α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells

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We report the cloning and characterization of rat α10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the α10 nAChR subunit gene is most similar to the rat α9 nAChR, and both α9 and α10 subunit genes are transcribed in adu...

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Autores principales:	Elgoyhen, A.B., Vetter, D.E., Katz, E., Rothlin, C.V., Heinemann, S.F., Boulter, J.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2001
Materias:	1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cytology female gene expression genetics metabolism molecular genetics mouse physiology Animalia Mammalia Strychnos toxifera Vertebrata Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00278424_v98_n6_p3501_Elgoyhen
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00278424_v98_n6_p3501_Elgoyhen
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spelling	paperaa:paper_00278424_v98_n6_p3501_Elgoyhen2023-06-12T16:45:22Z α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells Proc. Natl. Acad. Sci. U. S. A. 2001;98(6):3501-3506 Elgoyhen, A.B. Vetter, D.E. Katz, E. Rothlin, C.V. Heinemann, S.F. Boulter, J. 1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis We report the cloning and characterization of rat α10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the α10 nAChR subunit gene is most similar to the rat α9 nAChR, and both α9 and α10 subunit genes are transcribed in adult rat mechanosensory hair cells. Injection of Xenopus laevis oocytes with α10 cRNA alone or in pairwise combinations with either α2-α6 or β2-β4 subunit cRNAs yielded no detectable ACh-gated currents. However, coinjection of α9 and α10 cRNAs resulted in the appearance of an unusual nAChR subtype. Compared with homomeric α9 channels, the α9α10 nAChR subtype displays faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions. The pharmacological profiles of homomeric α9 and heteromeric α9α10 nAChRs are essentially indistinguishable and closely resemble those reported for endogenous cholinergic eceptors found in vertebrate hair cells. Our data suggest that efferent modulation of hair cell function occurs, at least in part, through heteromeric nAChRs assembled from both α9 and α10 subunits. Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00278424_v98_n6_p3501_Elgoyhen
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis
spellingShingle	1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis Elgoyhen, A.B. Vetter, D.E. Katz, E. Rothlin, C.V. Heinemann, S.F. Boulter, J. α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
topic_facet	1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis
description	We report the cloning and characterization of rat α10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the α10 nAChR subunit gene is most similar to the rat α9 nAChR, and both α9 and α10 subunit genes are transcribed in adult rat mechanosensory hair cells. Injection of Xenopus laevis oocytes with α10 cRNA alone or in pairwise combinations with either α2-α6 or β2-β4 subunit cRNAs yielded no detectable ACh-gated currents. However, coinjection of α9 and α10 cRNAs resulted in the appearance of an unusual nAChR subtype. Compared with homomeric α9 channels, the α9α10 nAChR subtype displays faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions. The pharmacological profiles of homomeric α9 and heteromeric α9α10 nAChRs are essentially indistinguishable and closely resemble those reported for endogenous cholinergic eceptors found in vertebrate hair cells. Our data suggest that efferent modulation of hair cell function occurs, at least in part, through heteromeric nAChRs assembled from both α9 and α10 subunits.
format	Artículo Artículo publishedVersion
author	Elgoyhen, A.B. Vetter, D.E. Katz, E. Rothlin, C.V. Heinemann, S.F. Boulter, J.
author_facet	Elgoyhen, A.B. Vetter, D.E. Katz, E. Rothlin, C.V. Heinemann, S.F. Boulter, J.
author_sort	Elgoyhen, A.B.
title	α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
title_short	α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
title_full	α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
title_fullStr	α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
title_full_unstemmed	α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
title_sort	α10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
publishDate	2001
url	http://hdl.handle.net/20.500.12110/paper_00278424_v98_n6_p3501_Elgoyhen
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α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells

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