Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species

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By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method...

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Autores principales: Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., Eggeling, C., Schönle, A., Hell, S.W.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2008
Materias:
Imaging techniques
Mammals
Molecular structure
Optical switches
Biological specimens
Color channels
Far fields
Functional imaging
Mammalian cells
Molecular species
Multicolor imaging
New class
Photoswitching
Single-molecule levels
Spatial resolutions
Switchable
Fluorescence
fluorescent dye
animal
article
cell line
chemical structure
chemistry
color
methodology
microscopy
Animals
Cell Line
Color
Fluorescent Dyes
Microscopy
Molecular Structure
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15306984_v8_n8_p2463_Bossi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paperaa:paper_15306984_v8_n8_p2463_Bossi2023-06-12T16:50:37Z Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species Nano Lett. 2008;8(8):2463-2468 Bossi, M. Föiling, J. Belov, V.N. Boyarskiy, V.P. Medda, R. Egner, A. Eggeling, C. Schönle, A. Hell, S.W. Imaging techniques Mammals Molecular structure Optical switches Biological specimens Color channels Far fields Functional imaging Mammalian cells Molecular species Multicolor imaging New class Photoswitching Single-molecule levels Spatial resolutions Switchable Fluorescence fluorescent dye animal article cell line chemical structure chemistry color methodology microscopy Animals Cell Line Color Fluorescent Dyes Microscopy Molecular Structure By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. © 2008 American Chemical Society. Fil:Bossi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15306984_v8_n8_p2463_Bossi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Imaging techniques
Mammals
Molecular structure
Optical switches
Biological specimens
Color channels
Far fields
Functional imaging
Mammalian cells
Molecular species
Multicolor imaging
New class
Photoswitching
Single-molecule levels
Spatial resolutions
Switchable
Fluorescence
fluorescent dye
animal
article
cell line
chemical structure
chemistry
color
methodology
microscopy
Animals
Cell Line
Color
Fluorescent Dyes
Microscopy
Molecular Structure
spellingShingle Imaging techniques
Mammals
Molecular structure
Optical switches
Biological specimens
Color channels
Far fields
Functional imaging
Mammalian cells
Molecular species
Multicolor imaging
New class
Photoswitching
Single-molecule levels
Spatial resolutions
Switchable
Fluorescence
fluorescent dye
animal
article
cell line
chemical structure
chemistry
color
methodology
microscopy
Animals
Cell Line
Color
Fluorescent Dyes
Microscopy
Molecular Structure
Bossi, M.
Föiling, J.
Belov, V.N.
Boyarskiy, V.P.
Medda, R.
Egner, A.
Eggeling, C.
Schönle, A.
Hell, S.W.
Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
topic_facet Imaging techniques
Mammals
Molecular structure
Optical switches
Biological specimens
Color channels
Far fields
Functional imaging
Mammalian cells
Molecular species
Multicolor imaging
New class
Photoswitching
Single-molecule levels
Spatial resolutions
Switchable
Fluorescence
fluorescent dye
animal
article
cell line
chemical structure
chemistry
color
methodology
microscopy
Animals
Cell Line
Color
Fluorescent Dyes
Microscopy
Molecular Structure
description By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. © 2008 American Chemical Society.
format Artículo
Artículo
publishedVersion
author Bossi, M.
Föiling, J.
Belov, V.N.
Boyarskiy, V.P.
Medda, R.
Egner, A.
Eggeling, C.
Schönle, A.
Hell, S.W.
author_facet Bossi, M.
Föiling, J.
Belov, V.N.
Boyarskiy, V.P.
Medda, R.
Egner, A.
Eggeling, C.
Schönle, A.
Hell, S.W.
author_sort Bossi, M.
title Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
title_short Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
title_full Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
title_fullStr Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
title_full_unstemmed Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
title_sort multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
publishDate 2008
url http://hdl.handle.net/20.500.12110/paper_15306984_v8_n8_p2463_Bossi
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