Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells

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Aluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction betw...

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Autores principales:	Pérez, G., Pregi, N., Vittori, D., Di Risio, C., Garbossa, G., Nesse, A.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2005
Materias:	Aluminum Iron metabolism K562 cell line Non-transferrin bound iron transport Transferrin receptor Transferrin-mediated iron uptake aluminum antigen iron messenger RNA receptor subtype transferrin transferrin receptor article chronic myeloid leukemia controlled study flow cytometry human human cell iron depletion iron transport leukemia cell priority journal protein expression receptor binding regulatory mechanism reverse transcription polymerase chain reaction upregulation Biological Transport Butyrates Cell Differentiation Gene Expression Regulation, Neoplastic Humans Iron K562 Cells Receptors, Transferrin RNA, Messenger Transferrin
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01674889_v1745_n1_p124_Perez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_01674889_v1745_n1_p124_Perez
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spelling	paperaa:paper_01674889_v1745_n1_p124_Perez2023-06-12T16:46:58Z Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells Biochim. Biophys. Acta Mol. Cell Res. 2005;1745(1):124-130 Pérez, G. Pregi, N. Vittori, D. Di Risio, C. Garbossa, G. Nesse, A. Aluminum Iron metabolism K562 cell line Non-transferrin bound iron transport Transferrin receptor Transferrin-mediated iron uptake aluminum antigen iron messenger RNA receptor subtype transferrin transferrin receptor article chronic myeloid leukemia controlled study flow cytometry human human cell iron depletion iron transport leukemia cell priority journal protein expression receptor binding regulatory mechanism reverse transcription polymerase chain reaction upregulation Aluminum Biological Transport Butyrates Cell Differentiation Gene Expression Regulation, Neoplastic Humans Iron K562 Cells Receptors, Transferrin RNA, Messenger Transferrin Aluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction between both metals prevented normal Fe incorporation into K562 cells and triggered the upregulation of Fe transport. In the present work we demonstrated that Al modified Fe uptake without affecting the expression of Tf receptors. Both TfR and TfR2 mRNA levels, evaluated by RT-PCR, and TfR antigenic sites, analyzed by flow cytometry, were found unchanged after Al exposure. In turn, Al did induce upregulation of non-Tf bound Fe (NTBI) uptake. This modulation was not due to intracellular Fe decrease since NTBI transport proved not to be regulated by Fe depletion. Unlike its behavior in the presence of Tf, Al was unable to compete with NTBI uptake, suggesting that both metals do not share the same alternative transport pathway. We propose that Al interference with TfR-mediated Fe incorporation might trigger the upregulation of NTBI uptake, an adaptation aimed at incorporating the essential metal required for cellular metabolism without allowing the simultaneous access of a potentially toxic metal. © 2004 Elsevier B.V. All rights reserved. Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Risio, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garbossa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674889_v1745_n1_p124_Perez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Aluminum Iron metabolism K562 cell line Non-transferrin bound iron transport Transferrin receptor Transferrin-mediated iron uptake aluminum antigen iron messenger RNA receptor subtype transferrin transferrin receptor article chronic myeloid leukemia controlled study flow cytometry human human cell iron depletion iron transport leukemia cell priority journal protein expression receptor binding regulatory mechanism reverse transcription polymerase chain reaction upregulation Aluminum Biological Transport Butyrates Cell Differentiation Gene Expression Regulation, Neoplastic Humans Iron K562 Cells Receptors, Transferrin RNA, Messenger Transferrin
spellingShingle	Aluminum Iron metabolism K562 cell line Non-transferrin bound iron transport Transferrin receptor Transferrin-mediated iron uptake aluminum antigen iron messenger RNA receptor subtype transferrin transferrin receptor article chronic myeloid leukemia controlled study flow cytometry human human cell iron depletion iron transport leukemia cell priority journal protein expression receptor binding regulatory mechanism reverse transcription polymerase chain reaction upregulation Aluminum Biological Transport Butyrates Cell Differentiation Gene Expression Regulation, Neoplastic Humans Iron K562 Cells Receptors, Transferrin RNA, Messenger Transferrin Pérez, G. Pregi, N. Vittori, D. Di Risio, C. Garbossa, G. Nesse, A. Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
topic_facet	Aluminum Iron metabolism K562 cell line Non-transferrin bound iron transport Transferrin receptor Transferrin-mediated iron uptake aluminum antigen iron messenger RNA receptor subtype transferrin transferrin receptor article chronic myeloid leukemia controlled study flow cytometry human human cell iron depletion iron transport leukemia cell priority journal protein expression receptor binding regulatory mechanism reverse transcription polymerase chain reaction upregulation Aluminum Biological Transport Butyrates Cell Differentiation Gene Expression Regulation, Neoplastic Humans Iron K562 Cells Receptors, Transferrin RNA, Messenger Transferrin
description	Aluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction between both metals prevented normal Fe incorporation into K562 cells and triggered the upregulation of Fe transport. In the present work we demonstrated that Al modified Fe uptake without affecting the expression of Tf receptors. Both TfR and TfR2 mRNA levels, evaluated by RT-PCR, and TfR antigenic sites, analyzed by flow cytometry, were found unchanged after Al exposure. In turn, Al did induce upregulation of non-Tf bound Fe (NTBI) uptake. This modulation was not due to intracellular Fe decrease since NTBI transport proved not to be regulated by Fe depletion. Unlike its behavior in the presence of Tf, Al was unable to compete with NTBI uptake, suggesting that both metals do not share the same alternative transport pathway. We propose that Al interference with TfR-mediated Fe incorporation might trigger the upregulation of NTBI uptake, an adaptation aimed at incorporating the essential metal required for cellular metabolism without allowing the simultaneous access of a potentially toxic metal. © 2004 Elsevier B.V. All rights reserved.
format	Artículo Artículo publishedVersion
author	Pérez, G. Pregi, N. Vittori, D. Di Risio, C. Garbossa, G. Nesse, A.
author_facet	Pérez, G. Pregi, N. Vittori, D. Di Risio, C. Garbossa, G. Nesse, A.
author_sort	Pérez, G.
title	Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_short	Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_full	Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_fullStr	Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_full_unstemmed	Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_sort	aluminum exposure affects transferrin-dependent and -independent iron uptake by k562 cells
publishDate	2005
url	http://hdl.handle.net/20.500.12110/paper_01674889_v1745_n1_p124_Perez
work_keys_str_mv	AT perezg aluminumexposureaffectstransferrindependentandindependentironuptakebyk562cells AT pregin aluminumexposureaffectstransferrindependentandindependentironuptakebyk562cells AT vittorid aluminumexposureaffectstransferrindependentandindependentironuptakebyk562cells AT dirisioc aluminumexposureaffectstransferrindependentandindependentironuptakebyk562cells AT garbossag aluminumexposureaffectstransferrindependentandindependentironuptakebyk562cells AT nessea aluminumexposureaffectstransferrindependentandindependentironuptakebyk562cells
_version_	1769810333403185152

Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells

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