Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The m...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
1986
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo |
| Aporte de: |
| id |
paperaa:paper_00992240_v51_n4_p844_Briozzo |
|---|---|
| record_format |
dspace |
| spelling |
paperaa:paper_00992240_v51_n4_p844_Briozzo2023-06-12T16:46:19Z Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G APPL. ENVIRON. MICROBIOL. 1986;51(4):844-848 Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes. Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water |
| spellingShingle |
botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
| topic_facet |
botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water |
| description |
The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes. |
| format |
Artículo Artículo publishedVersion |
| author |
Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. |
| author_facet |
Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. |
| author_sort |
Briozzo, J. |
| title |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
| title_short |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
| title_full |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
| title_fullStr |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
| title_full_unstemmed |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
| title_sort |
effect of water activity and ph on growth and toxin production by clostridium botulinum type g |
| publishDate |
1986 |
| url |
http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo |
| work_keys_str_mv |
AT briozzoj effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg AT amatodelagardee effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg AT chirifej effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg AT paradajl effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg |
| _version_ |
1769810387142705152 |