Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed

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Sulfated polysaccharides from Laminaria saccharina (new name: Saccharina latissima) brown seaweed show promising activity for the treatment of inflammation, thrombosis, and cancer; yet the molecular mechanisms underlying these properties remain poorly understood. The aim of this work was to characte...

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Autores principales:	Croci, D.O., Cumashi, A., Ushakova, N.A., Preobrazhenskaya, M.E., Piccoli, A., Totani, L., Ustyuzhanina, N.E., Bilan, M.I., Usov, A.I., Grachev, A.A., Morozevich, G.E., Berman, A.E., Sanderson, C.J., Kelly, M., Gregorio, P., Rossi, C., Tinari, N., Iacobelli, S., Rabinovich, G.A., Nifantiev, N.E.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2011
Materias:	bovine serum albumin carbohydrate derivative fibroblast growth factor hemoglobin o sulfated mannoglucuronofucan derivative plasminogen activator inhibitor 1 polysaccharide sulfate sulfated fucan derivative unclassified drug angiogenesis inhibitor anticoagulant agent antiinflammatory agent biological product fucoidin fucose polysaccharide angiogenesis animal experiment animal model antiangiogenic activity anticoagulation antiinflammatory activity antineoplastic activity article biological activity breast cancer cancer growth cancer inhibition cell level cell surface controlled study drug determination drug efficacy drug isolation drug structure endothelium cell fibroblast human human cell in vitro study in vivo study Laminaria Laminaria saccharina leukocyte migration inhibition melanoma melanoma cell mouse nonhuman partial thromboplastin time peritonitis rat thrombocyte adhesion tumor vascularization animal brown alga C57BL mouse cell culture chemistry drug effect evaluation female inflammation metabolism pathology physiology seaweed Wistar rat Bovinae Mus Rattus Saccharina latissima Angiogenesis Inhibitors Animals Anti-Inflammatory Agents Anticoagulants Biological Products Cells, Cultured Endothelial Cells Female Fucose Humans Inflammation Mice Mice, Inbred C57BL Neovascularization, Physiologic Phaeophyta Polysaccharides Rats Rats, Wistar Seaweed
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v6_n2_p_Croci
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_19326203_v6_n2_p_Croci
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	bovine serum albumin carbohydrate derivative fibroblast growth factor hemoglobin o sulfated mannoglucuronofucan derivative plasminogen activator inhibitor 1 polysaccharide sulfate sulfated fucan derivative unclassified drug angiogenesis inhibitor anticoagulant agent antiinflammatory agent biological product fucoidin fucose polysaccharide angiogenesis animal experiment animal model antiangiogenic activity anticoagulation antiinflammatory activity antineoplastic activity article biological activity breast cancer cancer growth cancer inhibition cell level cell surface controlled study drug determination drug efficacy drug isolation drug structure endothelium cell fibroblast human human cell in vitro study in vivo study Laminaria Laminaria saccharina leukocyte migration inhibition melanoma melanoma cell mouse nonhuman partial thromboplastin time peritonitis rat thrombocyte adhesion tumor vascularization animal brown alga C57BL mouse cell culture chemistry drug effect evaluation female inflammation Laminaria metabolism pathology physiology seaweed Wistar rat Bovinae Mus Rattus Saccharina latissima Angiogenesis Inhibitors Animals Anti-Inflammatory Agents Anticoagulants Biological Products Cells, Cultured Endothelial Cells Female Fucose Humans Inflammation Laminaria Mice Mice, Inbred C57BL Neovascularization, Physiologic Phaeophyta Polysaccharides Rats Rats, Wistar Seaweed
spellingShingle	bovine serum albumin carbohydrate derivative fibroblast growth factor hemoglobin o sulfated mannoglucuronofucan derivative plasminogen activator inhibitor 1 polysaccharide sulfate sulfated fucan derivative unclassified drug angiogenesis inhibitor anticoagulant agent antiinflammatory agent biological product fucoidin fucose polysaccharide angiogenesis animal experiment animal model antiangiogenic activity anticoagulation antiinflammatory activity antineoplastic activity article biological activity breast cancer cancer growth cancer inhibition cell level cell surface controlled study drug determination drug efficacy drug isolation drug structure endothelium cell fibroblast human human cell in vitro study in vivo study Laminaria Laminaria saccharina leukocyte migration inhibition melanoma melanoma cell mouse nonhuman partial thromboplastin time peritonitis rat thrombocyte adhesion tumor vascularization animal brown alga C57BL mouse cell culture chemistry drug effect evaluation female inflammation Laminaria metabolism pathology physiology seaweed Wistar rat Bovinae Mus Rattus Saccharina latissima Angiogenesis Inhibitors Animals Anti-Inflammatory Agents Anticoagulants Biological Products Cells, Cultured Endothelial Cells Female Fucose Humans Inflammation Laminaria Mice Mice, Inbred C57BL Neovascularization, Physiologic Phaeophyta Polysaccharides Rats Rats, Wistar Seaweed Croci, D.O. Cumashi, A. Ushakova, N.A. Preobrazhenskaya, M.E. Piccoli, A. Totani, L. Ustyuzhanina, N.E. Bilan, M.I. Usov, A.I. Grachev, A.A. Morozevich, G.E. Berman, A.E. Sanderson, C.J. Kelly, M. Gregorio, P. Rossi, C. Tinari, N. Iacobelli, S. Rabinovich, G.A. Nifantiev, N.E. Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
topic_facet	bovine serum albumin carbohydrate derivative fibroblast growth factor hemoglobin o sulfated mannoglucuronofucan derivative plasminogen activator inhibitor 1 polysaccharide sulfate sulfated fucan derivative unclassified drug angiogenesis inhibitor anticoagulant agent antiinflammatory agent biological product fucoidin fucose polysaccharide angiogenesis animal experiment animal model antiangiogenic activity anticoagulation antiinflammatory activity antineoplastic activity article biological activity breast cancer cancer growth cancer inhibition cell level cell surface controlled study drug determination drug efficacy drug isolation drug structure endothelium cell fibroblast human human cell in vitro study in vivo study Laminaria Laminaria saccharina leukocyte migration inhibition melanoma melanoma cell mouse nonhuman partial thromboplastin time peritonitis rat thrombocyte adhesion tumor vascularization animal brown alga C57BL mouse cell culture chemistry drug effect evaluation female inflammation Laminaria metabolism pathology physiology seaweed Wistar rat Bovinae Mus Rattus Saccharina latissima Angiogenesis Inhibitors Animals Anti-Inflammatory Agents Anticoagulants Biological Products Cells, Cultured Endothelial Cells Female Fucose Humans Inflammation Laminaria Mice Mice, Inbred C57BL Neovascularization, Physiologic Phaeophyta Polysaccharides Rats Rats, Wistar Seaweed
description	Sulfated polysaccharides from Laminaria saccharina (new name: Saccharina latissima) brown seaweed show promising activity for the treatment of inflammation, thrombosis, and cancer; yet the molecular mechanisms underlying these properties remain poorly understood. The aim of this work was to characterize, using in vitro and in vivo strategies, the anti-inflammatory, anti-coagulant, anti-angiogenic, and anti-tumor activities of two main sulfated polysaccharide fractions obtained from L. saccharina: a) L.s.-1.0 fraction mainly consisting of O-sulfated mannoglucuronofucans and b) L.s.-1.25 fraction mainly composed of sulfated fucans. Both fractions inhibited leukocyte recruitment in a model of inflammation in rats, although L.s.-1.25 appeared to be more active than L.s.-1.0. Also, these fractions inhibited neutrophil adhesion to platelets under flow. Only fraction L.s.-1.25, but not L.s.-1.0, displayed anticoagulant activity as measured by the activated partial thromboplastin time. Investigation of these fractions in angiogenesis settings revealed that only L.s.-1.25 strongly inhibited fetal bovine serum (FBS) induced in vitro tubulogenesis. This effect correlated with a reduction in plasminogen activator inhibitor-1 (PAI-1) levels in L.s.-1.25-treated endothelial cells. Furthermore, only parent sulfated polysaccharides from L. saccharina (L.s.-P) and its fraction L.s.-1.25 were powerful inhibitors of basic fibroblast growth factor (bFGF) induced pathways. Consistently, the L.s.-1.25 fraction as well as L.s.-P successfully interfered with fibroblast binding to human bFGF. The incorporation of L.s.-P or L.s.-1.25, but not L.s.-1.0 into Matrigel plugs containing melanoma cells induced a significant reduction in hemoglobin content as well in the frequency of tumor-associated blood vessels. Moreover, i.p. administrations of L.s.-1.25, as well as L.s.-P, but not L.s.-1.0, resulted in a significant reduction of tumor growth when inoculated into syngeneic mice. Finally, L.s.-1.25 markedly inhibited breast cancer cell adhesion to human platelet-coated surfaces. Thus, sulfated fucans are mainly responsible for the anti-inflammatory, anticoagulant, antiangiogenic, and antitumor activities of sulfated polysaccharides from L. saccharina brown seaweed. © 2011 Croci et al.
format	Artículo Artículo publishedVersion
author	Croci, D.O. Cumashi, A. Ushakova, N.A. Preobrazhenskaya, M.E. Piccoli, A. Totani, L. Ustyuzhanina, N.E. Bilan, M.I. Usov, A.I. Grachev, A.A. Morozevich, G.E. Berman, A.E. Sanderson, C.J. Kelly, M. Gregorio, P. Rossi, C. Tinari, N. Iacobelli, S. Rabinovich, G.A. Nifantiev, N.E.
author_facet	Croci, D.O. Cumashi, A. Ushakova, N.A. Preobrazhenskaya, M.E. Piccoli, A. Totani, L. Ustyuzhanina, N.E. Bilan, M.I. Usov, A.I. Grachev, A.A. Morozevich, G.E. Berman, A.E. Sanderson, C.J. Kelly, M. Gregorio, P. Rossi, C. Tinari, N. Iacobelli, S. Rabinovich, G.A. Nifantiev, N.E.
author_sort	Croci, D.O.
title	Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
title_short	Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
title_full	Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
title_fullStr	Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
title_full_unstemmed	Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
title_sort	fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
publishDate	2011
url	http://hdl.handle.net/20.500.12110/paper_19326203_v6_n2_p_Croci
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spelling	paperaa:paper_19326203_v6_n2_p_Croci2023-06-12T16:51:28Z Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed PLoS ONE 2011;6(2) Croci, D.O. Cumashi, A. Ushakova, N.A. Preobrazhenskaya, M.E. Piccoli, A. Totani, L. Ustyuzhanina, N.E. Bilan, M.I. Usov, A.I. Grachev, A.A. Morozevich, G.E. Berman, A.E. Sanderson, C.J. Kelly, M. Gregorio, P. Rossi, C. Tinari, N. Iacobelli, S. Rabinovich, G.A. Nifantiev, N.E. bovine serum albumin carbohydrate derivative fibroblast growth factor hemoglobin o sulfated mannoglucuronofucan derivative plasminogen activator inhibitor 1 polysaccharide sulfate sulfated fucan derivative unclassified drug angiogenesis inhibitor anticoagulant agent antiinflammatory agent biological product fucoidin fucose polysaccharide angiogenesis animal experiment animal model antiangiogenic activity anticoagulation antiinflammatory activity antineoplastic activity article biological activity breast cancer cancer growth cancer inhibition cell level cell surface controlled study drug determination drug efficacy drug isolation drug structure endothelium cell fibroblast human human cell in vitro study in vivo study Laminaria Laminaria saccharina leukocyte migration inhibition melanoma melanoma cell mouse nonhuman partial thromboplastin time peritonitis rat thrombocyte adhesion tumor vascularization animal brown alga C57BL mouse cell culture chemistry drug effect evaluation female inflammation Laminaria metabolism pathology physiology seaweed Wistar rat Bovinae Mus Rattus Saccharina latissima Angiogenesis Inhibitors Animals Anti-Inflammatory Agents Anticoagulants Biological Products Cells, Cultured Endothelial Cells Female Fucose Humans Inflammation Laminaria Mice Mice, Inbred C57BL Neovascularization, Physiologic Phaeophyta Polysaccharides Rats Rats, Wistar Seaweed Sulfated polysaccharides from Laminaria saccharina (new name: Saccharina latissima) brown seaweed show promising activity for the treatment of inflammation, thrombosis, and cancer; yet the molecular mechanisms underlying these properties remain poorly understood. The aim of this work was to characterize, using in vitro and in vivo strategies, the anti-inflammatory, anti-coagulant, anti-angiogenic, and anti-tumor activities of two main sulfated polysaccharide fractions obtained from L. saccharina: a) L.s.-1.0 fraction mainly consisting of O-sulfated mannoglucuronofucans and b) L.s.-1.25 fraction mainly composed of sulfated fucans. Both fractions inhibited leukocyte recruitment in a model of inflammation in rats, although L.s.-1.25 appeared to be more active than L.s.-1.0. Also, these fractions inhibited neutrophil adhesion to platelets under flow. Only fraction L.s.-1.25, but not L.s.-1.0, displayed anticoagulant activity as measured by the activated partial thromboplastin time. Investigation of these fractions in angiogenesis settings revealed that only L.s.-1.25 strongly inhibited fetal bovine serum (FBS) induced in vitro tubulogenesis. This effect correlated with a reduction in plasminogen activator inhibitor-1 (PAI-1) levels in L.s.-1.25-treated endothelial cells. Furthermore, only parent sulfated polysaccharides from L. saccharina (L.s.-P) and its fraction L.s.-1.25 were powerful inhibitors of basic fibroblast growth factor (bFGF) induced pathways. Consistently, the L.s.-1.25 fraction as well as L.s.-P successfully interfered with fibroblast binding to human bFGF. The incorporation of L.s.-P or L.s.-1.25, but not L.s.-1.0 into Matrigel plugs containing melanoma cells induced a significant reduction in hemoglobin content as well in the frequency of tumor-associated blood vessels. Moreover, i.p. administrations of L.s.-1.25, as well as L.s.-P, but not L.s.-1.0, resulted in a significant reduction of tumor growth when inoculated into syngeneic mice. Finally, L.s.-1.25 markedly inhibited breast cancer cell adhesion to human platelet-coated surfaces. Thus, sulfated fucans are mainly responsible for the anti-inflammatory, anticoagulant, antiangiogenic, and antitumor activities of sulfated polysaccharides from L. saccharina brown seaweed. © 2011 Croci et al. Fil:Croci, D.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v6_n2_p_Croci

Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed

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