Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line

Mostrar todas las versiones(3)

Background/ Aims: Since the reversible phosphorylation of tyrosyl residues is a critical event in cellular signaling pathways activated by erythropoietin (Epo), attention has been focused on protein tyrosine phosphatases (PTPs) and their coordinated action with protein tyrosine kinases. The prototyp...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Callero, M.A., Pérez, G.M., Vittori, D.C., Pregi, N., Nesse, A.B.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2007
Materias:
Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10158987_v20_n5_p319_Callero
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_10158987_v20_n5_p319_Callero
record_format dspace
spelling paperaa:paper_10158987_v20_n5_p319_Callero2023-06-12T16:48:53Z Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line Cell. Physiol. Biochem. 2007;20(5):319-328 Callero, M.A. Pérez, G.M. Vittori, D.C. Pregi, N. Nesse, A.B. Erythropoietin Protein Tyrosine Phosphatase 1B UT-7 cell line 4 nitrophenylphosphatase erythropoietin Janus kinase 2 phosphatidylinositol 3 kinase protein tyrosine phosphatase 1B article controlled study enzyme activity enzyme phosphorylation feedback system human human cell hydrolysis priority journal protein expression protein function real time polymerase chain reaction signal transduction Western blotting 1-Phosphatidylinositol 3-Kinase Cell Line Cell Survival Down-Regulation Erythropoietin Gene Expression Regulation, Enzymologic Janus Kinase 2 Phosphorylation Protein-Tyrosine-Phosphatase Tyrphostins Background/ Aims: Since the reversible phosphorylation of tyrosyl residues is a critical event in cellular signaling pathways activated by erythropoietin (Epo), attention has been focused on protein tyrosine phosphatases (PTPs) and their coordinated action with protein tyrosine kinases. The prototypic member of the PTP family is PTP1B, a widely expressed non-receptor PTP located both in cytosol and intracellular membranes via its hydrophobic C-terminal targeting sequence. PTP1B has been implicated in the regulation of signaling pathways involving tyrosine phosphorylation induced by growth factors, cytokines, and hormones, such as the downregulation of erythropoietin and insulin receptors. However, little is known about which factor modulates the activity of this enzyme. Methods: The effect of Epo on PTP1B expression was studied in the UT-7 Epo-dependent cell line. PTP1B expression was analyzed under different conditions by Real-Time PCR and Western blot, while PTP1B phosphatase activity was determined by a p-nitrophenylphosphate hydrolysis assay. Results: Epo rapidly induced an increased expression of PTP1B which was associated with higher PTP1B tyrosine phosphorylation and phosphatase activity. The action of Epo on PTP1B induction involved Janus Kinase 2 (JAK2) and Phosphatidylinositol-3 kinase (PI3K). Conclusion: The results allow us to suggest for the first time that, besides modulating Epo/Epo receptor signaling, PTP1B undergoes feedback regulation by Epo. Copyright © 2007 S. Karger AG. Fil:Callero, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10158987_v20_n5_p319_Callero
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Erythropoietin
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
spellingShingle Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Erythropoietin
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
Callero, M.A.
Pérez, G.M.
Vittori, D.C.
Pregi, N.
Nesse, A.B.
Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
topic_facet Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Erythropoietin
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
description Background/ Aims: Since the reversible phosphorylation of tyrosyl residues is a critical event in cellular signaling pathways activated by erythropoietin (Epo), attention has been focused on protein tyrosine phosphatases (PTPs) and their coordinated action with protein tyrosine kinases. The prototypic member of the PTP family is PTP1B, a widely expressed non-receptor PTP located both in cytosol and intracellular membranes via its hydrophobic C-terminal targeting sequence. PTP1B has been implicated in the regulation of signaling pathways involving tyrosine phosphorylation induced by growth factors, cytokines, and hormones, such as the downregulation of erythropoietin and insulin receptors. However, little is known about which factor modulates the activity of this enzyme. Methods: The effect of Epo on PTP1B expression was studied in the UT-7 Epo-dependent cell line. PTP1B expression was analyzed under different conditions by Real-Time PCR and Western blot, while PTP1B phosphatase activity was determined by a p-nitrophenylphosphate hydrolysis assay. Results: Epo rapidly induced an increased expression of PTP1B which was associated with higher PTP1B tyrosine phosphorylation and phosphatase activity. The action of Epo on PTP1B induction involved Janus Kinase 2 (JAK2) and Phosphatidylinositol-3 kinase (PI3K). Conclusion: The results allow us to suggest for the first time that, besides modulating Epo/Epo receptor signaling, PTP1B undergoes feedback regulation by Epo. Copyright © 2007 S. Karger AG.
format Artículo
Artículo
publishedVersion
author Callero, M.A.
Pérez, G.M.
Vittori, D.C.
Pregi, N.
Nesse, A.B.
author_facet Callero, M.A.
Pérez, G.M.
Vittori, D.C.
Pregi, N.
Nesse, A.B.
author_sort Callero, M.A.
title Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_short Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_full Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_fullStr Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_full_unstemmed Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_sort modulation of protein tyrosine phosphatase 1b by erythropoietin in ut-7 cell line
publishDate 2007
url http://hdl.handle.net/20.500.12110/paper_10158987_v20_n5_p319_Callero
work_keys_str_mv AT calleroma modulationofproteintyrosinephosphatase1bbyerythropoietininut7cellline
AT perezgm modulationofproteintyrosinephosphatase1bbyerythropoietininut7cellline
AT vittoridc modulationofproteintyrosinephosphatase1bbyerythropoietininut7cellline
AT pregin modulationofproteintyrosinephosphatase1bbyerythropoietininut7cellline
AT nesseab modulationofproteintyrosinephosphatase1bbyerythropoietininut7cellline
_version_ 1769810396405825536

Ejemplares similares