Bovine parthenogenotes produced by inhibition of first or second polar bodies emission

Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion o...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Bevacqua, R. J., Fernandez-Martin, R., Salamone, D. F.
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2011
Materias:
CYTOCHALASIN B
MEIOTIC MATURATION
MICROFILAMENTS
PARTHENOGENESIS
ACTIN
ANIMAL CELL
ANIMAL TISSUE
CELL ACTIVATION
CELL CULTURE
CELL CYCLE ARREST
CELL CYCLE PARAMETERS
CONTROLLED STUDY
DIPLOIDY
EMBRYO
EMBRYO DEVELOPMENT
EMBRYO INDUCTION
GROWTH RATE
IN VITRO STUDY
MEIOSIS
METAPHASE
MICROFILAMENT
NONHUMAN
OOCYTE
OOCYTE MATURATION
POLAR BODY
BOVINAE
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Bevacqua
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2011Bevacqua
record_format dspace
spelling snrd:2011Bevacqua2021-10-15T16:56:07Z Bevacqua, R. J. Fernandez-Martin, R. Salamone, D. F. 2011 Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77 percent) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor. application/pdf 0327-9545 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Bevacqua eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Biocell Vol.35, no.1 1-7 http://www.cricyt.edu.ar/ CYTOCHALASIN B MEIOTIC MATURATION MICROFILAMENTS PARTHENOGENESIS ACTIN ANIMAL CELL ANIMAL TISSUE CELL ACTIVATION CELL CULTURE CELL CYCLE ARREST CELL CYCLE PARAMETERS CONTROLLED STUDY DIPLOIDY EMBRYO EMBRYO DEVELOPMENT EMBRYO INDUCTION GROWTH RATE IN VITRO STUDY MEIOSIS METAPHASE MICROFILAMENT NONHUMAN OOCYTE OOCYTE MATURATION PARTHENOGENESIS POLAR BODY BOVINAE Bovine parthenogenotes produced by inhibition of first or second polar bodies emission article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic CYTOCHALASIN B
MEIOTIC MATURATION
MICROFILAMENTS
PARTHENOGENESIS
ACTIN
ANIMAL CELL
ANIMAL TISSUE
CELL ACTIVATION
CELL CULTURE
CELL CYCLE ARREST
CELL CYCLE PARAMETERS
CONTROLLED STUDY
DIPLOIDY
EMBRYO
EMBRYO DEVELOPMENT
EMBRYO INDUCTION
GROWTH RATE
IN VITRO STUDY
MEIOSIS
METAPHASE
MICROFILAMENT
NONHUMAN
OOCYTE
OOCYTE MATURATION
PARTHENOGENESIS
POLAR BODY
BOVINAE
spellingShingle CYTOCHALASIN B
MEIOTIC MATURATION
MICROFILAMENTS
PARTHENOGENESIS
ACTIN
ANIMAL CELL
ANIMAL TISSUE
CELL ACTIVATION
CELL CULTURE
CELL CYCLE ARREST
CELL CYCLE PARAMETERS
CONTROLLED STUDY
DIPLOIDY
EMBRYO
EMBRYO DEVELOPMENT
EMBRYO INDUCTION
GROWTH RATE
IN VITRO STUDY
MEIOSIS
METAPHASE
MICROFILAMENT
NONHUMAN
OOCYTE
OOCYTE MATURATION
PARTHENOGENESIS
POLAR BODY
BOVINAE
Bevacqua, R. J.
Fernandez-Martin, R.
Salamone, D. F.
Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
topic_facet CYTOCHALASIN B
MEIOTIC MATURATION
MICROFILAMENTS
PARTHENOGENESIS
ACTIN
ANIMAL CELL
ANIMAL TISSUE
CELL ACTIVATION
CELL CULTURE
CELL CYCLE ARREST
CELL CYCLE PARAMETERS
CONTROLLED STUDY
DIPLOIDY
EMBRYO
EMBRYO DEVELOPMENT
EMBRYO INDUCTION
GROWTH RATE
IN VITRO STUDY
MEIOSIS
METAPHASE
MICROFILAMENT
NONHUMAN
OOCYTE
OOCYTE MATURATION
PARTHENOGENESIS
POLAR BODY
BOVINAE
description Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77 percent) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author Bevacqua, R. J.
Fernandez-Martin, R.
Salamone, D. F.
author_facet Bevacqua, R. J.
Fernandez-Martin, R.
Salamone, D. F.
author_sort Bevacqua, R. J.
title Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
title_short Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
title_full Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
title_fullStr Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
title_full_unstemmed Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
title_sort bovine parthenogenotes produced by inhibition of first or second polar bodies emission
publishDate 2011
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Bevacqua
work_keys_str_mv AT bevacquarj bovineparthenogenotesproducedbyinhibitionoffirstorsecondpolarbodiesemission
AT fernandezmartinr bovineparthenogenotesproducedbyinhibitionoffirstorsecondpolarbodiesemission
AT salamonedf bovineparthenogenotesproducedbyinhibitionoffirstorsecondpolarbodiesemission
_version_ 1790354991478734848

Ejemplares similares