The role of pH fronts in reversible electroporation

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We present experimental measurements and theoretical predictions of ion transport in agar gels during reversible electroporation (ECT) for conditions typical to many clinical studies found in the literature, revealing the presence of pH fronts emerging from both electrodes. These results suggest tha...

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Autores principales: Turjanski, P., Olaiz, N., Maglietti, F., Michinski, S., Suárez, C., Molina, F.V., Marshall, G.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2011
Materias:
acidification
article
comparative study
controlled study
electrode
electrogenetherapy
electroporation
electrostimulation therapy
gene therapy
ion transport
pH
reversible electroporation
tissue necrosis
Animals
Electrochemistry
Electrodes
Electroporation
Genetic Techniques
Genetic Therapy
Humans
Hydrogen-Ion Concentration
Models, Theoretical
Necrosis
Plasmids
Time Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Turjanski
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_19326203_v6_n4_p_Turjanski
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spelling paperaa:paper_19326203_v6_n4_p_Turjanski2023-06-12T16:51:29Z The role of pH fronts in reversible electroporation PLoS ONE 2011;6(4) Turjanski, P. Olaiz, N. Maglietti, F. Michinski, S. Suárez, C. Molina, F.V. Marshall, G. acidification article comparative study controlled study electrode electrogenetherapy electroporation electrostimulation therapy gene therapy ion transport pH reversible electroporation tissue necrosis Animals Electrochemistry Electrodes Electroporation Genetic Techniques Genetic Therapy Humans Hydrogen-Ion Concentration Models, Theoretical Necrosis Plasmids Time Factors We present experimental measurements and theoretical predictions of ion transport in agar gels during reversible electroporation (ECT) for conditions typical to many clinical studies found in the literature, revealing the presence of pH fronts emerging from both electrodes. These results suggest that pH fronts are immediate and substantial. Since they might give rise to tissue necrosis, an unwanted condition in clinical applications of ECT as well as in irreversible electroporation (IRE) and in electrogenetherapy (EGT), it is important to quantify their extent and evolution. Here, a tracking technique is used to follow the space-time evolution of these pH fronts. It is found that they scale in time as t1/2 characteristic of a predominantly diffusive process. Comparing ECT pH fronts with those arising in electrotherapy (EChT), another treatment applying constant electric fields whose main goal is tissue necrosis, a striking result is observed: anodic acidification is larger in ECT than in EChT, suggesting that tissue necrosis could also be greater. Ways to minimize these adverse effects in ECT are suggested. © 2011 Turjanski et al. Fil:Turjanski, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Suárez, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molina, F.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Turjanski
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic acidification
article
comparative study
controlled study
electrode
electrogenetherapy
electroporation
electrostimulation therapy
gene therapy
ion transport
pH
reversible electroporation
tissue necrosis
Animals
Electrochemistry
Electrodes
Electroporation
Genetic Techniques
Genetic Therapy
Humans
Hydrogen-Ion Concentration
Models, Theoretical
Necrosis
Plasmids
Time Factors
spellingShingle acidification
article
comparative study
controlled study
electrode
electrogenetherapy
electroporation
electrostimulation therapy
gene therapy
ion transport
pH
reversible electroporation
tissue necrosis
Animals
Electrochemistry
Electrodes
Electroporation
Genetic Techniques
Genetic Therapy
Humans
Hydrogen-Ion Concentration
Models, Theoretical
Necrosis
Plasmids
Time Factors
Turjanski, P.
Olaiz, N.
Maglietti, F.
Michinski, S.
Suárez, C.
Molina, F.V.
Marshall, G.
The role of pH fronts in reversible electroporation
topic_facet acidification
article
comparative study
controlled study
electrode
electrogenetherapy
electroporation
electrostimulation therapy
gene therapy
ion transport
pH
reversible electroporation
tissue necrosis
Animals
Electrochemistry
Electrodes
Electroporation
Genetic Techniques
Genetic Therapy
Humans
Hydrogen-Ion Concentration
Models, Theoretical
Necrosis
Plasmids
Time Factors
description We present experimental measurements and theoretical predictions of ion transport in agar gels during reversible electroporation (ECT) for conditions typical to many clinical studies found in the literature, revealing the presence of pH fronts emerging from both electrodes. These results suggest that pH fronts are immediate and substantial. Since they might give rise to tissue necrosis, an unwanted condition in clinical applications of ECT as well as in irreversible electroporation (IRE) and in electrogenetherapy (EGT), it is important to quantify their extent and evolution. Here, a tracking technique is used to follow the space-time evolution of these pH fronts. It is found that they scale in time as t1/2 characteristic of a predominantly diffusive process. Comparing ECT pH fronts with those arising in electrotherapy (EChT), another treatment applying constant electric fields whose main goal is tissue necrosis, a striking result is observed: anodic acidification is larger in ECT than in EChT, suggesting that tissue necrosis could also be greater. Ways to minimize these adverse effects in ECT are suggested. © 2011 Turjanski et al.
format Artículo
Artículo
publishedVersion
author Turjanski, P.
Olaiz, N.
Maglietti, F.
Michinski, S.
Suárez, C.
Molina, F.V.
Marshall, G.
author_facet Turjanski, P.
Olaiz, N.
Maglietti, F.
Michinski, S.
Suárez, C.
Molina, F.V.
Marshall, G.
author_sort Turjanski, P.
title The role of pH fronts in reversible electroporation
title_short The role of pH fronts in reversible electroporation
title_full The role of pH fronts in reversible electroporation
title_fullStr The role of pH fronts in reversible electroporation
title_full_unstemmed The role of pH fronts in reversible electroporation
title_sort role of ph fronts in reversible electroporation
publishDate 2011
url http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Turjanski
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