Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species

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By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method...

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Detalles Bibliográficos
Autores principales: Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., Eggeling, C., Schönle, A., Hell, S.W.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2008
Materias:
Imaging techniques
Mammals
Molecular structure
Optical switches
Biological specimens
Color channels
Far fields
Functional imaging
Mammalian cells
Molecular species
Multicolor imaging
New class
Photoswitching
Single-molecule levels
Spatial resolutions
Switchable
Fluorescence
fluorescent dye
animal
article
cell line
chemical structure
chemistry
color
methodology
microscopy
Animals
Cell Line
Color
Fluorescent Dyes
Microscopy
Molecular Structure
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15306984_v8_n8_p2463_Bossi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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