Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels

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A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step pr...

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Autores principales:	Ferro, Y., Perullini, M., Jobbagy, M., Bilmes, S.A., Durrieu, C.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2012
Materias:	Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Biosensing Techniques Diuron Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_14248220_v12_n12_p16879_Ferro
record_format	dspace
spelling	paperaa:paper_14248220_v12_n12_p16879_Ferro2023-06-12T16:50:13Z Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels Sensors 2012;12(12):16879-16891 Ferro, Y. Perullini, M. Jobbagy, M. Bilmes, S.A. Durrieu, C. Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jobbagy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilmes, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical
spellingShingle	Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical Ferro, Y. Perullini, M. Jobbagy, M. Bilmes, S.A. Durrieu, C. Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
topic_facet	Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical
description	A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
format	Artículo Artículo publishedVersion
author	Ferro, Y. Perullini, M. Jobbagy, M. Bilmes, S.A. Durrieu, C.
author_facet	Ferro, Y. Perullini, M. Jobbagy, M. Bilmes, S.A. Durrieu, C.
author_sort	Ferro, Y.
title	Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_short	Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_full	Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_fullStr	Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_full_unstemmed	Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_sort	development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
publishDate	2012
url	http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro
work_keys_str_mv	AT ferroy developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels AT perullinim developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels AT jobbagym developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels AT bilmessa developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels AT durrieuc developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels
_version_	1769810244059267072

Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels

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