Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells

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We assessed the intracellular association states of the Parkinson's disease related protein α-synuclein (AS) in living cells by transfection with a functional recombinant mutant protein (AS-C4) bearing a tetracysteine tag binding the fluorogenic biarsenical ligands FlAsH and ReAsH, The aggregat...

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Autores principales:	Roberti, M.J., Jovin, T.M., Jares-Erijman, E.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2011
Materias:	alpha synuclein amyloid cysteine FIAsH ligand ligand membrane protein monomer REAsH ligand recombinant mutant protein recombinant protein tetracysteine unclassified drug fluorescent dye hybrid protein anisotropy article brightness chemical structure confocal fluorescence anisotropy controlled study diffusion coefficient fluorescence microscopy fluorescence recovery after photobleaching genetic transfection human human cell human tissue in situ hybridization molecular density molecular dynamics Parkinson disease protein aggregation protein structure quantitative analysis translation regulation algorithm chemistry confocal microscopy fluorescence fluorescence polarization genetics kinetics metabolism methodology mutation single cell analysis tumor cell line Algorithms alpha-Synuclein Amyloid Cell Line, Tumor Cysteine Fluorescence Fluorescence Polarization Fluorescence Recovery After Photobleaching Fluorescent Dyes Humans Kinetics Microscopy, Confocal Mutation Recombinant Fusion Proteins Single-Cell Analysis Transfection
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v6_n8_p_Roberti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_19326203_v6_n8_p_Roberti
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spelling	paperaa:paper_19326203_v6_n8_p_Roberti2023-06-12T16:51:31Z Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells PLoS ONE 2011;6(8) Roberti, M.J. Jovin, T.M. Jares-Erijman, E. alpha synuclein amyloid cysteine FIAsH ligand ligand membrane protein monomer REAsH ligand recombinant mutant protein recombinant protein tetracysteine unclassified drug alpha synuclein amyloid cysteine fluorescent dye hybrid protein anisotropy article brightness chemical structure confocal fluorescence anisotropy controlled study diffusion coefficient fluorescence microscopy fluorescence recovery after photobleaching genetic transfection human human cell human tissue in situ hybridization molecular density molecular dynamics Parkinson disease protein aggregation protein structure quantitative analysis translation regulation algorithm chemistry confocal microscopy fluorescence fluorescence polarization genetics kinetics metabolism methodology mutation single cell analysis tumor cell line Algorithms alpha-Synuclein Amyloid Cell Line, Tumor Cysteine Fluorescence Fluorescence Polarization Fluorescence Recovery After Photobleaching Fluorescent Dyes Humans Kinetics Microscopy, Confocal Mutation Recombinant Fusion Proteins Single-Cell Analysis Transfection We assessed the intracellular association states of the Parkinson's disease related protein α-synuclein (AS) in living cells by transfection with a functional recombinant mutant protein (AS-C4) bearing a tetracysteine tag binding the fluorogenic biarsenical ligands FlAsH and ReAsH, The aggregation states of AS-C4 were assessed by in situ microscopy of molecular translational mobility with FRAP (fluorescence recovery after photobleaching) and of local molecular density with confocal fluorescence anisotropy (CFA). FRAP recovery was quantitative and rapid in regions of free protein, whereas AS in larger aggregates was>80% immobile. A small 16% recovery characterized by an apparent diffusion constant of 0.03-0.04 μm 2/s was attributed to the dynamics of smaller, associated forms of AS-C4 and the exchange of mobile species with the larger immobile aggregates. By CFA, the larger aggregates exhibited high brightness and very low anisotropy, consistent with homoFRET between closely packed AS, for which a Förster distance (R o) of 5.3 nm was calculated. Other bright regions had high anisotropy values, close to that of monomeric AS, and indicative of membrane-associated protein with both low mobility and low degree of association. The anisotropy-fluorescence intensity correlations also revealed regions of free protein or of small aggregates, undetectable by conventional fluorescence imaging alone. The combined strategy (FRAP+CFA) provides a highly sensitive means for elucidating both the dynamics and structural features of protein aggregates and other intracellular complexes in living cells, and can be extended to other amyloid systems and to drug screening protocols. © 2011 Roberti et al. Fil:Roberti, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v6_n8_p_Roberti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	alpha synuclein amyloid cysteine FIAsH ligand ligand membrane protein monomer REAsH ligand recombinant mutant protein recombinant protein tetracysteine unclassified drug alpha synuclein amyloid cysteine fluorescent dye hybrid protein anisotropy article brightness chemical structure confocal fluorescence anisotropy controlled study diffusion coefficient fluorescence microscopy fluorescence recovery after photobleaching genetic transfection human human cell human tissue in situ hybridization molecular density molecular dynamics Parkinson disease protein aggregation protein structure quantitative analysis translation regulation algorithm chemistry confocal microscopy fluorescence fluorescence polarization genetics kinetics metabolism methodology mutation single cell analysis tumor cell line Algorithms alpha-Synuclein Amyloid Cell Line, Tumor Cysteine Fluorescence Fluorescence Polarization Fluorescence Recovery After Photobleaching Fluorescent Dyes Humans Kinetics Microscopy, Confocal Mutation Recombinant Fusion Proteins Single-Cell Analysis Transfection
spellingShingle	alpha synuclein amyloid cysteine FIAsH ligand ligand membrane protein monomer REAsH ligand recombinant mutant protein recombinant protein tetracysteine unclassified drug alpha synuclein amyloid cysteine fluorescent dye hybrid protein anisotropy article brightness chemical structure confocal fluorescence anisotropy controlled study diffusion coefficient fluorescence microscopy fluorescence recovery after photobleaching genetic transfection human human cell human tissue in situ hybridization molecular density molecular dynamics Parkinson disease protein aggregation protein structure quantitative analysis translation regulation algorithm chemistry confocal microscopy fluorescence fluorescence polarization genetics kinetics metabolism methodology mutation single cell analysis tumor cell line Algorithms alpha-Synuclein Amyloid Cell Line, Tumor Cysteine Fluorescence Fluorescence Polarization Fluorescence Recovery After Photobleaching Fluorescent Dyes Humans Kinetics Microscopy, Confocal Mutation Recombinant Fusion Proteins Single-Cell Analysis Transfection Roberti, M.J. Jovin, T.M. Jares-Erijman, E. Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells
topic_facet	alpha synuclein amyloid cysteine FIAsH ligand ligand membrane protein monomer REAsH ligand recombinant mutant protein recombinant protein tetracysteine unclassified drug alpha synuclein amyloid cysteine fluorescent dye hybrid protein anisotropy article brightness chemical structure confocal fluorescence anisotropy controlled study diffusion coefficient fluorescence microscopy fluorescence recovery after photobleaching genetic transfection human human cell human tissue in situ hybridization molecular density molecular dynamics Parkinson disease protein aggregation protein structure quantitative analysis translation regulation algorithm chemistry confocal microscopy fluorescence fluorescence polarization genetics kinetics metabolism methodology mutation single cell analysis tumor cell line Algorithms alpha-Synuclein Amyloid Cell Line, Tumor Cysteine Fluorescence Fluorescence Polarization Fluorescence Recovery After Photobleaching Fluorescent Dyes Humans Kinetics Microscopy, Confocal Mutation Recombinant Fusion Proteins Single-Cell Analysis Transfection
description	We assessed the intracellular association states of the Parkinson's disease related protein α-synuclein (AS) in living cells by transfection with a functional recombinant mutant protein (AS-C4) bearing a tetracysteine tag binding the fluorogenic biarsenical ligands FlAsH and ReAsH, The aggregation states of AS-C4 were assessed by in situ microscopy of molecular translational mobility with FRAP (fluorescence recovery after photobleaching) and of local molecular density with confocal fluorescence anisotropy (CFA). FRAP recovery was quantitative and rapid in regions of free protein, whereas AS in larger aggregates was>80% immobile. A small 16% recovery characterized by an apparent diffusion constant of 0.03-0.04 μm 2/s was attributed to the dynamics of smaller, associated forms of AS-C4 and the exchange of mobile species with the larger immobile aggregates. By CFA, the larger aggregates exhibited high brightness and very low anisotropy, consistent with homoFRET between closely packed AS, for which a Förster distance (R o) of 5.3 nm was calculated. Other bright regions had high anisotropy values, close to that of monomeric AS, and indicative of membrane-associated protein with both low mobility and low degree of association. The anisotropy-fluorescence intensity correlations also revealed regions of free protein or of small aggregates, undetectable by conventional fluorescence imaging alone. The combined strategy (FRAP+CFA) provides a highly sensitive means for elucidating both the dynamics and structural features of protein aggregates and other intracellular complexes in living cells, and can be extended to other amyloid systems and to drug screening protocols. © 2011 Roberti et al.
format	Artículo Artículo publishedVersion
author	Roberti, M.J. Jovin, T.M. Jares-Erijman, E.
author_facet	Roberti, M.J. Jovin, T.M. Jares-Erijman, E.
author_sort	Roberti, M.J.
title	Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells
title_short	Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells
title_full	Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells
title_fullStr	Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells
title_full_unstemmed	Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells
title_sort	confocal fluorescence anisotropy and frap imaging of α-synuclein amyloid aggregates in living cells
publishDate	2011
url	http://hdl.handle.net/20.500.12110/paper_19326203_v6_n8_p_Roberti
work_keys_str_mv	AT robertimj confocalfluorescenceanisotropyandfrapimagingofasynucleinamyloidaggregatesinlivingcells AT jovintm confocalfluorescenceanisotropyandfrapimagingofasynucleinamyloidaggregatesinlivingcells AT jareserijmane confocalfluorescenceanisotropyandfrapimagingofasynucleinamyloidaggregatesinlivingcells
_version_	1769810146410627072

Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells

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