New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon

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We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors...

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Detalles Bibliográficos
Autores principales: Katzen, F., Becker, A., Ielmini, M.V., Oddo, C.G., Ielpi, L.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 1999
Materias:
xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris pv. campestris
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00992240_v65_n1_p278_Katzen
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spelling paperaa:paper_00992240_v65_n1_p278_Katzen2023-06-12T16:46:20Z New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon Appl. Environ. Microbiol. 1999;65(1):278-282 Katzen, F. Becker, A. Ielmini, M.V. Oddo, C.G. Ielpi, L. xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan. Fil:Katzen, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielmini, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris
Xanthomonas campestris pv. campestris
spellingShingle xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris
Xanthomonas campestris pv. campestris
Katzen, F.
Becker, A.
Ielmini, M.V.
Oddo, C.G.
Ielpi, L.
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
topic_facet xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris
Xanthomonas campestris pv. campestris
description We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.
format Artículo
Artículo
publishedVersion
author Katzen, F.
Becker, A.
Ielmini, M.V.
Oddo, C.G.
Ielpi, L.
author_facet Katzen, F.
Becker, A.
Ielmini, M.V.
Oddo, C.G.
Ielpi, L.
author_sort Katzen, F.
title New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_short New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_full New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_fullStr New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_full_unstemmed New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_sort new mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the xanthomonas campestris pv. campestris gum operon
publishDate 1999
url http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
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