Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8

Mostrar todas las versiones(3)

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pettinari, M.J., Vázquez, G.J., Silberschmidt, D., Rehm, B., Steibüchel, A., Méndez, B.S.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2001
Materias:
acetoacetyl coenzyme a reductase
acetoacetyl-CoA reductase
acetyl coenzyme A acyltransferase
acyltransferase
alcohol dehydrogenase
hydroxybutyric acid
poly beta hydroxybutyrate polymerase
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
poly-beta-hydroxybutyrate polymerase
polyester
article
Azotobacter
bacterial gene
DNA sequence
gene deletion
genetics
growth, development and aging
metabolism
molecular cloning
molecular genetics
Acetyl-CoA C-Acyltransferase
Acyltransferases
Alcohol Oxidoreductases
Cloning, Molecular
Gene Deletion
Genes, Bacterial
Hydroxybutyrates
Molecular Sequence Data
Polyesters
Sequence Analysis, DNA
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00992240_v67_n3-12_p5331_Pettinari
record_format dspace
spelling paperaa:paper_00992240_v67_n3-12_p5331_Pettinari2023-06-12T16:46:20Z Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 Appl. Environ. Microbiol. 2001;67(3-12):5331-5334 Pettinari, M.J. Vázquez, G.J. Silberschmidt, D. Rehm, B. Steibüchel, A. Méndez, B.S. acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vázquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silberschmidt, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic acetoacetyl coenzyme a reductase
acetoacetyl-CoA reductase
acetyl coenzyme A acyltransferase
acyltransferase
alcohol dehydrogenase
hydroxybutyric acid
poly beta hydroxybutyrate polymerase
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
poly-beta-hydroxybutyrate polymerase
polyester
article
Azotobacter
bacterial gene
DNA sequence
gene deletion
genetics
growth, development and aging
metabolism
molecular cloning
molecular genetics
Acetyl-CoA C-Acyltransferase
Acyltransferases
Alcohol Oxidoreductases
Azotobacter
Cloning, Molecular
Gene Deletion
Genes, Bacterial
Hydroxybutyrates
Molecular Sequence Data
Polyesters
Sequence Analysis, DNA
spellingShingle acetoacetyl coenzyme a reductase
acetoacetyl-CoA reductase
acetyl coenzyme A acyltransferase
acyltransferase
alcohol dehydrogenase
hydroxybutyric acid
poly beta hydroxybutyrate polymerase
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
poly-beta-hydroxybutyrate polymerase
polyester
article
Azotobacter
bacterial gene
DNA sequence
gene deletion
genetics
growth, development and aging
metabolism
molecular cloning
molecular genetics
Acetyl-CoA C-Acyltransferase
Acyltransferases
Alcohol Oxidoreductases
Azotobacter
Cloning, Molecular
Gene Deletion
Genes, Bacterial
Hydroxybutyrates
Molecular Sequence Data
Polyesters
Sequence Analysis, DNA
Pettinari, M.J.
Vázquez, G.J.
Silberschmidt, D.
Rehm, B.
Steibüchel, A.
Méndez, B.S.
Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
topic_facet acetoacetyl coenzyme a reductase
acetoacetyl-CoA reductase
acetyl coenzyme A acyltransferase
acyltransferase
alcohol dehydrogenase
hydroxybutyric acid
poly beta hydroxybutyrate polymerase
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
poly-beta-hydroxybutyrate polymerase
polyester
article
Azotobacter
bacterial gene
DNA sequence
gene deletion
genetics
growth, development and aging
metabolism
molecular cloning
molecular genetics
Acetyl-CoA C-Acyltransferase
Acyltransferases
Alcohol Oxidoreductases
Azotobacter
Cloning, Molecular
Gene Deletion
Genes, Bacterial
Hydroxybutyrates
Molecular Sequence Data
Polyesters
Sequence Analysis, DNA
description Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB.
format Artículo
Artículo
publishedVersion
author Pettinari, M.J.
Vázquez, G.J.
Silberschmidt, D.
Rehm, B.
Steibüchel, A.
Méndez, B.S.
author_facet Pettinari, M.J.
Vázquez, G.J.
Silberschmidt, D.
Rehm, B.
Steibüchel, A.
Méndez, B.S.
author_sort Pettinari, M.J.
title Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_short Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_full Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_fullStr Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_full_unstemmed Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_sort poly(3-hydroxybutyrate) synthesis genes in azotobacter sp. strain fa8
publishDate 2001
url http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari
work_keys_str_mv AT pettinarimj poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8
AT vazquezgj poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8
AT silberschmidtd poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8
AT rehmb poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8
AT steibuchela poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8
AT mendezbs poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8
_version_ 1769810226670731264

Ejemplares similares