The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site

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It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present wor...

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Detalles Bibliográficos
Autores principales: Álvarez, Y.D., Belingheri, A.V., Perez Bay, A.E., Javis, S.E., Tedford, H.W., Zamponi, G., Marengo, F.D.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2013
Materias:
calcium channel P type
calcium channel Q type
omega agatoxin IVA
potassium ion
animal cell
article
binding site
calcium current
cell interaction
cell vacuole
chromaffin cell
controlled study
depolarization
exocytosis
mouse
nerve cell stimulation
nonhuman
protein expression
protein interaction
sequence analysis
synapse
transient expression
Animals
Calcium
Calcium Channels, P-Type
Calcium Channels, Q-Type
Cells, Cultured
Chromaffin Cells
Exocytosis
Mice
Secretory Vesicles
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19326203_v8_n1_p_Alvarez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_19326203_v8_n1_p_Alvarez
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spelling paperaa:paper_19326203_v8_n1_p_Alvarez2023-06-12T16:51:44Z The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site PLoS ONE 2013;8(1) Álvarez, Y.D. Belingheri, A.V. Perez Bay, A.E. Javis, S.E. Tedford, H.W. Zamponi, G. Marengo, F.D. calcium channel P type calcium channel Q type omega agatoxin IVA potassium ion animal cell article binding site calcium current cell interaction cell vacuole chromaffin cell controlled study depolarization exocytosis mouse nerve cell stimulation nonhuman protein expression protein interaction sequence analysis synapse transient expression Animals Calcium Calcium Channels, P-Type Calcium Channels, Q-Type Cells, Cultured Chromaffin Cells Exocytosis Mice Secretory Vesicles It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present work we found that the Ca2+ current flowing through P/Q-type Ca2+ channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca2+ current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K+ stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca2+ channels. © 2013 Álvarez et al. Fil:Álvarez, Y.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Belingheri, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perez Bay, A.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marengo, F.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v8_n1_p_Alvarez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic calcium channel P type
calcium channel Q type
omega agatoxin IVA
potassium ion
animal cell
article
binding site
calcium current
cell interaction
cell vacuole
chromaffin cell
controlled study
depolarization
exocytosis
mouse
nerve cell stimulation
nonhuman
protein expression
protein interaction
sequence analysis
synapse
transient expression
Animals
Calcium
Calcium Channels, P-Type
Calcium Channels, Q-Type
Cells, Cultured
Chromaffin Cells
Exocytosis
Mice
Secretory Vesicles
spellingShingle calcium channel P type
calcium channel Q type
omega agatoxin IVA
potassium ion
animal cell
article
binding site
calcium current
cell interaction
cell vacuole
chromaffin cell
controlled study
depolarization
exocytosis
mouse
nerve cell stimulation
nonhuman
protein expression
protein interaction
sequence analysis
synapse
transient expression
Animals
Calcium
Calcium Channels, P-Type
Calcium Channels, Q-Type
Cells, Cultured
Chromaffin Cells
Exocytosis
Mice
Secretory Vesicles
Álvarez, Y.D.
Belingheri, A.V.
Perez Bay, A.E.
Javis, S.E.
Tedford, H.W.
Zamponi, G.
Marengo, F.D.
The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
topic_facet calcium channel P type
calcium channel Q type
omega agatoxin IVA
potassium ion
animal cell
article
binding site
calcium current
cell interaction
cell vacuole
chromaffin cell
controlled study
depolarization
exocytosis
mouse
nerve cell stimulation
nonhuman
protein expression
protein interaction
sequence analysis
synapse
transient expression
Animals
Calcium
Calcium Channels, P-Type
Calcium Channels, Q-Type
Cells, Cultured
Chromaffin Cells
Exocytosis
Mice
Secretory Vesicles
description It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present work we found that the Ca2+ current flowing through P/Q-type Ca2+ channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca2+ current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K+ stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca2+ channels. © 2013 Álvarez et al.
format Artículo
Artículo
publishedVersion
author Álvarez, Y.D.
Belingheri, A.V.
Perez Bay, A.E.
Javis, S.E.
Tedford, H.W.
Zamponi, G.
Marengo, F.D.
author_facet Álvarez, Y.D.
Belingheri, A.V.
Perez Bay, A.E.
Javis, S.E.
Tedford, H.W.
Zamponi, G.
Marengo, F.D.
author_sort Álvarez, Y.D.
title The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
title_short The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
title_full The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
title_fullStr The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
title_full_unstemmed The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
title_sort immediately releasable pool of mouse chromaffin cell vesicles is coupled to p/q-type calcium channels via the synaptic protein interaction site
publishDate 2013
url http://hdl.handle.net/20.500.12110/paper_19326203_v8_n1_p_Alvarez
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