Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)

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Fil: Ben Guerrero, Emiliano.Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina.

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Autores principales: Ben Guerrero, Emiliano, Soria, Marcelo Abel, Salvador, Ricardo, Ceja Navarro, Javier A., Campos, Eleonora, Brodie, Eoin L., Talia, Paola
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2016
Materias:
ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016guerrero
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
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spelling snrd:2016guerrero2021-10-15T16:56:07Z Ben Guerrero, Emiliano Soria, Marcelo Abel Salvador, Ricardo Ceja Navarro, Javier A. Campos, Eleonora Brodie, Eoin L. Talia, Paola 2016 Fil: Ben Guerrero, Emiliano.Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina. Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina. Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Centro Nacional de Investigaciones Agropecuarias. Instituto de Investigación Microbiología y Zoología Agrícola (IMyZA). Hurlingham, Buenos Aires, Argentina. Fil: Ceja Navarro, Javier A. Earth and Environmental Sciences. Lawrence Berkeley National Laboratory, Berkeley, CA, USA. Fil: Campos, Eleonora. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina. Fil: Brodie, Eoin L. Sciences. Lawrence Berkeley National Laboratory, Berkeley, CA, USA. Fil: Talia, Paola. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina. Cotton boll weevils, Anthonomus grandis, are omnivorous coleopteran that can feed on diets with different compositions, including recalcitrant lignocellulosic materials. We characterized the changes in the prokaryotic community structure and the hydrolytic activities of A. grandis larvae fed on different lignocellulosic diets. A. grandis larvae were fed on three different artificial diets: cottonseed meal (CM), Napier grass (NG) and corn stover (CS). Total DNA was extracted from the gut samples for amplification and sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Proteobacteria and Firmicutes dominated the gut microbiota followed by Actinobacteria, Spirochaetes and a small number of unclassified phyla in CM and NG microbiomes. In the CS feeding group, members of Spirochaetes were the most prevalent, followed by Proteobacteria and Firmicutes. Bray–Curtis distances showed that the samples from the CS community were clearly separated from those samples of the CM and NG diets. Gut extracts from all three diets exhibited endoglucanase, xylanase, ß-glucosidase and pectinase activities. These activities were significantly affected by pH and temperature across different diets. We observed that the larvae reared on a CM showed significantly higher activities than larvae reared on NG and CS. We demonstrated that the intestinal bacterial community structure varies depending on diet composition. Diets with more variable and complex compositions, such as CS, showed higher bacterial diversity and richness than the two other diets. In spite of the detected changes in composition and diversity, we identified a core microbiome shared between the three different lignocellulosic diets. These results suggest that feeding with diets of different lignocellulosic composition could be a viable strategy to discover variants of hemicellulose and cellulose breakdown systems. grafs., tbls. application/pdf 10.3389/fmicb.2016.02093 1664-302X http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016guerrero eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Frontiers in Microbiology Vol.7 e2093,123p. https://www.frontiersin.org/ ANTHONOMUS GRANDIS GUT MICROBIOTA 16S RRNA GENE ILLUMINA AMPLICON SEQUENCING HYDROLYTIC ACTIVITIES LIGNOCELLULOSIC FEEDSTOCKS Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis) info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
spellingShingle ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
Ben Guerrero, Emiliano
Soria, Marcelo Abel
Salvador, Ricardo
Ceja Navarro, Javier A.
Campos, Eleonora
Brodie, Eoin L.
Talia, Paola
Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
topic_facet ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
description Fil: Ben Guerrero, Emiliano.Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina.
format Artículo
Artículo
publishedVersion
publishedVersion
author Ben Guerrero, Emiliano
Soria, Marcelo Abel
Salvador, Ricardo
Ceja Navarro, Javier A.
Campos, Eleonora
Brodie, Eoin L.
Talia, Paola
author_facet Ben Guerrero, Emiliano
Soria, Marcelo Abel
Salvador, Ricardo
Ceja Navarro, Javier A.
Campos, Eleonora
Brodie, Eoin L.
Talia, Paola
author_sort Ben Guerrero, Emiliano
title Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_short Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_full Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_fullStr Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_full_unstemmed Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_sort effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (anthonomus grandis)
publishDate 2016
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016guerrero
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