A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells

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Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct p...

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Autores principales:	Galli, S., Jahn, O., Hitt, R., Hesse, D., Opitz, L., Plessmann, U., Urlaub, H., Poderoso, J.J., Jares-Erijman, E.A., Jovin, T.M.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2009
Materias:	histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v4_n10_p_Galli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_19326203_v4_n10_p_Galli2023-06-12T16:51:24Z A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells PLoS ONE 2009;4(10) Galli, S. Jahn, O. Hitt, R. Hesse, D. Opitz, L. Plessmann, U. Urlaub, H. Poderoso, J.J. Jares-Erijman, E.A. Jovin, T.M. histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct phosphorylation of downstream effectors located throughout the cell. In this study we determined that hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus. In the mitochondrial environment, hERK1 physically associates with (i) at least 5 mitochondrial proteins with functions related to transport (i.e. VDAC1), signalling, and metabolism; (ii) histones H2A and H4; and (iii) other cytosolic proteins. This work indicates for the first time the presence of diverse ERK-complexes in mitochondria and thus provides a new perspective for assessing the functions of ERK1 in the regulation of cellular signalling and trafficking in HeLa cells. © 2009 Galli et al. Fil:Galli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v4_n10_p_Galli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
spellingShingle	histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Galli, S. Jahn, O. Hitt, R. Hesse, D. Opitz, L. Plessmann, U. Urlaub, H. Poderoso, J.J. Jares-Erijman, E.A. Jovin, T.M. A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells
topic_facet	histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
description	Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct phosphorylation of downstream effectors located throughout the cell. In this study we determined that hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus. In the mitochondrial environment, hERK1 physically associates with (i) at least 5 mitochondrial proteins with functions related to transport (i.e. VDAC1), signalling, and metabolism; (ii) histones H2A and H4; and (iii) other cytosolic proteins. This work indicates for the first time the presence of diverse ERK-complexes in mitochondria and thus provides a new perspective for assessing the functions of ERK1 in the regulation of cellular signalling and trafficking in HeLa cells. © 2009 Galli et al.
format	Artículo Artículo publishedVersion
author	Galli, S. Jahn, O. Hitt, R. Hesse, D. Opitz, L. Plessmann, U. Urlaub, H. Poderoso, J.J. Jares-Erijman, E.A. Jovin, T.M.
author_facet	Galli, S. Jahn, O. Hitt, R. Hesse, D. Opitz, L. Plessmann, U. Urlaub, H. Poderoso, J.J. Jares-Erijman, E.A. Jovin, T.M.
author_sort	Galli, S.
title	A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells
title_short	A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells
title_full	A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells
title_fullStr	A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells
title_full_unstemmed	A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells
title_sort	new paradigm for mapk: structural interactions of herk1 with mitochondria in hela cells
publishDate	2009
url	http://hdl.handle.net/20.500.12110/paper_19326203_v4_n10_p_Galli
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A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells

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