Watson, Gerónimo. INDEAR - BIOCERES. Rosario, Argentina. Fil: Otegui, María Elena. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina. Fil: Chan, Raquel Lía. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina. HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P smaller than 0.001) and had 9.4% larger water use efficiency (P smaller than 0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P smaller than 0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts. grafs., tbls., il., mapas, fot. application/pdf doi:10.1093/jxb/erz037 issn:0022-0957 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019gonzalez1 eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Journal of experimental botany Vol.70, no.5 1669–1681 https://academic.oup.com/jxb DROUGHT TOLERANCE GRAIN YIELD DETERMINATION HAHB4 SUNFLOWER TRANSCRIPTION FACTOR TRANSGENIC WHEAT WATER USE EFFICIENCY WHEAT FIELD TRIALS Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic DROUGHT TOLERANCE
GRAIN YIELD DETERMINATION
HAHB4
SUNFLOWER TRANSCRIPTION FACTOR
TRANSGENIC WHEAT
WATER USE EFFICIENCY
WHEAT FIELD TRIALS
spellingShingle DROUGHT TOLERANCE
GRAIN YIELD DETERMINATION
HAHB4
SUNFLOWER TRANSCRIPTION FACTOR
TRANSGENIC WHEAT
WATER USE EFFICIENCY
WHEAT FIELD TRIALS
González, Fernanda Gabriela
Capella, Matías
Ribichich, Karina Fabiana
Curín, Facundo
Giacomelli, Jorge Ignacio
Ayala, Francisco
Watson, Gerónimo
Otegui, María Elena
Chan, Raquel Lía
Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
topic_facet DROUGHT TOLERANCE
GRAIN YIELD DETERMINATION
HAHB4
SUNFLOWER TRANSCRIPTION FACTOR
TRANSGENIC WHEAT
WATER USE EFFICIENCY
WHEAT FIELD TRIALS
description Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino (EEA Pergamino). Buenos Aires, Argentina.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author González, Fernanda Gabriela
Capella, Matías
Ribichich, Karina Fabiana
Curín, Facundo
Giacomelli, Jorge Ignacio
Ayala, Francisco
Watson, Gerónimo
Otegui, María Elena
Chan, Raquel Lía
author_facet González, Fernanda Gabriela
Capella, Matías
Ribichich, Karina Fabiana
Curín, Facundo
Giacomelli, Jorge Ignacio
Ayala, Francisco
Watson, Gerónimo
Otegui, María Elena
Chan, Raquel Lía
author_sort González, Fernanda Gabriela
title Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_short Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_full Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_fullStr Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_full_unstemmed Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_sort field - grown transgenic wheat expressing the sunflower gene hahb4 significantly outyields the wild type
publishDate 2019
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019gonzalez1
work_keys_str_mv AT gonzalezfernandagabriela fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT capellamatias fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT ribichichkarinafabiana fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT curinfacundo fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT giacomellijorgeignacio fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT ayalafrancisco fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT watsongeronimo fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT oteguimariaelena fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
AT chanraquellia fieldgrowntransgenicwheatexpressingthesunflowergenehahb4significantlyoutyieldsthewildtype
_version_ 1827450705455087616

Ejemplares similares