Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract

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Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), an...

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Autores principales:	Gholipour, Y., Nonami, H., Erra-Balsells, R.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2008
Materias:	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_10440305_v19_n12_p1841_Gholipour2023-06-12T16:48:58Z Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract J. Am. Soc. Mass Spectrom. 2008;19(12):1841-1848 Gholipour, Y. Nonami, H. Erra-Balsells, R. Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa
spellingShingle	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa Gholipour, Y. Nonami, H. Erra-Balsells, R. Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
topic_facet	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa
description	Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry.
format	Artículo Artículo publishedVersion
author	Gholipour, Y. Nonami, H. Erra-Balsells, R.
author_facet	Gholipour, Y. Nonami, H. Erra-Balsells, R.
author_sort	Gholipour, Y.
title	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_short	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_full	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_fullStr	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_full_unstemmed	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_sort	application of pressure probe and uv-maldi-tof ms for direct analysis of plant underivatized carbohydrates in subpicoliter single-cell cytoplasm extract
publishDate	2008
url	http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour
work_keys_str_mv	AT gholipoury applicationofpressureprobeanduvmalditofmsfordirectanalysisofplantunderivatizedcarbohydratesinsubpicolitersinglecellcytoplasmextract AT nonamih applicationofpressureprobeanduvmalditofmsfordirectanalysisofplantunderivatizedcarbohydratesinsubpicolitersinglecellcytoplasmextract AT errabalsellsr applicationofpressureprobeanduvmalditofmsfordirectanalysisofplantunderivatizedcarbohydratesinsubpicolitersinglecellcytoplasmextract
_version_	1769810189409583104

Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract

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