Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochem...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2012
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino |
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paperaa:paper_01674889_v1823_n4_p900_Gambino2023-06-12T16:46:59Z Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha Biochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910 Gambino, Y.P. Pérez Pérez, A. Dueñas, J.L. Calvo, J.C. Sánchez-Margalet, V. Varone, C.L. 17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V.. Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction |
| spellingShingle |
17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction Gambino, Y.P. Pérez Pérez, A. Dueñas, J.L. Calvo, J.C. Sánchez-Margalet, V. Varone, C.L. Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
| topic_facet |
17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction |
| description |
The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V.. |
| format |
Artículo Artículo publishedVersion |
| author |
Gambino, Y.P. Pérez Pérez, A. Dueñas, J.L. Calvo, J.C. Sánchez-Margalet, V. Varone, C.L. |
| author_facet |
Gambino, Y.P. Pérez Pérez, A. Dueñas, J.L. Calvo, J.C. Sánchez-Margalet, V. Varone, C.L. |
| author_sort |
Gambino, Y.P. |
| title |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
| title_short |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
| title_full |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
| title_fullStr |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
| title_full_unstemmed |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
| title_sort |
regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
| publishDate |
2012 |
| url |
http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino |
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