In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy

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Fil: Cantoro, Renata. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.

Detalles Bibliográficos
Autores principales: Cantoro, Renata, Crocco, Carlos Daniel, Benech Arnold, Roberto Luis, Rodríguez, María Verónica
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2013
Materias:
ABI4
ABI5
ABSCISIC ACID
GA 2-OXIDASE
GERMINATION
GIBBERELLINS
SEED DORMANCY
SORGHUM BICOLOR
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2013cantoro
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2013cantoro
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic ABI4
ABI5
ABSCISIC ACID
GA 2-OXIDASE
GERMINATION
GIBBERELLINS
SEED DORMANCY
SORGHUM BICOLOR
spellingShingle ABI4
ABI5
ABSCISIC ACID
GA 2-OXIDASE
GERMINATION
GIBBERELLINS
SEED DORMANCY
SORGHUM BICOLOR
Cantoro, Renata
Crocco, Carlos Daniel
Benech Arnold, Roberto Luis
Rodríguez, María Verónica
In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy
topic_facet ABI4
ABI5
ABSCISIC ACID
GA 2-OXIDASE
GERMINATION
GIBBERELLINS
SEED DORMANCY
SORGHUM BICOLOR
description Fil: Cantoro, Renata. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author Cantoro, Renata
Crocco, Carlos Daniel
Benech Arnold, Roberto Luis
Rodríguez, María Verónica
author_facet Cantoro, Renata
Crocco, Carlos Daniel
Benech Arnold, Roberto Luis
Rodríguez, María Verónica
author_sort Cantoro, Renata
title In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy
title_short In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy
title_full In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy
title_fullStr In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy
title_full_unstemmed In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy
title_sort in vitro binding of sorghum bicolor transcription factors abi4 and abi5 to a conserved region of a ga 2-oxidase promoter : possible role of this interaction in the expression of seed dormancy
publishDate 2013
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2013cantoro
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AT croccocarlosdaniel invitrobindingofsorghumbicolortranscriptionfactorsabi4andabi5toaconservedregionofaga2oxidasepromoterpossibleroleofthisinteractionintheexpressionofseeddormancy
AT benecharnoldrobertoluis invitrobindingofsorghumbicolortranscriptionfactorsabi4andabi5toaconservedregionofaga2oxidasepromoterpossibleroleofthisinteractionintheexpressionofseeddormancy
AT rodriguezmariaveronica invitrobindingofsorghumbicolortranscriptionfactorsabi4andabi5toaconservedregionofaga2oxidasepromoterpossibleroleofthisinteractionintheexpressionofseeddormancy
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spelling snrd:2013cantoro2021-10-15T16:56:07Z Cantoro, Renata Crocco, Carlos Daniel Benech Arnold, Roberto Luis Rodríguez, María Verónica 2013 Fil: Cantoro, Renata. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Cantoro, Renata. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Cantoro, Renata. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cultivos Industriales. Buenos Aires, Argentina. Fil: Crocco, Carlos Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Crocco, Carlos Daniel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Benech Arnold, Roberto Luis. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Benech Arnold, Roberto Luis. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Benech Arnold, Roberto Luis. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cultivos Industriales. Buenos Aires, Argentina. Fil: Rodríguez, María Verónica. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Rodríguez, María Verónica. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. The precise adjustment of the timing of dormancy release according to final grain usage is still a challenge for many cereal crops. Grain sorghum (Sorghum bicolor (L.) Moench) shows wide intraspecific variability in dormancy level and susceptibility to pre-harvest sprouting (PHS). Both embryo sensitivity to abscisic acid (ABA) and gibberellin (GA) metabolism play an important role in the expression of dormancy of the developing sorghum grain. In previous works, it was shown that, simultaneously with a greater embryo sensitivity to ABA and higher expression of SbABA-INSENSITIVE 4 (SbABI4) and SbABA-INSENSITIVE 5 (SbABI5), dormant grains accumulate less active GA4 due to a more active GA catabolism. In this work, it is demonstrated that the ABA signalling components SbABI4 and SbABI5 interact in vitro with a fragment of the SbGA 2-OXIDASE 3 (SbGA2ox3) promoter containing an ABA-responsive complex (ABRC). Both transcription factors were able to bind the promoter, although not simultaneously, suggesting that they might compete for the same cis-acting regulatory sequences. A biological role for these interactions in the expression of dormancy of sorghum grains is proposed: either SbABI4 and/or SbABI5 activate transcription of the SbGA2ox3 gene in vivo and promote SbGA2ox3 protein accumulation; this would result in active degradation of GA4, thus preventing germination of dormant grains. A comparative analysis of the 5'-regulatory region of GA2oxs from both monocots and dicots is also presented; conservation of the ABRC in closely related GA2oxs from Brachypodium distachyon and rice suggest that these species might share the same regulatory mechanism as proposed for grain sorghum. application/pdf doi:10.1093/jxb/ert347 issn:0022-0957 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2013cantoro eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Journal of Experimental Botany Vol.64, no.18 5721-5735 https://academic.oup.com/jxb ABI4 ABI5 ABSCISIC ACID GA 2-OXIDASE GERMINATION GIBBERELLINS SEED DORMANCY SORGHUM BICOLOR In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter : Possible role of this interaction in the expression of seed dormancy article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion

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