MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs

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MAGE-A genes are a subfamily of the melanoma antigen genes (MAGEs), whose expression is restricted to tumor cells of different origin and normal tissues of the human germline. Although the specific function of individual MAGE-A proteins is being currently explored, compelling evidence suggest their...

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Autores principales:	Peche, L.Y., Scolz, M., Ladelfa, M.F., Monte, M., Schneider, C.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2012
Materias:	Acetylation MAGE P53 PML Senescence Sumoylation melanoma antigen 2 PMLIV protein promyelocytic leukemia protein protein p53 Ras protein tumor suppressor protein unclassified drug apoptosis article cell aging cell count cell nucleus inclusion body cell proliferation controlled study fibroblast gene expression gene targeting histone acetylation human human cell leukemia cell oncogene priority journal promyelocytic leukemia promyelocytic leukemia nuclear bodies protein analysis protein function protein induction protein localization protein processing protein protein interaction senescence sumoylation transcription initiation tumor suppressor gene Apoptosis Cell Aging Cell Line, Tumor Fibroblasts HEK293 Cells Histone Deacetylases Humans Intranuclear Inclusion Bodies Melanoma-Specific Antigens Neoplasm Proteins Nuclear Proteins Protein Interaction Mapping ras Proteins RNA Interference RNA, Small Interfering Transcription Factors Tumor Suppressor Protein p53 Tumor Suppressor Proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13509047_v19_n6_p926_Peche
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_13509047_v19_n6_p926_Peche2023-06-12T16:49:51Z MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs Cell Death Differ. 2012;19(6):926-936 Peche, L.Y. Scolz, M. Ladelfa, M.F. Monte, M. Schneider, C. Acetylation MAGE P53 PML Senescence Sumoylation melanoma antigen 2 PMLIV protein promyelocytic leukemia protein protein p53 Ras protein tumor suppressor protein unclassified drug apoptosis article cell aging cell count cell nucleus inclusion body cell proliferation controlled study fibroblast gene expression gene targeting histone acetylation human human cell leukemia cell oncogene priority journal promyelocytic leukemia promyelocytic leukemia nuclear bodies protein analysis protein function protein induction protein localization protein processing protein protein interaction senescence sumoylation transcription initiation tumor suppressor gene Acetylation Apoptosis Cell Aging Cell Line, Tumor Fibroblasts HEK293 Cells Histone Deacetylases Humans Intranuclear Inclusion Bodies Melanoma-Specific Antigens Neoplasm Proteins Nuclear Proteins Protein Interaction Mapping ras Proteins RNA Interference RNA, Small Interfering Sumoylation Transcription Factors Tumor Suppressor Protein p53 Tumor Suppressor Proteins MAGE-A genes are a subfamily of the melanoma antigen genes (MAGEs), whose expression is restricted to tumor cells of different origin and normal tissues of the human germline. Although the specific function of individual MAGE-A proteins is being currently explored, compelling evidence suggest their involvement in the regulation of different pathways during tumor progression. We have previously reported that MageA2 binds histone deacetylase (HDAC)3 and represses p53-dependent apoptosis in response to chemotherapeutic drugs. The promyelocytic leukemia (PML) tumor suppressor is a regulator of p53 acetylation and function in cellular senescence. Here, we demonstrate that MageA2 interferes with p53 acetylation at PML-nuclear bodies (NBs) and with PMLIV-dependent activation of p53. Moreover, a fraction of MageA2 colocalizes with PML-NBs through direct association with PML, and decreases PMLIV sumoylation through an HDAC-dependent mechanism. This reduction in PML post-translational modification promotes defects in PML-NBs formation. Remarkably, we show that in human fibroblasts expressing RasV12 oncogene, MageA2 expression decreases cellular senescence and increases proliferation. These results correlate with a reduction in NBs number and an impaired p53 response. All these data suggest that MageA2, in addition to its anti-apoptotic effect, could have a novel role in the early progression to malignancy by interfering with PML/p53 function, thereby blocking the senescence program, a critical barrier against cell transformation. © 2012 Macmillan Publishers Limited. All rights reserved. Fil:Ladelfa, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Monte, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13509047_v19_n6_p926_Peche
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Acetylation MAGE P53 PML Senescence Sumoylation melanoma antigen 2 PMLIV protein promyelocytic leukemia protein protein p53 Ras protein tumor suppressor protein unclassified drug apoptosis article cell aging cell count cell nucleus inclusion body cell proliferation controlled study fibroblast gene expression gene targeting histone acetylation human human cell leukemia cell oncogene priority journal promyelocytic leukemia promyelocytic leukemia nuclear bodies protein analysis protein function protein induction protein localization protein processing protein protein interaction senescence sumoylation transcription initiation tumor suppressor gene Acetylation Apoptosis Cell Aging Cell Line, Tumor Fibroblasts HEK293 Cells Histone Deacetylases Humans Intranuclear Inclusion Bodies Melanoma-Specific Antigens Neoplasm Proteins Nuclear Proteins Protein Interaction Mapping ras Proteins RNA Interference RNA, Small Interfering Sumoylation Transcription Factors Tumor Suppressor Protein p53 Tumor Suppressor Proteins
spellingShingle	Acetylation MAGE P53 PML Senescence Sumoylation melanoma antigen 2 PMLIV protein promyelocytic leukemia protein protein p53 Ras protein tumor suppressor protein unclassified drug apoptosis article cell aging cell count cell nucleus inclusion body cell proliferation controlled study fibroblast gene expression gene targeting histone acetylation human human cell leukemia cell oncogene priority journal promyelocytic leukemia promyelocytic leukemia nuclear bodies protein analysis protein function protein induction protein localization protein processing protein protein interaction senescence sumoylation transcription initiation tumor suppressor gene Acetylation Apoptosis Cell Aging Cell Line, Tumor Fibroblasts HEK293 Cells Histone Deacetylases Humans Intranuclear Inclusion Bodies Melanoma-Specific Antigens Neoplasm Proteins Nuclear Proteins Protein Interaction Mapping ras Proteins RNA Interference RNA, Small Interfering Sumoylation Transcription Factors Tumor Suppressor Protein p53 Tumor Suppressor Proteins Peche, L.Y. Scolz, M. Ladelfa, M.F. Monte, M. Schneider, C. MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs
topic_facet	Acetylation MAGE P53 PML Senescence Sumoylation melanoma antigen 2 PMLIV protein promyelocytic leukemia protein protein p53 Ras protein tumor suppressor protein unclassified drug apoptosis article cell aging cell count cell nucleus inclusion body cell proliferation controlled study fibroblast gene expression gene targeting histone acetylation human human cell leukemia cell oncogene priority journal promyelocytic leukemia promyelocytic leukemia nuclear bodies protein analysis protein function protein induction protein localization protein processing protein protein interaction senescence sumoylation transcription initiation tumor suppressor gene Acetylation Apoptosis Cell Aging Cell Line, Tumor Fibroblasts HEK293 Cells Histone Deacetylases Humans Intranuclear Inclusion Bodies Melanoma-Specific Antigens Neoplasm Proteins Nuclear Proteins Protein Interaction Mapping ras Proteins RNA Interference RNA, Small Interfering Sumoylation Transcription Factors Tumor Suppressor Protein p53 Tumor Suppressor Proteins
description	MAGE-A genes are a subfamily of the melanoma antigen genes (MAGEs), whose expression is restricted to tumor cells of different origin and normal tissues of the human germline. Although the specific function of individual MAGE-A proteins is being currently explored, compelling evidence suggest their involvement in the regulation of different pathways during tumor progression. We have previously reported that MageA2 binds histone deacetylase (HDAC)3 and represses p53-dependent apoptosis in response to chemotherapeutic drugs. The promyelocytic leukemia (PML) tumor suppressor is a regulator of p53 acetylation and function in cellular senescence. Here, we demonstrate that MageA2 interferes with p53 acetylation at PML-nuclear bodies (NBs) and with PMLIV-dependent activation of p53. Moreover, a fraction of MageA2 colocalizes with PML-NBs through direct association with PML, and decreases PMLIV sumoylation through an HDAC-dependent mechanism. This reduction in PML post-translational modification promotes defects in PML-NBs formation. Remarkably, we show that in human fibroblasts expressing RasV12 oncogene, MageA2 expression decreases cellular senescence and increases proliferation. These results correlate with a reduction in NBs number and an impaired p53 response. All these data suggest that MageA2, in addition to its anti-apoptotic effect, could have a novel role in the early progression to malignancy by interfering with PML/p53 function, thereby blocking the senescence program, a critical barrier against cell transformation. © 2012 Macmillan Publishers Limited. All rights reserved.
format	Artículo Artículo publishedVersion
author	Peche, L.Y. Scolz, M. Ladelfa, M.F. Monte, M. Schneider, C.
author_facet	Peche, L.Y. Scolz, M. Ladelfa, M.F. Monte, M. Schneider, C.
author_sort	Peche, L.Y.
title	MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs
title_short	MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs
title_full	MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs
title_fullStr	MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs
title_full_unstemmed	MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs
title_sort	magea2 restrains cellular senescence by targeting the function of pmliv/p53 axis at the pml-nbs
publishDate	2012
url	http://hdl.handle.net/20.500.12110/paper_13509047_v19_n6_p926_Peche
work_keys_str_mv	AT pechely magea2restrainscellularsenescencebytargetingthefunctionofpmlivp53axisatthepmlnbs AT scolzm magea2restrainscellularsenescencebytargetingthefunctionofpmlivp53axisatthepmlnbs AT ladelfamf magea2restrainscellularsenescencebytargetingthefunctionofpmlivp53axisatthepmlnbs AT montem magea2restrainscellularsenescencebytargetingthefunctionofpmlivp53axisatthepmlnbs AT schneiderc magea2restrainscellularsenescencebytargetingthefunctionofpmlivp53axisatthepmlnbs
_version_	1769810041169248256

MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs

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