Inhibitory effect of leptin on the rat ovary during the ovulatory process

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The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation...

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Autores principales:	Ricci, A.G., Di Yorio, M.P., Faletti, A.G.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2006
Materias:	chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_Ricci
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_14701626_v132_n5_p771_Ricci
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spelling	paperaa:paper_14701626_v132_n5_p771_Ricci2023-06-12T16:50:22Z Inhibitory effect of leptin on the rat ovary during the ovulatory process Reproduction 2006;132(5):771-780 Ricci, A.G. Di Yorio, M.P. Faletti, A.G. chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility. Fil:Ricci, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_Ricci
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae
spellingShingle	chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae Ricci, A.G. Di Yorio, M.P. Faletti, A.G. Inhibitory effect of leptin on the rat ovary during the ovulatory process
topic_facet	chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae
description	The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility.
format	Artículo Artículo publishedVersion
author	Ricci, A.G. Di Yorio, M.P. Faletti, A.G.
author_facet	Ricci, A.G. Di Yorio, M.P. Faletti, A.G.
author_sort	Ricci, A.G.
title	Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_short	Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_full	Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_fullStr	Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_full_unstemmed	Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_sort	inhibitory effect of leptin on the rat ovary during the ovulatory process
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_Ricci
work_keys_str_mv	AT ricciag inhibitoryeffectofleptinontheratovaryduringtheovulatoryprocess AT diyoriomp inhibitoryeffectofleptinontheratovaryduringtheovulatoryprocess AT falettiag inhibitoryeffectofleptinontheratovaryduringtheovulatoryprocess
_version_	1769810297785155584

Inhibitory effect of leptin on the rat ovary during the ovulatory process

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