Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus

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Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.

Detalles Bibliográficos
Autores principales: Santo Domingo, Cinthia, Soria, Marcelo Abel, Rojas, Ana M., Fissore, Eliana N., Gerschenson, Lia N.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2015
Materias:
URONIC ACID
SPECTROPHOTOMETRY
PROTEINASE
PROTEIN ANALYSIS
PROTEIN
PROTEASE
POLYPHENOLS
POLYPHENOL
PECTIN
NONHUMAN
INULIN
HYDROLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
HEMICELLULOSE
HEMICELLULASE
GALACTURONIC ACID
FLOW RATE
ENZYME ISOLATION
ENZYME ACTIVITY
ELECTROSPRAY MASS SPECTROMETRY
DIETARY FIBER
CYNARA CARDUNCULUS
CONTROLLED STUDY
CHEMICAL COMPOSITION
CARDOON
BIOMASS TO CHEMICALS
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2015santodomingo
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2015santodomingo
record_format dspace
spelling snrd:2015santodomingo2021-10-15T16:56:07Z Santo Domingo, Cinthia Soria, Marcelo Abel Rojas, Ana M. Fissore, Eliana N. Gerschenson, Lia N. 2015 Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. Fil: Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0-200 ?L and hemicellulase amounts of 0-200 mg for 5 g of material, we explored the effect of a 5 h enzymatic treatment at 40 °C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol. application/pdf doi:10.3390/ijms16036057 issn:1661-6596 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2015santodomingo eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 International Journal of Molecular Sciences Vol.16, no.3 6057-6075 http://www.mdpi.com/journal/ijms URONIC ACID SPECTROPHOTOMETRY PROTEINASE PROTEIN ANALYSIS PROTEIN PROTEASE POLYPHENOLS POLYPHENOL PECTIN NONHUMAN INULIN HYDROLYSIS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY HEMICELLULOSE HEMICELLULASE GALACTURONIC ACID FLOW RATE ENZYME ISOLATION ENZYME ACTIVITY ELECTROSPRAY MASS SPECTROMETRY DIETARY FIBER CYNARA CARDUNCULUS CONTROLLED STUDY CHEMICAL COMPOSITION CARDOON BIOMASS TO CHEMICALS Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic URONIC ACID
SPECTROPHOTOMETRY
PROTEINASE
PROTEIN ANALYSIS
PROTEIN
PROTEASE
POLYPHENOLS
POLYPHENOL
PECTIN
NONHUMAN
INULIN
HYDROLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
HEMICELLULOSE
HEMICELLULASE
GALACTURONIC ACID
FLOW RATE
ENZYME ISOLATION
ENZYME ACTIVITY
ELECTROSPRAY MASS SPECTROMETRY
DIETARY FIBER
CYNARA CARDUNCULUS
CONTROLLED STUDY
CHEMICAL COMPOSITION
CARDOON
BIOMASS TO CHEMICALS
spellingShingle URONIC ACID
SPECTROPHOTOMETRY
PROTEINASE
PROTEIN ANALYSIS
PROTEIN
PROTEASE
POLYPHENOLS
POLYPHENOL
PECTIN
NONHUMAN
INULIN
HYDROLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
HEMICELLULOSE
HEMICELLULASE
GALACTURONIC ACID
FLOW RATE
ENZYME ISOLATION
ENZYME ACTIVITY
ELECTROSPRAY MASS SPECTROMETRY
DIETARY FIBER
CYNARA CARDUNCULUS
CONTROLLED STUDY
CHEMICAL COMPOSITION
CARDOON
BIOMASS TO CHEMICALS
Santo Domingo, Cinthia
Soria, Marcelo Abel
Rojas, Ana M.
Fissore, Eliana N.
Gerschenson, Lia N.
Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
topic_facet URONIC ACID
SPECTROPHOTOMETRY
PROTEINASE
PROTEIN ANALYSIS
PROTEIN
PROTEASE
POLYPHENOLS
POLYPHENOL
PECTIN
NONHUMAN
INULIN
HYDROLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
HEMICELLULOSE
HEMICELLULASE
GALACTURONIC ACID
FLOW RATE
ENZYME ISOLATION
ENZYME ACTIVITY
ELECTROSPRAY MASS SPECTROMETRY
DIETARY FIBER
CYNARA CARDUNCULUS
CONTROLLED STUDY
CHEMICAL COMPOSITION
CARDOON
BIOMASS TO CHEMICALS
description Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
format Artículo
Artículo
publishedVersion
publishedVersion
author Santo Domingo, Cinthia
Soria, Marcelo Abel
Rojas, Ana M.
Fissore, Eliana N.
Gerschenson, Lia N.
author_facet Santo Domingo, Cinthia
Soria, Marcelo Abel
Rojas, Ana M.
Fissore, Eliana N.
Gerschenson, Lia N.
author_sort Santo Domingo, Cinthia
title Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
title_short Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
title_full Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
title_fullStr Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
title_full_unstemmed Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
title_sort protease and hemicellulase assisted extraction of dietary fiber from wastes of cynara cardunculus
publishDate 2015
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2015santodomingo
work_keys_str_mv AT santodomingocinthia proteaseandhemicellulaseassistedextractionofdietaryfiberfromwastesofcynaracardunculus
AT soriamarceloabel proteaseandhemicellulaseassistedextractionofdietaryfiberfromwastesofcynaracardunculus
AT rojasanam proteaseandhemicellulaseassistedextractionofdietaryfiberfromwastesofcynaracardunculus
AT fissoreelianan proteaseandhemicellulaseassistedextractionofdietaryfiberfromwastesofcynaracardunculus
AT gerschensonlian proteaseandhemicellulaseassistedextractionofdietaryfiberfromwastesofcynaracardunculus
_version_ 1827450611854999552

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